CN-121987558-A - Ultrasonic response hydrogel for spinal cord injury treatment and preparation method and application thereof

CN121987558ACN 121987558 ACN121987558 ACN 121987558ACN-121987558-A

Abstract

The invention discloses an ultrasonic response hydrogel for treating spinal cord injury, a preparation method and application thereof, the ultrasound response hydrogel comprises silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel and sodium niobate piezoelectric nanoparticles blended therein. The ultrasonic response hydrogel realizes cooperative treatment of different stages of spinal cord injury through a single system, namely in an acute stage after spinal cord injury, ultrasonic activatable sodium niobate nano particles are applied to catalyze water molecules to generate hydrogen and oxygen in situ, high-penetrability hydrogen can play a role in global anti-inflammatory, oxygen can accurately relieve an anoxic microenvironment of a damaged core area, and the conductive hydrogel enters a recovery stage, and a conductive network formed by the conductive hydrogel is combined with micro-current continuously generated by piezoelectric nano particles under ultrasonic, so that bionic electric stimulation signals can be efficiently conducted, metabolism and electric activity of nerve cells can be effectively regulated, and long-term reconstruction of nerve functions is promoted.

Inventors

WANG ZHONGYANG
LIAN XIAOFENG
You Yanling
WANG DIAN
Pu Xinjie
Yang Erzhu

Assignees

上海市第六人民医院

Dates

Publication Date: 20260508
Application Date: 20260206

Claims (9)

1. An ultrasound response hydrogel for the treatment of spinal cord injury, comprising a silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel and sodium niobate piezoelectric nanoparticles blended therein.
2. The ultrasound responsive hydrogel for the treatment of spinal cord injury according to claim 1, wherein the sodium niobate piezoelectric nanoparticles have a particle size of 20nm or less and are capable of catalyzing water to produce hydrogen and oxygen under the action of ultrasound.
3. The ultrasound responsive hydrogel for the treatment of spinal cord injury according to claim 1, wherein the sodium niobate piezoelectric nanoparticles have a particle diameter of 5-10nm.
4. A method of preparing an ultrasound responsive hydrogel for the treatment of spinal cord injury according to any one of claims 1-3, comprising the steps of: Adding niobium oxide into sodium hydroxide solution to form suspension, adding polyvinylpyrrolidone with molecular weight of 10000-40000 into the suspension, uniformly mixing, carrying out hydrothermal reaction on the mixed system at 180-220 ℃ for 6-12 hours, cooling, centrifuging and washing after the reaction is finished to obtain sodium niobate nanoparticle precipitate; Step two, annealing the sodium niobate nano particles for 4 to 8 hours at the temperature of 350 to 450 ℃ to obtain sodium niobate piezoelectric nano particles; And thirdly, blending the sodium niobate piezoelectric nano particles with silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel to obtain the ultrasonic response hydrogel.
5. The method according to claim 4, wherein the polyvinylpyrrolidone has a molecular weight of 10000 to 20000.
6. The process of claim 4, wherein the hydrothermal reaction is carried out at 200 ℃ for 8 hours.
7. The method according to claim 4, wherein the annealing treatment is performed at 400 ℃ for 6 hours.
8. The method according to claim 4, wherein the silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel is prepared by preparing a hydrogel skeleton of polyvinyl alcohol and carboxymethyl cellulose, and blending the silk fibroin solution with the polyvinyl alcohol/carboxymethyl cellulose hydrogel.
9. Use of an ultrasound responsive hydrogel as claimed in any one of claims 1-3 in the manufacture of a product for the treatment of spinal cord injury.

Description

Ultrasonic response hydrogel for spinal cord injury treatment and preparation method and application thereof Technical Field The invention relates to the technical field of biological medicines, in particular to ultrasonic response hydrogel for spinal cord injury treatment, and a preparation method and application thereof. Background Currently, clinical treatment strategies for spinal cord injury mainly include pharmaceutical intervention and physical rehabilitation therapy. Drug therapy mostly reduces secondary damage by anti-inflammatory or neuroprotective agents, while physical therapy aims to promote functional recovery. However, the existing means are difficult to accurately regulate and control the pathological microenvironment of the damaged part, and particularly cannot effectively solve the key problems of oxidative stress, tissue hypoxia and the like caused by iron death/copper death, so that the repairing effect is limited. In recent years, research shows that hydrogen is used as a selective antioxidant, can effectively neutralize strong toxic active oxygen such as hydroxyl free radicals and the like, lighten oxidative damage and inflammatory reaction, and the oxygen can improve the anoxic state of a damaged area and support cell energy metabolism and tissue repair. However, the existing treatment method cannot realize the controllable and continuous generation of hydrogen and oxygen in the spinal cord injury microenvironment, so that the synergistic effect of the hydrogen and the oxygen in the neuroprotection and regeneration is difficult to fully play. Therefore, a novel catalytic medical material which can generate hydrogen and oxygen in situ at a damaged part and respond to micro-environment abnormality is developed, and has important clinical value and application prospect. The existing spinal cord injury treatment strategy has obvious staged fracturing, wherein in the early inflammatory stage of injury, the clinic mainly adopts high-dose hormone anti-inflammatory treatment, and in the recovery stage, the nerve function remodeling means such as electric stimulation, rehabilitation training and the like are turned. This staged mode of treatment results in lack of continuity of intervention and failure to produce a synergistic effect. Early anti-inflammatory treatment fails to create a favorable microenvironment basis for later nerve regeneration, and later rehabilitation means are difficult to reverse oxidative damage and metabolic disorders accumulated in the early stage. More importantly, the prior art has not yet achieved an intelligent therapeutic system that can be dynamically based on different pathological stages of spinal cord injury. For example, traditional materials fail to achieve further stimulation to promote regeneration of nerve axons during recovery while being anti-inflammatory in the acute phase. This separation of function severely limits the overall repair effect of spinal cord injury. Therefore, developing a therapeutic platform with staged regulation and control capability and multi-mechanism cooperation becomes an urgent need in the field of current spinal cord injury repair. Disclosure of Invention The invention aims at overcoming the defects in the prior art and provides ultrasonic response hydrogel for treating spinal cord injury, and a preparation method and application thereof. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: A first aspect is to provide an ultrasound responsive hydrogel for the treatment of spinal cord injury comprising a silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel and sodium niobate piezoelectric nanoparticles blended therein. Further, the particle size of the sodium niobate piezoelectric nanoparticle is below 20nm, and the sodium niobate piezoelectric nanoparticle can catalyze water to generate hydrogen and oxygen under the action of ultrasound. Further, the particle size of the sodium niobate piezoelectric nanoparticle is 5-10nm. The second aspect provides a method for preparing the ultrasonic response hydrogel for treating spinal cord injury, which comprises the following steps: Adding niobium oxide into sodium hydroxide solution to form suspension, adding polyvinylpyrrolidone with molecular weight of 10000-40000 into the suspension, uniformly mixing, carrying out hydrothermal reaction on the mixed system at 180-220 ℃ for 6-12 hours, cooling, centrifuging and washing after the reaction is finished to obtain sodium niobate nanoparticle precipitate; Step two, annealing the sodium niobate nano particles for 4 to 8 hours at the temperature of 350 to 450 ℃ to obtain sodium niobate piezoelectric nano particles; And thirdly, blending the sodium niobate piezoelectric nano particles with silk fibroin/polyvinyl alcohol/carboxymethyl cellulose hydrogel to obtain the ultrasonic response hydrogel. Further, the molecular weight of the polyvinylpyrrolidone is 10000-20000. Further, the hy