CN-121987518-A - L-carnosine-sophorolipid micelle and preparation method and application thereof

Abstract

The invention belongs to the technical field of skin care products, and relates to an L-carnosine-sophorolipid micelle, a preparation method and application thereof, wherein the method comprises the steps of dissolving sophorolipid in methanol, ethanol or acetone, dissolving L-carnosine in aqueous solution with pH of 2-7, mixing and stirring according to the mass ratio of 3:1-8:1, removing the organic solvent by vacuum rotary evaporation at the temperature of 30-70 ℃ under the pressure of minus 0.06-0.08 MPa to form a film, hydrating by normal saline, purifying by a 1000Da ultrafiltration membrane, and freeze-drying to obtain the product. The obtained micelle has uniform particle size, and the encapsulation rate is up to 99.34%, so that the carnosine enzyme resistance and the transdermal absorption efficiency of the L-carnosine can be improved, and meanwhile, the effects of scavenging free radicals, resisting inflammation, inhibiting bacteria and keeping moisture for a long time are synergistically reserved. The invention has simple and controllable process, solves the problems of poor stability and low bioavailability of the L-carnosine, is suitable for smearing type skin care products, and has important application value.

Inventors

• WANG WANQI
• LUO MAOXING
• CAI JIANFENG

Assignees

• 浙江熙正霖生物科技有限公司

Dates

Publication Date

20260508

Application Date

20260409

Claims (9)

1. 1. The preparation method of the L-carnosine-sophorolipid micelle is characterized by comprising the following steps of: Dissolving sophorolipid in an organic solvent to obtain a sophorolipid solution; dissolving L-carnosine in water to obtain an L-carnosine aqueous solution; mixing sophorolipid solution with L-carnosine aqueous solution; removing the organic solvent to form a film; Hydrating the film with physiological saline to form a micelle solution; and purifying the micelle solution, removing unreacted L-carnosine, and freeze-drying the trapped liquid to obtain the L-carnosine-sophorolipid micelle.
2. 2. The method for preparing L-carnosine-sophorolipid micelles of claim 1, wherein the organic solvent comprises methanol, ethanol and/or acetone.
3. 3. The method for producing an L-carnosine-sophorolipid micelle according to claim 1, wherein the pH of the L-carnosine aqueous solution is 2 to 7.
4. 4. The method for producing an L-carnosine-sophorolipid micelle according to claim 1, wherein the mass ratio of sophorolipid to L-carnosine is 3:1 to 8:1.
5. 5. The method for preparing L-carnosine-sophorolipid micelles of claim 1, wherein the step of removing the organic solvent is vacuum rotary evaporation, the vacuum degree is-0.06 MPa to-0.08 MPa, and the temperature of the vacuum rotary evaporation is 30 ℃ to 70 ℃.
6. 6. The method for preparing L-carnosine-sophorolipid micelles of claim 1, wherein the physiological saline is added in an amount of 1-1.5 times by volume, and the hydration time is 8-12min.
7. 7. The method for preparing L-carnosine-sophorolipid micelles of claim 1, wherein the purifying step comprises ultrafiltration with an ultrafiltration membrane of 1000-1500 Da.
8. 8. An L-carnosine-sophorolipid micelle, characterized by being obtained by the preparation method according to any one of claims 1 to 7.
9. 9. Use of the L-carnosine-sophorolipid micelle of claim 8 in a skin care product.

Description

L-carnosine-sophorolipid micelle and preparation method and application thereof Technical Field The invention belongs to the technical field of skin care products, and particularly relates to an L-carnosine-sophorolipid micelle and a preparation method and application thereof. Background In the field of skin care products, as the demands of consumers for efficient and safe skin care ingredients are increasingly raised, biological active ingredients are receiving attention because of the mildness and functionality. The stability, transdermal absorption efficiency and bioavailability of the active ingredients are core factors for determining the efficacy of skin care products, and most single active ingredients or simple compound systems in the prior art are difficult to meet the requirements at the same time, so that the application effect of the active ingredients in the smearing type skin care products is limited. The L-carnosine is a natural dipeptide composed of beta-alanine and histidine, is used as a novel high-efficiency low-toxicity biological antioxidant, has remarkable skin care value, can effectively remove active oxygen molecules such as superoxide anions and hydroxyl radicals and peroxy radicals, has multiple effects of resisting saccharification, intracellular buffering, tissue repair, improving immune response, resisting apoptosis and the like, and is remarkable in the aspects of preventing skin aging, brightening skin color and the like. However, L-carnosine has inherent technical defects that firstly, as small molecular dipeptide, the L-carnosine is easy to be degraded by specific carnosine enzyme in vivo, so that the stability is insufficient in vitro application, and secondly, the L-carnosine has strong water solubility and weak capability of penetrating through natural biological membrane barriers of skin, so that the bioavailability of the L-carnosine in a smearing type skin care product is low, the full play of the skin care effect of the L-carnosine is seriously influenced, and therefore, the stability and the transdermal performance of the L-carnosine are required to be improved through reasonable structural modification or carrier wrapping technology. Sophorolipids are glycolipid biosurfactants produced by metabolism of yeast, and by virtue of their excellent surface activity, mildness, nonirritating property, anti-inflammatory, antibacterial, moisturizing and other characteristics, the sophorolipids have been widely used as foaming agents, solubilizers, dispersants, detergents and emulsion stabilizers in cosmetics. More importantly, the sophorolipid has a unique amphiphilic structure, can self-assemble to form micelle when the concentration reaches the critical micelle concentration, and the inner cavity structure of the sophorolipid can be used as a carrier of an active ingredient, so that the stability and bioavailability of an active substance are effectively improved, and the sophorolipid is particularly suitable for developing new dosage forms of skin care products such as microcapsule gel, gel beads and micelle, and has obvious advantages in the field of personal care. From the aspect of efficacy cooperativity, the antioxidant and anti-saccharification effects of the L-carnosine have complementarity with the moisturizing, anti-inflammatory and antibacterial effects of sophorolipid and carrier characteristics in theory, and if the L-carnosine can be reasonably combined, the L-carnosine is expected to be protected from degradation, the transdermal absorption capacity of the L-carnosine is enhanced, and the synergic skin care effect of the two components is realized. However, in the prior art, a report on the preparation of a functional skin care carrier by combining L-carnosine and sophorolipid does not appear, and a technical scheme capable of simultaneously solving the problems of poor stability, low transdermal absorption efficiency and the like of the L-carnosine is lacking, so that the requirements of the market on efficient synergistic skin care products cannot be met. In summary, the application of L-carnosine in the prior art is limited by the defects of insufficient stability and poor transdermal absorption, and the carrier potential of sophorolipids is not combined with the skin care efficacy of L-carnosine, so that the technical blank of the combination of the two results in the efficacy of related skin care products to be difficult to break through. Therefore, developing a technical scheme capable of effectively improving the stability and transdermal performance of the L-carnosine and simultaneously playing the synergistic skin care effect of the L-carnosine and the transdermal performance becomes a technical problem to be solved in the field. Disclosure of Invention In order to solve the problems in the background art, the invention provides an L-carnosine-sophorolipid micelle, a preparation method and application thereof, wherein based on self-assembly characteris