CN-121987592-A - Self-assembled naringenin-loaded silk fibroin nanoparticle and preparation method and application thereof

Abstract

The invention relates to the technical field of biology, in particular to a self-assembled naringenin-loaded silk fibroin nanoparticle, and a preparation method and application thereof. The preparation method comprises the steps of mixing a silk fibroin aqueous solution and a naringenin ethanol solution, and performing ultrasonic treatment to obtain the silk fibroin nano particles. The self-assembled naringenin-loaded silk fibroin nanoparticle prepared by the preparation method provided by the invention has the advantages of improving the solubility and dispersibility of naringenin, enhancing the stability of naringenin, improving the bioavailability, promoting the application and expansion of a nanometer delivery system, improving the uniformity of nanoparticles and the like.

Inventors

• LIU KAI
• ZHANG LIYUAN
• CHEN JIAYU
• YU RUNZHONG

Assignees

• 黑龙江八一农垦大学

Dates

Publication Date

20260508

Application Date

20260320

Claims (9)

1. 1. A preparation method of self-assembled naringenin-loaded silk fibroin nanoparticles is characterized by comprising the steps of mixing a silk fibroin aqueous solution and naringenin ethanol solution, and performing ultrasonic treatment to obtain the silk fibroin nanoparticles.
2. 2. The method according to claim 1, wherein the concentration of the silk fibroin in the silk fibroin aqueous solution is 20 mg mL -1 .
3. 3. The preparation method of claim 1, wherein the mass ratio of the silk fibroin to the naringenin in the mixed solution obtained by mixing is 40:1-60:1.
4. 4. The method of claim 1, wherein the ultrasound has a power of 90W for a time of 6 min.
5. 5. Silk fibroin nanoparticles prepared by the method of any one of claims 1-4.
6. 6. Use of the silk fibroin nanoparticles of claim 5 in the preparation of an antioxidant product.
7. 7. An antioxidant product, characterized in that it comprises the silk fibroin nanoparticles of claim 5.
8. 8. The antioxidant product of claim 7, wherein the product further comprises an adjunct.
9. 9. The antioxidant product of claim 8, wherein the dosage form of the product comprises a granule, powder, or capsule.

Description

Self-assembled naringenin-loaded silk fibroin nanoparticle and preparation method and application thereof Technical Field The invention relates to the technical field of biology, in particular to a self-assembled naringenin-loaded silk fibroin nanoparticle, and a preparation method and application thereof. Background Naringenin is a natural active ingredient, and has various biological activities such as antioxidation, anti-inflammatory and anticancer, so that naringenin has wide application in the fields of medicine, food and cosmetics. Naringenin, however, suffers from a number of problems in practical use, such as poor water solubility, poor stability, low bioavailability, etc. Therefore, the construction of the nano-encapsulation system has important significance for improving the bioavailability of naringenin. Naringenin belongs to hydrophobic molecules, has extremely low solubility in aqueous phase environment, severely limits the bioavailability and the preparation stability, is sensitive to light, heat and pH change, is easy to degrade in the processing and storage processes, and influences the durability of the efficacy. The nanometer delivery system is characterized in that a biocompatible carrier material is utilized to encapsulate the drug components through physical adsorption or chemical covalent bonding means, and after the drug components are directionally delivered to a region, the accurate regulation and control of delivery are realized through the material characteristics or an environmental response mechanism, and slow release and controllable release are realized. Common nano-systems comprise liposome, polymer nanoparticles, dendritic macromolecules, carbon nanotubes, quantum dots, metal nanoparticles and the like, and the nano-systems are widely applied to a plurality of treatment fields such as anticancer, anti-inflammatory, anti-infection, vaccine development and the like by enhancing the stability of the medicine, realizing controllable release and crossing biological barriers. Although nano-drugs have been successfully applied to clinical and food fields, the development of nano-drugs still faces the problems of low drug loading rate, unstable encapsulation, easy leakage, nonuniform particle size, easy aggregation and the like. Thus, there is a need to find a method that can stably encapsulate naringenin. Disclosure of Invention The invention aims to provide self-assembled naringenin-loaded silk fibroin nanoparticles, and a preparation method and application thereof, so as to solve the problems in the prior art. In order to achieve the above object, the present invention provides the following solutions: The invention provides a preparation method of self-assembled naringenin-loaded silk fibroin nanoparticles, which comprises the steps of mixing a silk fibroin aqueous solution and naringenin ethanol solution, and performing ultrasonic treatment to obtain the silk fibroin nanoparticles. Optionally, the concentration of the silk fibroin in the silk fibroin aqueous solution is 20 mg-mL -1. Optionally, in the mixed solution obtained by mixing, the mass ratio of the silk fibroin to the naringenin is 40:1-60:1. Optionally, the power of the ultrasound is 90W and the time is 6 min. The invention provides the silk fibroin nanoparticle prepared by the preparation method. The invention provides application of the silk fibroin nano particles in preparation of antioxidant products. The invention provides an antioxidant product, which comprises the silk fibroin nanoparticle. Optionally, the product further comprises an auxiliary material. Optionally, the dosage form of the product comprises granules, powder or capsules. The invention discloses the following technical effects: Silk fibroin mainly contains two crystalline states, I-type and II-type secondary structures mainly comprise alpha helices and non-beta-sheets, the structural properties are unstable and easily soluble in water, and silk II mainly comprises beta-sheets and is stable and insoluble in water. Silk fibroin can undergo transformation from its type I structural hydrogen bonding surface to type II structure under specific environmental conditions such as pH, temperature, various ions, alcohol reagents, shearing or stretching forces, etc. Silk fibroin can therefore be used as a carrier for developing delivery systems for stable functional ingredients. The invention constructs a silk fibroin nanometer delivery system by utilizing the transformation of the silk fibroin structure, and provides a method for encapsulating naringenin. Meanwhile, the invention has the following advantages: 1. Improving the solubility and dispersibility of naringenin The characteristic that the silk fibroin can form a stable nano structure under a specific condition is utilized to encapsulate the hydrophobic naringenin, so that the solubility and the dispersibility of the naringenin in an aqueous phase environment are improved, and the defect of poor water