CN-121987611-A - Construction method of sensitive skin animal model

Abstract

The invention discloses a construction method of a sensitive skin animal model, and belongs to the technical field of animal model construction. According to the invention, the sensitive skin animal model is constructed by smearing sodium dodecyl sulfate and veratrine or capsaicin on the back skin of a mouse after dehairing, and the method can simulate not only physiological indexes of sensitive skin of a human, but also key pathological processes of sensitive skin of the human by detecting key pathological indexes such as damaged skin barrier, nerve and blood vessel hyperreactivity, immune inflammation and the like, can simulate natural pathophysiological processes of sensitive skin of the human, basically covers pathogenesis of sensitive skin, induces barrier damage of the skin of the mouse, nerve and blood vessel hyperreactivity and inflammatory reaction, and accords with clinical practice. In addition, the method has the advantages of good repeatability, high safety, simple and convenient operation, can promote the screening of sensitive skin medicaments and the development of personalized treatment schemes, shortens the development period of sensitive skin related treatment schemes, and has good application prospect.

Inventors

• HE LI
• YANG XINWANG
• XU ZEHENG

Assignees

• 昆明医科大学

Dates

Publication Date

20260508

Application Date

20260410

Claims (5)

1. 1. The construction method of the sensitive skin animal model is characterized by comprising the following steps of: 1) Female BALB/c mice of 6-8 weeks were kept for 2-3 days and dehaired; 2) After the mice are subjected to adaptive feeding for 2 days after dehairing, uniformly smearing sodium dodecyl sulfate on the skin of all backs, wherein the interval time of each time is 12-16h, and the continuous smearing is carried out for 4-9 days; 3) Coating veratric or capsaicin for 6-8 hours after coating the sodium dodecyl sulfate for the first time every day from the 3 rd day of coating the sodium dodecyl sulfate in the step 2), and continuously coating for 4 days 1 time every day; mice obtained a sensitive skin animal model after day 7 after the initial application of sodium dodecyl sulfate.
2. 2. The method according to claim 1, wherein in step 2), the concentration of sodium dodecyl sulfate is 1-5% and the application volume is 10-100. Mu.L.
3. 3. The method according to claim 1, wherein in step 3), veratrine is at a concentration of 10-200 μΜ and the smear volume is 10-100 μΜ.
4. 4. The method according to claim 1, wherein in step 3), the capsaicin concentration is 1-20 μg/100 μl and the application volume is 10-100 μl.
5. 5. The method according to claim 1, wherein the application method of the medicine is to grasp the tail of the mouse with one hand, make it climb on the cage, suck the medicine liquid to be applied with a pipette, uniformly apply it on the skin of the mouse, and uniformly apply it with the finger wearing a glove.

Description

Construction method of sensitive skin animal model Technical Field The invention belongs to the technical field of animal model construction, and particularly relates to a construction method of a sensitive skin animal model. Background The sensitive skin is used as a sub-health state of the skin which is highly developed on the face, and the sensitive skin is clinically characterized in that subjective discomfort such as burning, stinging, itching, tightness and the like easily occurs under physical stimulation, chemical contact or mental pressure, and can be accompanied with objective signs such as erythema, telangiectasia and the like, so that double burden is caused on the life quality and psychological health of patients, and physical and psychological health is seriously influenced. The pathogenesis of sensitive skin at present mainly surrounds three aspects of barrier damage, neurovascular hyperreactivity and immune inflammation. The influencing factors mainly comprise frequent replacement of cosmetics or simultaneous selection of various brands of cosmetics, excessive facial cleaning, repeated use of disinfection products, external stimulation drugs, local and long-term massive external glucocorticoids and the like. Studies have shown an increase in the transepidermal water loss rate (TRANS EPIDERMAL WATER loss, TEWL) of sensitive skin, a decrease in the water content of the stratum corneum, suggesting an impaired skin barrier function. Skin barrier function is critical for maintaining skin health and depends primarily on the structural integrity of the Stratum Corneum (SC) and the tight junctions (tight junctions, TJs). When these structures are damaged, the skin protective ability is reduced, leading to more invasive external irritants and allergens, ultimately leading to sensitive skin. It was found that decreased expression of claudin 1 (claudin-1) in the epidermis resulted in defects in skin barrier function, and recent studies showed that down-regulation of claudin 5 (claudin-5) expression in sensitive skin lesions resulted in incomplete structure of the claudin (TJs) and impaired epidermal barrier function, which may be a key barrier protein for the pathogenesis of sensitive skin. Transient receptor potential vanilloid subtype 1 receptor (TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, subfamily V, membrane 1, trpv 1) is widely expressed in skin nociceptive sensory nerve endings, keratinocytes and mast cells. After the skin barrier is damaged, the protective effect on the nerve endings and blood vessels of the skin is weakened. Proved by researches, TRPV1 can induce mast cells to degranulate, promote neurotransmitter release of vasoactive substances such as intestinal polypeptide (VIP), substance P (SP), calcitonin Gene Related Polypeptide (CGRP) and the like, trigger neurogenic inflammatory reaction, promote mast cells to secrete endothelin (endothelin, ET), further induce tumor necrosis factor (TNF-alpha) and interleukin (IL-6) to secrete, promote vascular endothelial growth factor (vascular endothelial growth factor, VEGF) to generate, and lead to vascular reactivity increase and vascular dilation. The core of the established sensitive skin mouse model is to simulate the damage of skin barrier, and the method of sticking adhesive tape and combining capsaicin is commonly used in the related research at present to establish the model, but the method is easy to cause skin exudation and crusting, and a plurality of reports indicate that the capsaicin has anti-inflammatory and skin barrier repairing effects, so that the capsaicin has certain limitation and does not accord with the natural induction process of sensitive skin. More importantly, there is currently a lack of modeling methods that can comprehensively assess skin sensitivity from four major dimensions of barrier, inflammation, blood vessels, and nerves. Therefore, the invention aims to establish an animal model capable of comprehensively reflecting multidimensional pathological characteristics (including barrier injury, nerve hypersensitivity, vasodilation and immune inflammation) of sensitive skin and provides a reliable tool for sensitive skin research. Disclosure of Invention In order to solve the problems, the invention aims to provide a construction method of a sensitive skin animal model. The invention aims to realize the method for constructing the sensitive skin animal model, which comprises the following steps: 1) Female BALB/c mice of 6-8 weeks were kept for 2-3 days and dehaired; 2) After the mice are subjected to adaptive feeding for 2 days after dehairing, uniformly smearing Sodium Dodecyl Sulfate (SDS) on the skin of all backs, wherein the interval time of each time is 12-16h, and the continuous smearing is carried out for 4-9 days; 3) Coating veratric acid (VTD) or capsaicin (CAPSAICIN, CS) for 6-8 hours after the first coating of sodium dodecyl sulfate each day from the 3 rd day of coating of sodium dodecyl sulfate in th