Search

CN-121987569-A - Preparation method of exosome-carrying Vaseline hydrogel composite emulsion

CN121987569ACN 121987569 ACN121987569 ACN 121987569ACN-121987569-A

Abstract

The invention belongs to the technical field of exosomes, and discloses a preparation method of a vaseline hydrogel composite emulsion carrying exosomes, which comprises the following steps of preparing raw materials, preparing medical vaseline, sodium alginate, an emulsifying agent (Tween 80), glycerol, an antioxidant (vitamin E) and exosomes, and performing quality inspection on all the raw materials to ensure no impurity and deterioration; and step two, melting Vaseline. The Vaseline hydrogel composite emulsion disclosed by the invention fuses Vaseline and hydrogel, overcomes the defect of a single carrier, has good air permeability and moisture retention sealing property, can stably release exosomes, improves the skin absorption efficiency, effectively protects a wound surface, has strong stability, can be stored for a long time, and optimally reduces the damage to exosomes in the preparation process, avoids the damage to the activity caused by high-temperature high-shear, ensures the exosomes activity, improves the healing effect on wounds, and provides a better scheme for wound treatment.

Inventors

  • LIN YAN

Assignees

  • 林燕

Dates

Publication Date
20260508
Application Date
20260319

Claims (9)

  1. 1. The preparation method of the exosome-carrying Vaseline hydrogel composite emulsion is characterized by comprising the following specific steps: Step one, raw material preparation Preparing medical vaseline, sodium alginate, an emulsifier (Tween 80), glycerol, an antioxidant (vitamin E) and exosomes, and performing quality inspection on all raw materials to ensure no impurity or deterioration; step two, melting vaseline Weighing a proper amount of medical vaseline according to the formula proportion, placing the container into a water bath kettle, setting the water bath temperature to be 80-90 ℃, and slowly stirring the vaseline by using a stirrer in the heating process to ensure that the vaseline is heated uniformly until the vaseline is completely dissolved; Step three, preparing hydrogel phase According to the formula proportion, weighing a corresponding amount of sodium alginate, slowly adding the sodium alginate into pure water, stirring while adding to promote dissolution, adding a specified amount of emulsifying agent and 5% of glycerol after complete dissolution, continuously stirring to uniformly mix the emulsifying agent and the glycerol, then placing the mixed solution into heating equipment, heating to 85 ℃, and keeping the temperature for 10-15 minutes to ensure that all the components are fully fused; step four, emulsifying and mixing After the vaseline is completely dissolved and the hydrogel phase is heated to 85 ℃, slowly pouring the hydrogel phase into a container filled with the vaseline, and then stirring the mixed solution by using a high-speed stirrer, and closely observing the state of the mixed solution in the stirring process until the mixed solution forms a uniform milky cream shape; step five, cooling treatment After stopping stirring, taking the container filled with the emulsified mixture out of the heating equipment, and naturally cooling in a room temperature environment until the temperature is reduced to 40-45 ℃; Step six, adding antioxidant and exosome Accurately weighing 0.5% of antioxidant and exosomes with required concentration according to the formula proportion, slowly adding the antioxidant into the mixed solution cooled to 40-45 ℃, slightly stirring to primarily mix the antioxidant, and then slowly adding the exosomes into the mixed solution to avoid mechanical damage to the exosomes; Step seven, stirring gently The mixed solution is lightly stirred by using a stirrer at a low rotating speed, so that the antioxidant and exosome are ensured to be uniformly dispersed in the mixed solution, and a uniform system is formed; Step eight, filling and solidifying Selecting a clean and sterile container, slowly pouring the stirred composite emulsion into the container, sealing the container after filling, and naturally solidifying the container in a room temperature environment.
  2. 2. The preparation method of the exosome-carrying Vaseline hydrogel composite emulsion according to claim 1, wherein the medical Vaseline, sodium alginate, emulsifier, glycerol and antioxidant in the first step are 70-80% by weight, 10-20% by weight, 1-3% by weight, 5% by weight and 0.5% by weight.
  3. 3. The method for preparing the exosome-carrying Vaseline hydrogel composite emulsion according to claim 1, wherein the container in the second step is placed in a water bath kettle, the container is required to be completely immersed in water, but water cannot overflow, meanwhile, the water level of the water bath kettle is closely observed in the heating process, and hot water is timely added.
  4. 4. The method of claim 1, wherein deionized water or distilled water is used as the pure water in the third step, and sodium alginate is slowly added into the pure water and stirred continuously.
  5. 5. The preparation method of the exosome-carrying Vaseline hydrogel composite emulsion according to claim 1, wherein the preparation method is characterized in that when the hydrogel phase is slowly poured into a container filled with Vaseline in the step four, a dropper or a funnel is used for controlling the pouring speed, so that the hydrogel phase is uniformly distributed in the Vaseline, and when the high-speed stirrer is used for stirring the mixed solution in the step four, the rotating speed is set at 2500-3500rpm, and the stirring time is 50-70 minutes.
  6. 6. The method of claim 1, wherein the step five is performed in a room temperature environment for natural cooling, and a thermometer is used to monitor the temperature of the mixture in real time, and the thermometer is inserted into the center of the mixture to ensure accurate measurement.
  7. 7. The method of claim 1, wherein the step six of slowly adding the antioxidant into the mixture cooled to 40-45 ℃ requires gentle stirring to enable the antioxidant to be rapidly dispersed in the mixture.
  8. 8. The method of claim 1, wherein the rotational speed of the agitator in the seventh step is controlled to be 500-1000rpm, and the agitation time is 5-10 minutes.
  9. 9. The method for preparing the exosome-carrying Vaseline hydrogel composite emulsion according to claim 1, wherein the composite emulsion is subjected to stability tests after preparation, and specifically comprises sensory and appearance stability tests, centrifugal stability tests, viscosity and rheology tests, exosome exclusive tests, activity retention tests and accelerated aging tests.

Description

Preparation method of exosome-carrying Vaseline hydrogel composite emulsion Technical Field The invention belongs to the technical field of exosomes, and particularly relates to a preparation method of an exosome-carrying Vaseline hydrogel composite emulsion. Background The exosomes are nanoscale vesicles secreted by cells, have the diameter of about 30-150 nanometers, have a lipid bilayer membrane structure, contain bioactive substances such as proteins, lipids, RNA, DNA and the like, widely exist in body fluids such as blood, saliva, urine and the like, are used as messengers for intercellular communication, can regulate the functions of receptor cells, and participate in the processes such as immune response, tissue repair and the like. When using the exosome to cure the wound, the vaseline ointment or the pure water gel is usually used as a carrier, but has obvious defects that the vaseline ointment has poor air permeability, slow permeation, difficult exosome release and low skin absorption efficiency, the pure water gel has weak moisture retention and sealing property, is easy to dry and fall off, has insufficient protection on the wound surface, is easy to deactivate the exosome, is easy to laminate, oil separation and caking due to simple physical mixing, has poor stability, is difficult to store for a long time, and has the defect that most of formulas are not optimized for the exosome, and the high temperature and high shearing are easy to cause cracking and activity loss of the exosome, so the wound treatment effect is influenced, and the improvement is needed. Disclosure of Invention The invention aims to provide a preparation method of an exosome-carrying Vaseline hydrogel composite emulsion, which aims to solve the problems in the background technology. In order to achieve the aim, the invention provides the following technical scheme that the preparation method of the exosome-carrying Vaseline hydrogel composite emulsion comprises the following specific steps: Step one, raw material preparation Preparing medical vaseline, sodium alginate, an emulsifier (Tween 80), glycerol, an antioxidant (vitamin E) and exosomes, and performing quality inspection on all raw materials to ensure no impurity or deterioration; step two, melting vaseline Weighing a proper amount of medical vaseline according to the formula proportion, placing the container into a water bath kettle, setting the water bath temperature to be 80-90 ℃, and slowly stirring the vaseline by using a stirrer in the heating process to ensure that the vaseline is heated uniformly until the vaseline is completely dissolved; Step three, preparing hydrogel phase According to the formula proportion, weighing a corresponding amount of sodium alginate, slowly adding the sodium alginate into pure water, stirring while adding to promote dissolution, adding a specified amount of emulsifying agent and 5% of glycerol after complete dissolution, continuously stirring to uniformly mix the emulsifying agent and the glycerol, then placing the mixed solution into heating equipment, heating to 85 ℃, and keeping the temperature for 10-15 minutes to ensure that all the components are fully fused; step four, emulsifying and mixing After the vaseline is completely dissolved and the hydrogel phase is heated to 85 ℃, slowly pouring the hydrogel phase into a container filled with the vaseline, and then stirring the mixed solution by using a high-speed stirrer, and closely observing the state of the mixed solution in the stirring process until the mixed solution forms a uniform milky cream shape; step five, cooling treatment After stopping stirring, taking the container filled with the emulsified mixture out of the heating equipment, and naturally cooling in a room temperature environment until the temperature is reduced to 40-45 ℃; Step six, adding antioxidant and exosome Accurately weighing 0.5% of antioxidant and exosomes with required concentration according to the formula proportion, slowly adding the antioxidant into the mixed solution cooled to 40-45 ℃, slightly stirring to primarily mix the antioxidant, and then slowly adding the exosomes into the mixed solution to avoid mechanical damage to the exosomes; Step seven, stirring gently The mixed solution is lightly stirred by using a stirrer at a low rotating speed, so that the antioxidant and exosome are ensured to be uniformly dispersed in the mixed solution, and a uniform system is formed; Step eight, filling and solidifying Selecting a clean and sterile container, slowly pouring the stirred composite emulsion into the container, sealing the container after filling, and naturally solidifying the container in a room temperature environment. As a preferable technical scheme of the invention, the medical vaseline, sodium alginate, emulsifier, glycerin and antioxidant in the first step are 70-80% by weight of medical vaseline, 10-20% by weight of sodium alginate, 1-3% by weight of emulsifier, 5% by weight of gly