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CN-121987520-A - Supermolecular enzyme solution and preparation method and application thereof

CN121987520ACN 121987520 ACN121987520 ACN 121987520ACN-121987520-A

Abstract

The invention provides a supermolecular enzyme solution, a preparation method and application thereof, and belongs to the technical field of cosmetics. The technical problem to be solved is that protease, superoxide dismutase and other enzymes have poor stability, so that the protease, superoxide dismutase and other enzymes are easy to be inactivated or denatured under the influence of temperature, pH value change, ionic strength, interfacial tension and preservative in the preparation, storage and use environments of enzyme preparations and other products. The technical scheme is that the supermolecular enzyme solution comprises enzyme and a supermolecular solvent, wherein the supermolecular solvent comprises a hydrogen bond acceptor and a hydrogen bond donor in a molar ratio of 1-4:1-4, the hydrogen bond acceptor is a beet root extract, the hydrogen bond acceptor is an aromatic-free organic acid, the aromatic-free organic acid comprises one or more of citric acid, lactic acid, malic acid, succinic acid and glycolic acid, and when the aromatic-free organic acid comprises citric acid and/or malic acid, the supermolecular enzyme solution further comprises water.

Inventors

  • ZHANG ZIWU
  • HUANG YING
  • XIANG WENHAO
  • ZENG FUQIANG
  • LIN CHUN
  • JI NING

Assignees

  • 广州科盈化妆品有限公司

Dates

Publication Date
20260508
Application Date
20260409

Claims (10)

  1. 1. A supermolecular enzyme solution is characterized by comprising an enzyme and a supermolecular solvent, wherein the supermolecular solvent comprises a hydrogen bond acceptor and a hydrogen bond donor in a molar ratio of 1-4:1-4, the hydrogen bond acceptor is a beet root extract, and the hydrogen bond donor is an aromatic-free organic acid, and the aromatic-free organic acid comprises one or more of citric acid, lactic acid, malic acid, succinic acid and glycolic acid; Wherein when the non-aromatic organic acid comprises citric acid and/or malic acid, the supermolecular enzyme solution further comprises water; the preparation of the supramolecular enzyme solution comprises the following steps: (1) Mixing a hydrogen bond acceptor and a hydrogen bond donor, or mixing the hydrogen bond acceptor and the hydrogen bond donor, then adding the mixture into water, and heating and stirring uniformly under the protection of inert gas to obtain a mixed solution; (2) And (3) cooling the mixed solution, adding enzyme under the protection of inert gas, stirring at a controlled temperature, and performing ultrasonic treatment to obtain the supermolecular enzyme solution.
  2. 2. The supramolecular enzyme solution according to claim 1, wherein the non-aromatic organic acid is citric acid, lactic acid or succinic acid.
  3. 3. The supramolecular enzyme solution according to claim 1, wherein the enzyme is present in an amount of 0.1% (w/v) to 5% (w/v) of the supramolecular solvent.
  4. 4. The supramolecular enzyme solution according to claim 1, wherein when the non-aromatic organic acid comprises citric acid and/or malic acid, the supramolecular enzyme solution further comprises water in an amount of 10% -40% by mass of the supramolecular solvent.
  5. 5. The supramolecular enzyme solution according to claim 1, wherein the enzyme is selected from one or more of papain, bromelain, ficin, subtilase, superoxide dismutase, lysozyme, coenzyme Q10 and DNA repair enzyme.
  6. 6. The method for preparing a supramolecular enzymatic solution according to any one of claims 1-5, comprising the steps of: (1) Mixing a hydrogen bond acceptor and a hydrogen bond donor, or mixing the hydrogen bond acceptor and the hydrogen bond donor, then adding the mixture into water, and heating and stirring uniformly under the protection of inert gas to obtain a mixed solution; (2) And (3) cooling the mixed solution, adding enzyme under the protection of inert gas, stirring at a controlled temperature, and performing ultrasonic treatment to obtain the supermolecular enzyme solution.
  7. 7. The method according to claim 6, wherein the inert gas is selected from helium, argon or nitrogen, and the inert gas has a pressure of 5 to 20MPa.
  8. 8. The method according to claim 6, wherein in the step (1), the temperature of the heating and stirring is 60-100 ℃, the rotation speed is 50-200rpm, and the time is 6-18 hours; And/or in the step (2), the temperature of the cooling and temperature control is 25-45 ℃; and/or in the step (2), the power of the ultrasonic treatment is 200-600W, the frequency is 10-30kHz, and the time is 1-3h.
  9. 9. Use of the supramolecular enzyme solution according to any one of claims 1-5 or the supramolecular enzyme solution prepared by the preparation method according to any one of claims 6-8 in cosmetics.
  10. 10. The use according to claim 9, wherein the cosmetic formulation comprises an aqueous, an essence or an emulsion.

Description

Supermolecular enzyme solution and preparation method and application thereof Technical Field The invention belongs to the technical field of cosmetics, and provides a supermolecular enzyme solution, a preparation method and application thereof. Background The problem of pore blockage and acne with closed mouth caused by vigorous sebum secretion and abnormal cutin metabolism is generally faced by oily skin people, and currently, the main stream adopts a chemical method of brushing acid such as fruit acid (AHA), salicylic acid (BHA) and the like, and the action mechanism of the acid is non-selective to dissolve cutin, so that skin is easy to dry, reddish and glory, even the skin barrier function is damaged, and sensitive discomfort is caused. The enzyme as a high-efficiency and specific biocatalyst has great application potential in the field of cosmetics. For example, proteases (such as papain and bromelain) can specifically hydrolyze desmin linked to keratinocytes, thereby achieving mild and precise exfoliating effects, and superoxide dismutase (SOD) can scavenge free radicals and provide excellent antioxidant effects. Compared with chemical antioxidant and exfoliating components (if acids and salicylic acid), the action mechanism of the enzyme is milder, and the excessive damage to skin barriers can be reduced theoretically, so that the irritation risk is reduced. However, their use in cosmetics is still facing serious challenges due to enzyme instability. The activity of enzymes is highly dependent on their specific three-dimensional structure and is susceptible to inactivation or denaturation by temperature, pH changes, ionic strength, interfacial tension, and preservatives in the usual cosmetic preparation, storage, and use environments. This results in a product containing enzyme activity that decays rapidly during shelf life, severely limiting its commercial application and market acceptance. The Chinese patent application CN116376882A (publication day: 2023.7.4) discloses a method for extracting papain by using a low-temperature plasma assisted eutectic solvent, which comprises the steps of mixing papain peel with an aqueous solution of the eutectic solvent, performing low-temperature plasma extraction to obtain an extracting solution, adding a cysteine salt protective agent into the extracting solution, and performing multistage membrane separation and freeze drying to obtain papain, wherein the eutectic solvent comprises a hydrogen bond donor and a hydrogen bond acceptor, the hydrogen bond acceptor is choline chloride, the hydrogen bond donor is glycerol, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor of the eutectic solvent is 1:3, and the preparation method of the eutectic solvent comprises the steps of mixing the hydrogen bond acceptor and the hydrogen bond donor according to the molar ratio of 1:3, and stirring for 1.5h at 60-80 ℃. The eutectic solvent adopted by the method has good solubility for protein, but the stability of the protein in the eutectic solvent still needs to be further improved. The Chinese patent application CN120858973A (publication day: 2025.10.31) discloses a passivation enzyme soluble agent and a preparation process thereof, wherein the passivation enzyme soluble agent comprises the following components of composite passivation enzyme freeze-dried powder, plant phloem conduction enhancer, biological source permeation enhancer and multifunctional deep eutectic solvent, and concretely discloses the multifunctional deep eutectic solvent is a nonaqueous solvent prepared by mixing a hydrogen bond acceptor, a first hydrogen bond donor, a second hydrogen bond donor and a redox buffer pair according to a preset molar ratio, wherein the hydrogen bond acceptor is choline chloride, the first hydrogen bond donor is L-lactic acid, and the second hydrogen bond donor is L-malic acid. The problems of unstable storage and easy loss of activity of the traditional water-based enzyme preparation are fundamentally solved by constructing an anhydrous environment and adopting an activity protection process. However, when the passivated enzyme soluble solution is applied to products such as cosmetics, the pH balance of the passivated enzyme soluble solution system is easily destroyed due to the existence of redox substances in the product formulation, thereby affecting the stability of the enzyme. Therefore, further researches on solvent systems of proteases are necessary to achieve that proteases can maintain higher stability under different temperature and pH conditions or in the absence of preservative, redox agent and other substances, and further improve shelf life and efficacy of protease-related products. Disclosure of Invention The invention aims to provide that: A supermolecular enzyme solution and its relative technology are disclosed, which can solve the technical problems that the stability of protease, superoxide dismutase and other enzymes is poor, so th