CN-121987522-A - Composition containing DNA fragment and application of lidocaine hydrochloride

Abstract

The application provides a composition containing a DNA fragment and application of lidocaine hydrochloride in improving the stability of the composition containing the DNA fragment and crosslinked hyaluronic acid. Wherein the composition comprises cross-linked hyaluronic acid, a DNA fragment and lidocaine hydrochloride. The application discovers that lidocaine hydrochloride can improve the stability of PDRN or PN in the storage process of a crosslinked hyaluronic acid system. In addition, the viscoelasticity of the crosslinked hyaluronic acid has an important influence on the stability of the PDRN or PN in a crosslinked hyaluronic acid system, and when the crosslinked hyaluronic acid with specific elastic modulus and viscous modulus is combined with lidocaine hydrochloride and the PDRN or PN, the storage stability of the PDRN or PN in the crosslinked hyaluronic acid system can be remarkably improved, so that the combined product can stably maintain a uniform and transparent state for a long time, and the effects of resisting inflammation, repairing and promoting collagen synthesis are improved.

Inventors

• YANG YINGYING
• ZHANG XIANGLONG
• ZHANG YAN
• LU LILI
• CHEN WENWEN
• LI MAN
• SU YANG
• LIU JIANJIAN
• YANG SHENGLIN
• LIU XU

Assignees

• 华熙生物科技(海南)有限公司
• 华熙生物科技股份有限公司

Dates

Publication Date

20260508

Application Date

20241108

Claims (9)

1. 1. A composition comprising DNA fragments comprising cross-linked hyaluronic acid, DNA fragments and lidocaine hydrochloride.
2. 2. The composition according to claim 1, wherein the crosslinked hyaluronic acid is a gel obtained by modifying hyaluronic acid or a salt thereof with a crosslinking agent under alkaline conditions, preferably the crosslinked hyaluronic acid has a modification degree of 0.2% to 2.2%.
3. 3. The composition of claim 1 or 2, wherein the crosslinked hyaluronic acid has an elastic modulus of 50-400 Pa-S and a viscous modulus of 20-120 Pa-S at a test frequency of 0.1 Hz.
4. 4. A composition according to claim 2 or 3, wherein the mass ratio of hyaluronic acid or a salt thereof, DNA fragment and lidocaine hydrochloride in the composition is (5-30): 2-10): 2-4.
5. 5. The composition of any one of claims 1-4, wherein the DNA fragment comprises one or both of a polydeoxyribonucleotide, a polynucleotide.
6. 6. The composition of claim 5, wherein the polydeoxyribonucleotide has a molecular weight of 5-90 ten thousand and the polynucleotide has a molecular weight of 100-150 ten thousand.
7. 7. The composition according to any one of claims 2 to 6, wherein in the composition, the content of hyaluronic acid or a salt thereof is 4-32mg/ml, the content of DNA fragment is 1-12mg/ml, and the content of lidocaine hydrochloride is 1-5mg/ml.
8. 8. Use of lidocaine hydrochloride for improving the stability of a composition comprising DNA fragments and cross-linked hyaluronic acid.
9. 9. Use of a composition according to any one of claims 1 to 7 for the preparation of a cosmetic medical product for promoting wound healing.

Description

Composition containing DNA fragment and application of lidocaine hydrochloride Technical Field The application belongs to the technical field of medical cosmetology, and particularly relates to a composition containing a DNA fragment and application of lidocaine hydrochloride in improving stability of an injection composition containing the DNA fragment and crosslinked hyaluronic acid. Background Along with economic development and social progress, people attach more importance to the external image. The skin is the largest organ on the outermost surface of the human body, and can be aged gradually like other organs of the human body, so that the functions are weakened or lost, the beauty is affected, and the incidence rate of various skin diseases is increased. In addition, the skin has stronger psychological effect due to the superficial exposure, and the delay of skin aging is one of the hot spots of people. Especially, the beauty and younger of the micro wound surface is sought by a plurality of beauty-seeking persons, and is also one of research hotspots in the domestic and foreign life science fields. Injection beauty is a common minimally invasive beauty means, and can directly inject nutrient substances required by skin into the skin, and after the nutrient substances are directly and rapidly absorbed through subcutaneous tissues, the effects of resisting aging and regenerating the skin are achieved. Crosslinked hyaluronic acid (hyalouronic acid, HA) is a commonly used injectable cosmetic material. Sensitive muscles, anti-inflammatory, repairing, red removing, color spot and acne marks and the like are high-potential medical requirements. DNA fragments, such as Polydeoxyribonucleotides (PDRNs) or Polynucleotides (PN), are components that contribute to skin anti-inflammatory, repair and collagen regeneration. However, the mixed system of the crosslinked sodium hyaluronate gel and the PDRN or PN is a polymer material slightly dissolved in water, so that the mixed system can be separated out from the gel along with the extension of the storage time or the change of the storage condition after being dissolved in an isotonic physiological buffer solution, and the gel becomes turbid, thereby influencing the use effect of the product. Disclosure of Invention The application provides a composition containing DNA fragments and application of lidocaine hydrochloride in improving stability of a composition for injection containing the DNA fragments and crosslinked hyaluronic acid. In particular, the application relates to the following aspects: the application provides a composition containing DNA fragments, comprising cross-linked hyaluronic acid, DNA fragments and lidocaine hydrochloride. The crosslinked hyaluronic acid is gel obtained by modifying hyaluronic acid or salt thereof with a crosslinking agent under alkaline conditions, preferably, the modification degree of the crosslinked hyaluronic acid is 0.2% -2.2%, and most preferably, the modification degree of the crosslinked hyaluronic acid is 0.3% -2%. Further, at a test frequency of 0.1Hz, the crosslinked hyaluronic acid has an elastic modulus of 50-400 Pa.S and a viscous modulus of 20-120 Pa.S. Further, in the composition, the mass ratio of the hyaluronic acid or a salt thereof, the DNA fragment and the lidocaine hydrochloride is (5-30): 2-10): 2-4. Further, the DNA fragment comprises one or two of polydeoxyribonucleotide and polynucleotide. Further, the molecular weight of the polydeoxyribonucleotide is 5 ten thousand to 90 ten thousand, and the molecular weight of the polynucleotide is 100 ten thousand to 150 ten thousand. Further, in the composition, the content of the hyaluronic acid or the salt thereof is 4-32mg/ml, the content of the DNA fragment is 1-12mg/ml, and the content of lidocaine hydrochloride is 1-5mg/ml. The application further provides the use of lidocaine hydrochloride to improve the stability of a composition comprising a DNA fragment and cross-linked hyaluronic acid. The crosslinked hyaluronic acid is gel obtained by modifying hyaluronic acid or salt thereof with a crosslinking agent under alkaline conditions, and preferably, the modification degree of the crosslinked hyaluronic acid is 0.3% -2%. Further, at a test frequency of 0.1Hz, the crosslinked hyaluronic acid has an elastic modulus of 50-400 Pa.S and a viscous modulus of 20-120 Pa.S. Further, in the composition, the mass ratio of the hyaluronic acid or the salt thereof, the DNA fragment and the lidocaine hydrochloride is 5-30:2-10:2-4. Further, the DNA fragment comprises one or two of polydeoxyribonucleotide and polynucleotide. Further, the molecular weight of the polydeoxyribonucleotide is 5 ten thousand to 90 ten thousand, and the molecular weight of the polynucleotide is 100 ten thousand to 150 ten thousand. Further, in the composition, the content of the hyaluronic acid or the salt thereof is 4-32mg/ml, the content of the DNA fragment is 1-12mg/ml, and the content of li