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CN-121987594-A - Nanometer medicine carrying granule based on gamma-polyglutamic acid and epsilon-polylysine and preparation method and application thereof

CN121987594ACN 121987594 ACN121987594 ACN 121987594ACN-121987594-A

Abstract

The invention provides a nano medicine carrying particle based on gamma-polyglutamic acid and epsilon-polylysine, wherein a composite shell is arranged outside the nano medicine carrying particle, the composite shell is formed by the reaction of the gamma-polyglutamic acid and the epsilon-polylysine, a single active ingredient is contained in the composite shell, and the single active ingredient is one of salicylic acid, gallic acid or tea polyphenol. The invention also provides a preparation method and application of the nano drug-loaded particles. The nanoparticle adopts a uniform ion crosslinking process to respectively prepare three nanoparticles with gamma-polyglutamic acid and epsilon-polylysine serving as composite shells and cores for loading different single active ingredients. The nano-particles provided by the invention have stable structure and good biocompatibility, and can be used for preparing medicines with anti-aging, antibacterial and anti-inflammatory effects respectively or in combination.

Inventors

  • Qiao changcheng
  • Bao Yanbin
  • GAI LIFENG
  • LI ZHENHAI
  • ZHONG HAIJIAO

Assignees

  • 天津科技大学
  • 天津北洋百川生物技术有限公司

Dates

Publication Date
20260508
Application Date
20260410

Claims (9)

  1. 1. The nano medicine carrying granule based on gamma-polyglutamic acid and epsilon-polylysine is characterized in that a composite shell is arranged outside the nano medicine carrying granule, the composite shell is formed by the reaction of gamma-polyglutamic acid and epsilon-polylysine, a single active ingredient is contained in the composite shell, and the single active ingredient is one of salicylic acid, gallic acid or tea polyphenol.
  2. 2. The nano drug-loaded particle according to claim 1, wherein the mass ratio of gamma-polyglutamic acid to epsilon-polylysine is 1:10-15.
  3. 3. The composition of nano-drug-loaded particles based on gamma-polyglutamic acid and epsilon-polylysine according to claim 1 wherein the composition comprises three nano-drug-loaded particles, wherein the three nano-drug-loaded particles are nano-drug-loaded particles of salicylic acid, gallic acid and tea polyphenol as the entrapped single active ingredients in claim 1, respectively.
  4. 4. The method for preparing the nano-drug-loaded particles according to claim 1, comprising the steps of: Step 1, respectively dissolving epsilon-polylysine and gamma-polyglutamic acid in deionized water to obtain epsilon-polylysine solution and gamma-polyglutamic acid solution; Step 2, adding any one single active ingredient of salicylic acid, gallic acid or tea polyphenol into the epsilon-polylysine solution, and stirring for dissolution to obtain epsilon-polylysine mixed solution containing the single active ingredient; Step 3, dropwise adding the gamma-polyglutamic acid solution into the mixed solution obtained in the step 2 under the stirring condition for reaction; And 4, after the reaction is finished, centrifuging the reaction solution, collecting precipitate, and freeze-drying to obtain nano drug-carrying particles loaded with corresponding single active ingredients.
  5. 5. The method according to claim 4, wherein the dropping rate of the gamma-polyglutamic acid solution in the step 3 is 0.2-1.0 mL/min.
  6. 6. The method according to claim 4, wherein the stirring speed in the step 3 is 400 to 600 rpm.
  7. 7. The method according to claim 4, wherein the reaction temperature in step 3 is 20 to 30 ℃ and the reaction time is 20 to 40 minutes.
  8. 8. The method according to claim 4, wherein the centrifugation conditions in the step 4 are centrifugation at 10000 to 15000 rpm at 2 to 8℃for 20 to 40 minutes.
  9. 9. Use of the nano-drug-loaded particles according to claim 1 or the composition of nano-drug-loaded particles according to claim 3 for the preparation of a medicament for skin anti-aging, antibacterial, anti-inflammatory or acne treatment.

Description

Nanometer medicine carrying granule based on gamma-polyglutamic acid and epsilon-polylysine and preparation method and application thereof Technical Field The invention belongs to the technical field of drug-loaded nano-particles, and particularly relates to a gamma-polyglutamic acid/epsilon-polylysine-based series of nano drug-loaded particles, and a preparation method and application thereof. Background Skin aging and microbial infection are two core problems affecting skin health, and development of external medicines with anti-aging and antibacterial effects has become an important research direction in the field of pharmacy. Natural active ingredients such as salicylic acid (having keratolytic and anti-inflammatory effects), gallic acid (potent antioxidant) and tea polyphenols (broad-spectrum antibacterial and anti-photoaging) show great potential for use in such drugs due to their good biological activity and safety. However, these active ingredients face a series of common challenges in practical applications: (1) Poor stability, e.g., tea polyphenols and gallic acid are susceptible to oxidative inactivation, salicylic acid is sensitive to light; (2) The dissolubility and the transdermal property are limited, namely, partial components are poorly water-soluble or fat-soluble and are difficult to effectively permeate to the target part of the skin; (3) Irritation, namely, high concentration salicylic acid and other components can irritate skin, and influence the use experience and compliance. These problems severely restrict the full play and commercial application of the efficacy thereof. To overcome the above drawbacks, nanodrug delivery techniques are widely introduced. Among them, natural polyelectrolyte-based nanocarriers, such as gamma-polyglutamic acid (gamma-PGA) and epsilon-polylysine (epsilon-PLL), are attracting attention due to their excellent biocompatibility, degradability, and mild preparation conditions. gamma-PGA/epsilon-PLL composite nanoparticles constructed by ionic cross-linking have proven to be an effective drug delivery system. In the prior art, there have been studies utilizing such carriers to carry a single drug (e.g., an antibiotic or a single antioxidant), but their use has focused on a single therapeutic purpose (e.g., antibacterial only or chemotherapeutic drug only delivery). At present, the field still has obvious defects that firstly, a common co-loading strategy aiming at various functional active ingredients is lacked, particularly for synchronous high-efficiency loading of anti-aging and antibacterial ingredients with large differences in physicochemical properties, secondly, the existing scheme focuses on single-functional or single-ingredient delivery, a platform type nano carrier system capable of flexibly loading different functional active ingredients and realizing 'anti-aging-antibacterial' synergistic effect cannot be systematically constructed, and finally, in terms of technology, how to realize high-efficiency encapsulation of different ingredients through a simple and controllable single process, ensure stability and reproducibility of nano particles, and still the technical problem to be solved is solved. Therefore, there is an urgent need to develop a novel nano drug-carrying system, which can encapsulate different types of active ingredients respectively by a uniform and efficient preparation method to form serial nano particles with stable structure and good biocompatibility, so as to meet the requirements of precise, mild and cooperative delivery of various active ingredients in skin multi-effect nursing and treatment. Disclosure of Invention The invention aims to overcome the defects in the prior art, and aims to provide a gamma-polyglutamic acid/epsilon-polylysine-based series nano drug-loaded particle and a preparation method thereof, aiming at the problem that a platform-type nano drug-loaded system which can be used for respectively and efficiently encapsulating various functional active ingredients and has uniform process is lacking in the prior art. The invention aims to solve the problems that a single carrier is difficult to load active ingredients with different properties universally and the prior art focuses on single efficacy, and provides a nano delivery platform which can realize flexible and stable loading of anti-aging and antibacterial active ingredients and has good biocompatibility. To achieve the above object, in a first aspect, the present invention provides a gamma-polyglutamic acid/epsilon-polylysine-based nanoparticle for drug delivery. The nano particles are crosslinked with epsilon-polylysine (epsilon-PLL) acid through gamma-polyglutamic acid (gamma-PGA) ions to form a composite shell, and a single active ingredient is contained in the composite shell and is any one of salicylic acid, gallic acid or tea polyphenol. Preferably, the mass ratio of the gamma-polyglutamic acid to the epsilon-polylysine is 1:10-15. Preferably, an