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CN-121987521-A - Coated liposome and preparation method and application thereof

CN121987521ACN 121987521 ACN121987521 ACN 121987521ACN-121987521-A

Abstract

The invention provides a coated liposome and a preparation method and application thereof, and belongs to the technical field of cosmetics. The preparation method comprises the steps of (1) mixing ceramide 3, dimer linolic acid ester and cholesterol, heating and melting, cooling, adding ergothioneine, stirring and mixing to obtain an oil phase, (2) heating and mixing polyquaternium-51, sodium bis (lauramide glutamine) lysine, sodium hyaluronate, glycerol and water to obtain a water phase, (3) adding the oil phase into the water phase in a fine flow mode under heating conditions, homogenizing by ultra-high pressure microjet, and (4) adding bis-PEG-18 methyl ether dimethyl silane and pre-dispersed poly (acryl dimethyl sodium taurate) in a temperature-controlled and temperature-reduced mode, stirring and mixing to obtain a coated liposome, wherein the water-adding agent is not turbid, has good high and low temperature stability, and has synergistic skin targeted delivery and skin barrier repairing effects.

Inventors

  • Sang Yongshuai
  • LI XIAOHU
  • ZHANG JING
  • ZHANG GUANGHONG
  • CHENG WEIFENG
  • YANG JU

Assignees

  • 上海美兮美学生物医药科技有限公司
  • 上海奥利实业有限公司
  • 杭州美兮美学生物科技有限公司

Dates

Publication Date
20260508
Application Date
20260317

Claims (9)

  1. 1. A method for preparing a entrapped liposome, comprising the steps of: (1) Mixing ceramide 3, dimer linoleate and cholesterol, heating for melting, cooling, adding ergothioneine, stirring and mixing to obtain supermolecule pre-composite oil phase; (2) Heating and stirring polyquaternium-51, sodium bis (lauramide glutamine) lysine, sodium hyaluronate, glycerol and water uniformly to obtain a lipid matrix water phase between the cells; (3) Under the heating condition, adding the supermolecule pre-composite oil phase into the intercellular lipid matrix water phase in a trickle form, and homogenizing by ultra-high pressure micro-jet; (4) And (3) temperature control, speed reduction and cooling, adding bis-PEG-18 methyl ether dimethyl silane and pre-dispersed poly (acryl dimethyl taurine) sodium, stirring and mixing to obtain the encapsulated liposome, wherein the speed of temperature control, speed reduction and cooling is 0.3-0.5 ℃ per minute, and the temperature is reduced to 40-45 ℃.
  2. 2. The method according to claim 1, wherein the heating temperature in step (1) is 120 to 130 ℃, and the cooling temperature is 90 to 100 ℃.
  3. 3. The preparation method according to claim 1, wherein the mass ratio of ceramide 3, dimer linoleate, cholesterol and ergothioneine is 2-5:0.5-2:0.5-1.5:0.1-0.15.
  4. 4. The method according to claim 1, wherein the heating in step (2) is at a temperature of 80 to 85 ℃.
  5. 5. The preparation method according to claim 1, wherein the mass ratio of the polyquaternium-51, the sodium bis (lauramide glutamine) lysine, the sodium hyaluronate, the glycerol and the water is 5-10:0.1-2:1-2:5-10:30-60.
  6. 6. The method according to claim 1, wherein the heating temperature in step (3) is 85-95 ℃, the pressure of the ultra-high pressure micro-jet homogenization is 1200-1500bar, and the cycle is 2-4 times.
  7. 7. The preparation method according to claim 1, wherein the mass ratio of the bis-PEG-18 methyl ether dimethylsilane to the pre-dispersed sodium polyacryl dimethyltaurate is 0.1-0.3:0.2-1.
  8. 8. A entrapped liposome prepared by the method of any one of claims 1-7.
  9. 9. Use of the encapsulated liposome according to claim 8 for the preparation of skin care products, make-up bases, make-up products, medical repair products.

Description

Coated liposome and preparation method and application thereof Technical Field The invention relates to the technical field of cosmetics, in particular to a coated liposome and a preparation method and application thereof. Background The impaired skin barrier function is closely related to the occurrence and development of many skin diseases, particularly facial skin diseases represented by rosacea, sensitive skin, seborrheic dermatitis, and the like. The skin barrier function is impaired, and the facial skin is susceptible to symptoms, which are subjective symptoms such as dryness, burning, itching, stinging and the like, or objective symptoms such as desquamation, erythema, telangiectasia and the like. Repairing the skin barrier function is of great importance for the relief of the above-mentioned symptoms of facial skin sensitivity and for the treatment of these diseases. Ceramide, an important constituent of intercellular lipids of the stratum corneum, plays a non-negligible role in maintaining the normal physiological functions of the skin. Ceramides are a class of polar lipid molecules consisting of a long chain base of sphingosine and a fatty acid, which form ceramides of varying properties depending on chain length, type and degree of hydroxylation, saturation, etc. Ceramide is a compound which is easy to self-assemble to form crystals, is relatively inconvenient to directly apply to cosmetics, and is currently pretreated by various technologies, such as liposome encapsulation, submicron liposome encapsulation, polymer microcapsule encapsulation and the like, so as to improve the stability and bioavailability of the ceramide in the products. Liposomes are now widely accepted carrier pharmaceutical formulations, and after application to the skin using liposomes as a carrier, the drug penetrates the stratum corneum more readily into the skin. The prior commercially available ceramide products subjected to the encapsulation treatment mainly have the following two problems of 1) the turbidity phenomenon when part of the commercially available encapsulated ceramide is added into the water aqua, and 2) the abnormality easily occurs when part of the commercially available encapsulated ceramide is added into the water aqua, such as floccules and white fine particles under the low-temperature condition and the turbidity phenomenon under the high-temperature condition, when the stability is inspected. The reason for this is that ceramide has an extremely high melting point and a strong intermolecular hydrogen bond network, and self-assembled crystallization is easily induced. Disclosure of Invention The invention aims to provide a coated liposome and a preparation method and application thereof, solves the problem that the traditional ceramide liposome is easy to generate aqua turbidity, has better high-low temperature stability, and has the synergistic skin targeting delivery effect, the skin barrier repairing effect is obviously improved, and the application prospect is wide. The technical scheme of the invention is realized as follows: The invention provides a preparation method of a coated liposome, which comprises the following steps: (1) Mixing ceramide 3, dimer linoleate and cholesterol, heating for melting, cooling, adding ergothioneine, stirring and mixing to obtain supermolecule pre-composite oil phase; The invention utilizes the characteristic that ergothioneine and ceramide can form a thioketone-amide hydrogen bond supermolecule to destroy the regular crystallization tendency of ceramide 3, in addition, dimer linolic acid ester has irregular cyclic/branched side chains, is inserted among ceramide molecules to destroy the regular lamellar accumulation, prevents the ceramide from being recrystallized due to surface tension aggregation at high temperature, and ensures that the uniform liquid phase environment is used for molecular diffusion when the ergothioneine is added. The hydroxyl of cholesterol can form hydrogen bond with amide of ceramide 3 and thioketone of ergothioneine, so as to construct supermolecule pre-complex. (2) Heating and stirring polyquaternium-51, sodium bis (lauramide glutamine) lysine, sodium hyaluronate, glycerol and water uniformly to obtain a lipid matrix water phase between the cells; The inventor of the invention researches and discovers that the ergothioneine is easy to oxidize and unstable, so that the ergothioneine is stabilized by adding sodium hyaluronate, and meanwhile, the sodium hyaluronate has better moisturizing, antioxidant, anti-saccharification and repairing effects and slowly releases the sodium hyaluronate, thereby realizing the effect of improving the skin for a long time. Sodium bis (lauramide glutamine) lysine is a peptide-based gemini surfactant, has good skin penetration promoting performance, can interact with ceramide molecules to form a nested structure, and damages the ordered arrangement of the ceramide molecules, thereby inhibiting self-assembled