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CN-121987544-A - Tea polyphenol blend and preparation method and application thereof

CN121987544ACN 121987544 ACN121987544 ACN 121987544ACN-121987544-A

Abstract

The invention discloses a tea polyphenol blend, a preparation method and application thereof, and the tea polyphenol blend comprises, by weight, 0.2-3 parts of tea polyphenol, 0.5-5 parts of protein-silk fibroin peptide powder and 0.2-5 parts of pullulan. The method can solve the problems that the tea polyphenol is easy to generate oxidative polymerization to generate color change and active ingredients are degraded in the prior art, so that the problem that the adding content of the tea polyphenol in cosmetics is very low and is always in a trace adding range and cannot be widely applied to cosmetics is solved, and the application performance of the tea polyphenol is improved.

Inventors

  • TANG LIRONG
  • Gai Peipei
  • ZHENG TING
  • LI XIAOXIA
  • GUO CHUNJING
  • ZHANG SHENGWEI
  • YU YANXIA
  • ZHOU JIADE

Assignees

  • 杭州千岛湖天鑫有限公司

Dates

Publication Date
20260508
Application Date
20260407

Claims (10)

  1. 1. The tea polyphenol blend is characterized by comprising, by weight, 0.2-3 parts of tea polyphenol, 0.5-5 parts of protein-silk fibroin peptide powder and 0.2-5 parts of pullulan.
  2. 2. The tea polyphenol blend according to claim 1, wherein the protein-silk fibroin peptide powder is obtained by uniformly mixing silk fibroin and soluble silk fibroin peptide powder in a weight ratio of 7:3.
  3. 3. A method for preparing a tea polyphenol blend, comprising the steps of: S1, purifying degummed silk to obtain silk fibroin powder, and hydrolyzing silk fibroin to obtain soluble silk fibroin peptide powder with a molecular weight of 450-2000, wherein the sequence is Ala-Ser-Gly-Ser-Ala; s2, uniformly mixing silk fibroin and soluble silk fibroin peptide powder in a weight ratio of 7:3 for standby use to obtain protein-silk fibroin peptide powder; S3, dissolving pullulan into water to prepare a solution with the mass fraction of 20%, and placing the solution at the temperature of 4 ℃ for refrigeration for later use; S4, mixing the protein-silk fibroin peptide powder and the glycerol in a weight ratio of 1:5, and uniformly stirring and mixing at 50-70rpm at 20-30 ℃ to form a product A with uniformity; S5, slowly adding tea polyphenol into the A product, and stirring at 50-70rpm under the condition of 20-30 ℃ until the tea polyphenol and the A product are uniformly mixed to obtain a B product, wherein the weight ratio of the tea polyphenol to the protein-silk peptide powder in the A product is 1:2; S6, regulating the pH of the product B to 3.5-4.0 by using citric acid, slowly dripping the pullulan solution into the product B, and stirring until the pullulan solution and the product B are completely mixed to obtain a product C, wherein the dry weight ratio of the pullulan to the protein-silk peptide powder in the product B is 1:1; S7, placing the obtained C product in a centrifuge tube at a rotating speed of 10000rpm after refrigerating for 24 hours at 4 ℃, centrifuging for 10 minutes, removing the uppermost layer and the lowermost layer of clear liquid, and collecting the residual liquid in the centrifuge tube to obtain a D product; s8, placing the D product in an ultrasonic instrument, adjusting the output power to be 20kHz, and the working time to be 10S, and carrying out ice bath treatment for 10 minutes every 2S to obtain the finished tea polyphenol blend.
  4. 4. The use of a tea polyphenol blend as an active ingredient in the cosmetic field.
  5. 5. The use of a tea polyphenol blend as claimed in claim 4 as an active ingredient in the cosmetic field, wherein the cosmetic type comprises essence, emulsion and face cream.
  6. 6. Use of a tea polyphenol blend according to claim 5 as active ingredient in the cosmetic field, characterized in that: the cosmetic is a cosmetic with multiple emulsions of W/O/W emulsion.
  7. 7. The method of claim 5, wherein the cosmetic comprises a first aqueous phase, a fatty phase and a second aqueous phase.
  8. 8. The method of claim 7, wherein the first aqueous phase comprises 10 parts by weight of the tea polyphenol blend and 10 parts by weight of deionized water.
  9. 9. The method of claim 7, wherein the second aqueous phase comprises 50 parts by weight of water, 1 part by weight of phenoxyethanol, 0.5 part by weight of 1, 2-hexanediol, 2 parts by weight of PEG-100 stearate, 0.1 part by weight of daily essence, 10 parts by weight of glycerol and 0.5 part by weight of carbomer.
  10. 10. The use of a tea polyphenol blend as claimed in claim 7 as an active ingredient in the cosmetic field, wherein the fatty phase comprises, in parts by weight, 10 parts of squalane, 5 parts of cetostearyl alcohol, 5 parts of caprylic/capric triglyceride, 0.5 part of tocopherol and 2 parts of glyceryl stearate.

Description

Tea polyphenol blend and preparation method and application thereof Technical Field The invention relates to a blend and a preparation method and application thereof, in particular to a tea polyphenol blend and a preparation method and application thereof. Background Tea polyphenols, also known as tea tannins, are a generic name of a class of polyhydroxy phenolic compounds contained in tea, and the components comprise catechins (flavanols), flavones, flavonols, anthocyanidins, phenolic acids, condensed phenolic acids, polymerized phenols and the like, wherein the catechins are main components of the tea polyphenols, and comprise catechin (C), epicatechin (EC), epicatechin gallocatechin (ECG) and the like. The oxidative polymerization of tea polyphenol is mainly determined by the influence of both tea polyphenol oxidase and non-oxidase, wherein the tea polyphenol oxidase is a secondary metabolite existing in tea trees, but the tea polyphenol oxidase is always remained in the high-temperature drying powder spraying process in the extraction process of tea polyphenol raw materials, so that the oxidative browning of tea polyphenol in the application process of cosmetics often causes the change of the tea polyphenol to polymer due to the oxidative polymerization reaction of non-enzyme, the condition that the active ingredients of the tea polyphenol are degraded in the polymerization process, the polymerization reaction is irreversible after the occurrence, if a learner researches that the thermal stability of the tea polyphenol is poor, the gradual decline trend of the content of the tea polyphenol is generated along with the rise of the temperature, the problems that the tea polyphenol has strong biological activity but is unstable in structure and is very easy to generate discoloration and active ingredient degradation are solved, the tea polyphenol cannot be widely applied to cosmetics or food industries, the content of the tea polyphenol in the cosmetic industry is extremely low, and the stability of the tea polyphenol is difficult to be ensured in the cosmetic industry when the content of the tea polyphenol is contained in the trace amount. Disclosure of Invention The invention aims to provide a tea polyphenol blend, a preparation method and application thereof. The method can solve the problems that the tea polyphenol is easy to generate oxidative polymerization to generate color change and active ingredients are degraded in the prior art, so that the problem that the adding content of the tea polyphenol in cosmetics is very low and is always in a trace adding range and cannot be widely applied to cosmetics is solved, and the application performance of the tea polyphenol is improved. The technical scheme of the invention is that the tea polyphenol blend comprises, by weight, 0.2-3 parts of tea polyphenol, 0.5-5 parts of protein-silk fibroin peptide powder and 0.2-5 parts of pullulan. In the tea polyphenol blend, the protein-silk fibroin peptide powder is obtained by uniformly mixing silk fibroin and soluble silk fibroin peptide powder in a weight ratio of 7:3. A method for preparing a tea polyphenol blend, comprising the steps of: S1, purifying degummed silk to obtain silk fibroin powder, and hydrolyzing silk fibroin to obtain soluble silk fibroin peptide powder with a molecular weight of 450-2000, wherein the sequence is Ala-Ser-Gly-Ser-Ala; s2, uniformly mixing silk fibroin and soluble silk fibroin peptide powder in a weight ratio of 7:3 for standby use to obtain protein-silk fibroin peptide powder; S3, dissolving pullulan into water to prepare a solution with the mass fraction of 20%, and placing the solution at the temperature of 4 ℃ for refrigeration for later use; S4, mixing the protein-silk fibroin peptide powder and the glycerol in a weight ratio of 1:5, and uniformly stirring and mixing at 50-70rpm at 20-30 ℃ to form a product A with uniformity; S5, slowly adding tea polyphenol into the A product, and stirring at 50-70rpm under the condition of 20-30 ℃ until the tea polyphenol and the A product are uniformly mixed to obtain a B product, wherein the weight ratio of the tea polyphenol to the protein-silk peptide powder in the A product is 1:2; S6, regulating the pH of the product B to 3.5-4.0 by using citric acid, slowly dripping the pullulan solution into the product B, and stirring until the pullulan solution and the product B are completely mixed to obtain a product C, wherein the dry weight ratio of the pullulan to the protein-silk peptide powder in the product B is 1:1; S7, placing the obtained C product in a centrifuge tube at a rotating speed of 10000rpm after refrigerating for 24 hours at 4 ℃, centrifuging for 10 minutes, removing the uppermost layer and the lowermost layer of clear liquid, and collecting the residual liquid in the centrifuge tube to obtain a D product; s8, placing the D product in an ultrasonic instrument, adjusting the output power to be 20kHz, and the working time to be 1