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CN-121987548-A - Biological agent for deodorizing and tooth strengthening of pet oral cavity and preparation method thereof

CN121987548ACN 121987548 ACN121987548 ACN 121987548ACN-121987548-A

Abstract

The application relates to the field of daily necessities and biotechnology of pets, and discloses a biological agent for deodorizing and tooth-fixing of the oral cavity of the pet and a preparation method thereof. The microbial active component is prepared by controlling the coupling symbiotic fermentation process of inoculation time sequence, so that the stress resistance of the strain and the expression quantity of an extracellular enzyme system are improved, and the plant extract is modified by enzymolysis and bioconversion, so that the polymerization degree and the irritation are reduced, and the flocculation antagonism problem of polyphenol substances and living bacteria is solved. The product of the application is divided into two dosage forms of spray and dentifrice, utilizes the synergistic mechanism of biological enzymolysis to destroy dental plaque biomembrane and chemical complexation to remove sulfide, and has the characteristics of high deodorization efficiency, high viable bacteria survival rate and good biocompatibility.

Inventors

  • Long Kuicheng
  • YUE SHOUSONG

Assignees

  • 山东康贝尔生物科技有限公司

Dates

Publication Date
20260508
Application Date
20251216

Claims (10)

  1. 1. The biological agent for deodorizing and strengthening teeth of the pet oral cavity is characterized by being prepared from the following raw materials in percentage by weight: 18.0 to 50.0 percent of microorganism active component; 4.0 to 12.0 percent of modified persimmon tannin extract; 2.0 to 5.0 percent of modified honeysuckle extract; 2.0 to 6.0 percent of isomaltooligosaccharide; The balance being a carrier matrix; The microbial active components are time sequence coupling symbiotic fermentation stock solution and composite probiotic dry powder prepared from the time sequence coupling symbiotic fermentation stock solution; The modified persimmon tannin extract is a modified persimmon tannin extract and dry powder prepared from the modified persimmon tannin extract; the modified honeysuckle extract is modified honeysuckle extract and dry powder prepared from the modified honeysuckle extract.
  2. 2. The biological agent for deodorizing and strengthening teeth in the oral cavity of a pet according to claim 1, wherein the biological agent is a spray, and the raw materials of the biological agent comprise the following components: 45.0 to 50.0 percent of time sequence coupling symbiotic fermentation stock solution; 10.0 to 12.0 percent of modified persimmon tannin extract; 4.0 to 5.0 percent of modified honeysuckle extract; 2.0 to 3.0 percent of isomaltooligosaccharide; 0.1 to 0.2 percent of meat powder hydrolysate; 0.5 to 0.8 percent of buffer solution of citric acid and sodium citrate; the balance being a carrier matrix consisting of food grade glycerol and purified water.
  3. 3. The biological agent for deodorizing and strengthening teeth in the oral cavity of a pet according to claim 1, wherein the biological agent is tooth cleaning powder, and the raw materials comprise the following components: 18.0 to 22.0 percent of composite probiotic dry powder; 4.0 to 5.0 percent of modified persimmon tannin extract dry powder; 2.0 to 2.5 percent of modified honeysuckle extract dry powder; 5.0 to 6.0 percent of isomaltooligosaccharide; 35.0 to 38.0 percent of food-grade calcium silicate; 29.0 to 33.5 percent of freeze-dried meat powder.
  4. 4. The pet oral deodorization and teeth strengthening biological agent according to claim 2, wherein the preparation method of the time sequence coupling symbiotic fermentation stock solution comprises the following steps: Fermenting and culturing with Bacillus licheniformis as the precursor strain, monitoring the optical density OD 600 of the fermentation liquid, inoculating Bacillus subtilis for symbiotic fermentation when the OD 600 reaches 0.60-0.90, and stopping fermentation when the spore formation rate reaches more than 90%.
  5. 5. The biological agent for deodorizing and strengthening teeth in pet oral cavity according to claim 3, wherein the modified persimmon tannin extract and the modified honeysuckle extract are prepared by the following method: the modified persimmon tannin extract is prepared by subjecting green persimmon pulp to pectase enzymolysis, enzyme deactivation and centrifugation, and collecting supernatant, adsorbing with macroporous resin, and eluting with ethanol; And (3) modifying the honeysuckle extract, namely inoculating the supernatant of the sequential coupling symbiotic fermentation stock solution into the honeysuckle alcohol extraction concentrate, performing bioconversion treatment at 30 ℃, reducing the phenolic acid content and inactivating the phenolic acid content.
  6. 6. A method for preparing the biological agent for deodorizing and strengthening teeth in the oral cavity of a pet according to any one of claims 1 to 9, comprising the steps of: S1, preparing a microbial active component, namely preparing a time sequence coupling symbiotic fermentation raw liquid, and further preparing composite probiotic dry powder by spray drying the time sequence coupling symbiotic fermentation raw liquid; S2, preparing plant extract components, namely respectively preparing a modified persimmon tannin extract and a modified honeysuckle extract, and further preparing dry powder by spray drying the modified persimmon tannin extract and the modified honeysuckle extract; s3, mixing and molding, namely mixing the microbial active component, the plant extract component, the isomaltooligosaccharide and the carrier matrix in proportion to prepare the biological preparation.
  7. 7. The method for preparing the biological agent for deodorizing and strengthening teeth in the oral cavity of the pet according to claim 6, wherein the biological agent is a spray, and the specific operation of step S3 is as follows: Adding a carrier matrix into a liquid preparation tank, and starting stirring at 120-130rpm; Sequentially adding the modified persimmon tannin extract, the modified honeysuckle extract and the isomaltooligosaccharide, and uniformly dispersing; Reducing the rotating speed to 70-80rpm, adding the time sequence coupling symbiotic fermentation stock solution, adjusting the pH value to 6.2-6.5, filtering and filling.
  8. 8. The method for preparing the biological agent for deodorizing and strengthening teeth in the oral cavity of the pet according to claim 6, wherein the biological agent is tooth cleaning powder, and the specific operation of the step S3 is as follows: premixing the carrier matrix with isomaltooligosaccharide at a mixing speed of 20-22rpm; Sequentially adding the compound probiotic dry powder, the modified persimmon tannin extract dry powder and the modified honeysuckle extract dry powder by adopting an equivalent incremental method; and (3) under the environment of room temperature and relative humidity of less than 45%, regulating the rotating speed to 25-28rpm, and continuously mixing until the color is uniform.
  9. 9. The method for preparing the biological agent for deodorizing and strengthening teeth in the oral cavity of the pet according to claim 6, wherein in the step S1, the spray drying condition of the compound probiotic dry powder is as follows: maltodextrin is added into the time sequence coupling symbiotic fermentation stock solution, the air inlet temperature is controlled to be 150-160 ℃, and the air outlet temperature is controlled to be 65-70 ℃.
  10. 10. The method for preparing the pet oral cavity deodorizing and teeth-strengthening biological agent according to claim 6, wherein in the step S1, in the preparation process of the time sequence coupling symbiotic fermentation stock solution, the fermentation temperature of bacillus licheniformis is 36-38 ℃, and after bacillus subtilis is inoculated, the fermentation temperature is adjusted to 35-36.5 ℃ and the rotation speed is increased to 220-250rpm.

Description

Biological agent for deodorizing and tooth strengthening of pet oral cavity and preparation method thereof Technical Field The invention relates to the field of daily necessities and biotechnology of pets, in particular to a biological agent for deodorizing and fixing teeth in the oral cavity of a pet and a preparation method thereof. Background With the popularization of domestic pet feeding, the oral health problem of the pets is increasingly remarkable, and dental plaque accumulation, dental calculus formation and halitosis and periodontal diseases caused by the dental plaque accumulation and the dental plaque formation are common oral puzzles of the pets. Not only affects the eating and quality of life of pets, pathogenic bacteria in the oral cavity can induce systemic diseases through blood circulation. The present pet oral cleaning products on the market mostly depend on chemical bactericides or antibiotic ingredients, such as adding chlorhexidine, triclosan or high concentration alcohol, etc. The chemical preparation can inhibit oral bacteria in a short time and mask peculiar smell, but can easily stimulate fragile oral mucosa of pets to cause biological discomfort after long-term use, and meanwhile, the broad-spectrum bactericide can indiscriminately kill normal flora in the oral cavity to cause micro-ecological unbalance of the oral cavity, and once the chemical preparation is used, odor-causing bacteria and pathogenic bacteria often rebound rapidly to cause repeated halitosis. In recent years, the use of probiotics to regulate oral cavity micro-ecology has become a new direction for pet care. However, in the existing oral probiotic preparation for pets, probiotics are sensitive to water activity, osmotic pressure and oxidation environment in a liquid system, and the survival rate of the strain in the shelf life is difficult to ensure by a conventional physical mixing process, so that the viable count of the product is reduced when the product is used, and the product cannot be effectively planted. In addition, in order to pursue a rapid deodorizing effect, plant extracts such as tannin-rich plant materials are often formulated in the preparation. However, natural plant polyphenol has stronger protein precipitation, is easy to combine with probiotic cell membrane proteins to flocculate, generates antagonism effect, and limits the efficacy of a probiotic and plant extract compound system. In addition, the existing products mostly adopt single bacterial strains or simple bacterial powder for compounding, lack of regulation and control on fermentation metabolic processes, and are difficult to induce the bacterial strains to generate enough extracellular enzyme systems such as glucanase and the like, so that the cleaning capability of the bacterial strains on refractory dental plaque biological membranes is insufficient. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a biological agent for deodorizing and fixing teeth in the oral cavity of a pet and a preparation method thereof, and solves the problems that probiotics in the existing preparation for the oral cavity of the pet have low survival rate in a liquid environment, are easy to flocculate and antagonize with plant polyphenol deodorizing components and have strong irritation to the mucous membrane of the oral cavity. The invention aims at realizing the aim by adopting the technical scheme that the biological agent for deodorizing and fixing teeth in the oral cavity of the pet and the preparation method thereof. In a first aspect, the invention provides a biological agent for deodorizing and fixing teeth in the oral cavity of a pet, which adopts the following technical scheme: A biological agent for deodorizing and strengthening teeth of pet oral cavity is prepared from the following raw materials in percentage by weight: 18.0% -50.0% of microbial active component; 4.0% -12.0% of modified persimmon tannin extract; 2.0% -5.0% of modified honeysuckle extract; 2.0% -6.0% of isomaltooligosaccharide; The balance being a carrier matrix; The microbial active components are time sequence coupling symbiotic fermentation stock solution and composite probiotic dry powder prepared from the time sequence coupling symbiotic fermentation stock solution; The modified persimmon tannin extract is a modified persimmon tannin extract and dry powder prepared from the modified persimmon tannin extract; the modified honeysuckle extract is modified honeysuckle extract and dry powder prepared from the modified honeysuckle extract. By adopting the technical scheme, the invention can obtain the following beneficial effects: The microbial stress resistance is improved, and the time sequence coupling symbiotic fermentation stock solution is adopted as an active core. In the fermentation process, the lead strain (bacillus licheniformis) preferentially utilizes a quick-acting carbon source in a culture medium and consumes dissolved oxygen