CN-121987549-A - Immune cell supernatant application and preparation method thereof

Abstract

The invention relates to a method for separating immune cells through human peripheral blood, obtaining supernatant of byproducts in the process of culturing immune cells in a GMP laboratory, taking a basal liquid and dressing mode as skin external carrier as one of main components of finished products according to the prior known scientific technology, biological natural law and special efficacy, and applying the basal liquid and dressing mode to the skin surface, thereby obtaining a biological product technical method which is used for introducing various cell bioactive components from the supernatant of immune cells and acting on the skin surface to deep layers, promoting skin cells to activate and moisten, regenerating and repairing and delaying physiological aging, has obvious multi-efficacy and can be used for tissue production under specific GMP production conditions.

Inventors

• WANG XIAOYING
• WANG XUELIANG

Assignees

• 北京壹迪科技有限公司
• 王学良

Dates

Publication Date

20260508

Application Date

20260316

Claims (10)

1. 1. A skin care product for improving skin dryness and discomfort caused by internal and external factors and delaying skin physiological aging, characterized in that the skin care product component comprises immune cell supernatant.
2. 2. The method according to claim 1, wherein the immune cell supernatant is obtained from human peripheral blood, and after the immune cells are mature in the expansion culture of human body, the immune cells are separated from the culture medium, and the culture medium still rich in the cell active ingredient product is the immune cell supernatant part.
3. 3. The method according to claim 1, wherein the immune cell supernatant contains cell active ingredients derived from immune cell exosome vesicles, secretion and lysate, and the main active ingredients are a mixture of epidermal growth factor, basic fibroblast growth factor, vascular endothelial growth factor, transforming growth factor, active oxygen free radicals and the like.
4. 4. The method according to claim 3, wherein the immune cell supernatant is used as a mixture, wherein the mixture is used as an active ingredient for comprehensively producing the effects of moisturizing, repairing and delaying aging of skin.
5. 5. The method according to claim 3 and 4, wherein the immune cell supernatant is prepared by combining various non-bioactive substances with different ratios, and adjuvants, and its preparation method, to promote penetration and repair of cytokines into skin.
6. 6. The method according to claim 5, wherein the liquid combination formulation is used in combination with a specific carrier form, which is convenient and suitable for different properties of apparent skin conditions and desired effects.
7. 7. The dressing form of immune cell supernatant according to claim 5 and 6, wherein the weight ratio of immune cell supernatant to base fluid is 10% or less, and the composition is applied to skin by using membrane cloth as medium, and is mainly suitable for skin moistening and repairing and delaying skin physiological aging.
8. 8. The method according to claim 5 or 6, wherein the immune cell supernatant is in the form of an emulsion or gel, and wherein the immune cell supernatant comprises 20% or less by weight of the emulsion or gel base solution, and the composition is applied topically to skin, and is suitable for topical skin care cosmetic products.
9. 9. The process according to claim 7 or 8, wherein the process is performed under GMP-standard conditions.
10. 10. According to claims 1 to 9, the implementation and final achievement of the compliance of the product with the immunocyte supernatant as a new raw material and preparation method, requires compliance with national laws, regulations and administration regulations, without affecting the rights of the prior invention of the present technical solution.

Description

Immune cell supernatant application and preparation method thereof Technical Field The invention relates to the field of biotechnology application, in particular to a cosmetic skin care product which effectively utilizes supernatant fluid and biological activity thereof generated in the immune cell culture process, combines biological natural law and scientific technology to be applied to the product, and is applied to delay skin physiological aging in a raw material component mode. Background The prior scientific technology, patent literature and the like have ascertained that the supernatant produced in the immune cell culture process is still rich in various cell growth factors and can effectively improve and promote biological mechanisms of self skin repair growth. According to known technical literature and/or published patent applications, biological agents produced by means of immune cell culture can be retrieved for use in biomedical products that address skin lesions that occur for various reasons. However, there is no prior art which discloses a method for practically applying the supernatant produced by the culture of immune cells as a raw material to skin care products and its products. It is known to those skilled in the art that the supernatant produced during the immune cell culture process is commonly treated as biowaste waste, a phenomenon well known to those skilled in the art. How to use the byproduct supernatant generated in the immune cell culture process to avoid being abandoned is a problem that the technology field needs to solve the effective utilization and the compatibility problem with other raw materials through the technological means. Because the prior art has worry about unknown risks in aspects of biosafety, medical ethics and the like and has no step forward of compliance problems for the application of immune cell supernatant, the technical method proves that the benefit brought by the method is extremely larger than the unknown risks under the rule of compliance, and the risks are acceptable in the technical field. Therefore, the invention is an innovative technical scheme. Disclosure of Invention The invention solves the technical problem of providing a combined formula containing immune cell supernatant and a method for manufacturing a make-up skin care product under the production condition conforming to GMP specification. From the prior scientific research and technical results, it is known that the supernatant produced in the immune cell culture process is still rich in various bioactive substances and is highly consistent with the bioactive substances required by skin repair and regeneration, and the action mechanism of the supernatant produced in the immune cell culture process for promoting biological growth repair accords with the natural law and is already in the prior art, so that the invention does not need to be repeated. Therefore, the technical scheme and the method of the invention need to find a technical measure capable of solving the reutilization of bioactive substances in immune cell supernatant, and the bioactive substances are uniformly combined with other compliant raw materials to form a skin care product which can realize industrial manufacture, has obvious efficacy and is practical. First, to verify that immune cell supernatants are safe and effective against human skin, the combination formulation of the present invention is also safe and effective, the following measures are implemented: in some embodiments of the invention, elderly cancer volunteers were selected and 100% immune cell supernatant was applied to leg skin. In some embodiments of the invention, volunteers were selected that exhibited significant hand-foot syndrome following chemotherapy with capecitabine and 100% immune cell supernatant was applied to their foot skin in a spray pattern. In some embodiments of the invention, 30 volunteers of different sexes and ages are organized and applied to the skin surface in the form of a cosmetic product using 10% of the immune cell supernatant in the combined formulation of the invention as one of the main raw materials. In some embodiments of the invention, the 10% immune cell supernatant in the combined formulation of the invention is used as one of the main raw materials, and is produced in an industrial production mode, and the non-selling products packaged in the minimum use units are used by giving away to professional exhibition channels and relatives and friends channels, wherein the total number of the non-selling products reaches 2000 minimum use units. In some embodiments of the invention, the whole process of experiments, verification and confirmation is effectively controlled in an industrial production process flow and quality safety control mode strictly according to GMP specifications. In some embodiments of the invention, immune cell supernatants meeting quality safety specifications are selected and obtained by contract p