CN-121987616-A - Application of morin

Abstract

The invention discloses an application of morin, and relates to the technical field of bacterial quorum sensing inhibition. The application of the morin in preparing the medicine for reducing the enterotoxigenic escherichia coli quorum sensing is provided. The use of 5.0 mg/mL Morin can reduce the quorum sensing of enterotoxigenic E.coli and thus reduce its virulence factor secretion. In the escherichia coli treated by the Morin, the Morin has no obvious inhibition effect on the escherichia coli growth, and the autoinducer-2 (AI-2) and quorum sensing related gene abundance of the Morin are obviously reduced, so that the toxicity and the adhesion capacity of the Morin are reduced. The method for reducing the enterotoxigenic escherichia coli quorum sensing is beneficial to replacing the traditional antibiotics for in-vivo treatment and helps to resist bacterial infection.

Inventors

• JIN CHENGLONG
• WANG XIUQI
• LI HAOYAN
• He Weinuo
• ZHOU JIAYI
• YAN HUICHAO

Assignees

• 华南农业大学

Dates

Publication Date

20260508

Application Date

20260227

Claims (5)

1. 1. The application of the morin in reducing the quorum sensing of enterotoxigenic escherichia coli.
2. 2. The use according to claim 1, wherein said sanguinarine is used to reduce the concentration of autoinducer-2 in enterotoxigenic E.coli, Or the gene abundance related to the quorum sensing of the enterotoxigenic escherichia coli is reduced, Or reduce the toxicity of enterotoxigenic escherichia coli, Or reduce the inhibiting and adhesion capability of enterotoxigenic escherichia coli to IPEC-J2 activity.
3. 3. A composition for reducing the quorum sensing of enterotoxigenic escherichia coli, said composition comprising a sanguinarine, and a pharmaceutically acceptable carrier.
4. 4. A composition according to claim 3, wherein the concentration of morin in the composition is 1.0-10.0 mg/mL.
5. 5. The composition of claim 4, wherein the concentration of morin in the composition is 5.0 mg/mL.

Description

Application of morin Technical Field The invention relates to the technical field of bacterial quorum sensing inhibition, in particular to application of morin in preparing a medicament for reducing quorum sensing of enterotoxigenic escherichia coli. Background Coli can be classified into enterotoxigenic escherichia coli (ETEC), enterohemorrhagic escherichia coli (EHEC), enteropathogenic escherichia coli (EPEC), enteroadhesive escherichia coli (eae), enteroinvasive escherichia coli (EIEC), and Diffuse Adhesive Escherichia Coli (DAEC) according to pathogenic effects. Wherein ETEC is easier to infect young animals, causes diarrhea and even death of the young animals, and is a common zoonotic pathogen with high mortality rate. Quorum Sensing (QS) is an intercellular communication mechanism based on chemical signaling molecules in a microbial population, and is characterized in that microorganisms regulate Quorum-based gene expression and functional behavior according to population density by synthesizing, releasing and sensing signaling molecules such as Quorum sensing molecules (Quorum sensing molecules, QSM). Morusin (Morin, 3,5,7,2',4' -pentahydroxy flavone) is a natural active substance separated from plants, belongs to flavonoid compounds, is a secondary metabolite of phenol in plants, is widely distributed in nature, and has good antioxidant and antiinflammatory effects. At present, research on pathogenic mechanisms of enterotoxigenic escherichia coli has been advanced to a certain extent, but the inhibiting function of the morin on the enterotoxigenic escherichia coli has not been reported yet. Disclosure of Invention In view of this, the present invention provides the use of Phellinus in the manufacture of a medicament for reducing the quorum sensing of enterotoxigenic E.coli. In order to achieve the above purpose, the present invention adopts the following technical scheme: the application of the morin in preparing the medicine for reducing the enterotoxigenic escherichia coli quorum sensing is provided. Further, the morin is used for reducing the concentration of autoinducer-2 in enterotoxigenic escherichia coli, Or the gene abundance related to the quorum sensing of the enterotoxigenic escherichia coli is reduced, Or reduce the toxicity of enterotoxigenic escherichia coli, Or reduce the inhibiting and adhesion capability of enterotoxigenic escherichia coli to IPEC-J2 activity. A composition for reducing the quorum sensing of enterotoxigenic escherichia coli, said composition comprising a sanguinarine, and a pharmaceutically acceptable carrier. Further, the concentration of the morin in the composition is 1.0-10.0 mg/mL. Further, the concentration of morin in the composition is 5.0 mg/mL. Compared with the prior art, the invention has the beneficial effects that: The morin has no obvious inhibition effect on the growth of enterotoxigenic escherichia coli, but has obvious inhibition effect on quorum sensing of bacteria, reduces the secretion of virulence factors and adhesion factors by reducing quorum sensing, and has good application potential in actual defense. The method for reducing the enterotoxigenic escherichia coli quorum sensing is beneficial to preventing and controlling bacterial infection and meets the requirements of environmental protection. The use of 5.0 mg/mL Morin can reduce the quorum sensing of enterotoxigenic E.coli and thus reduce its virulence factor secretion. In the escherichia coli treated by the Morin, the Morin has no obvious inhibition effect on the escherichia coli growth, and the autoinducer-2 (AI-2) and quorum sensing related gene abundance of the Morin are obviously reduced, so that the toxicity and the adhesion capacity of the Morin are reduced. The method for reducing the enterotoxigenic escherichia coli quorum sensing is beneficial to replacing the traditional antibiotics for in-vivo treatment and helps to resist bacterial infection. Drawings In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art. FIG. 1 is a graph showing the bacteriostatic effect of Phellinus linteus on enterotoxigenic E.coli; FIG. 2 is a graph showing the effect of Phellinus on the concentration of E.coli autoinducer-2, which produces enterotoxin, on the abundance of genes related to quorum sensing; FIG. 3 is a graph showing that Phellinus reduces ETEC virulence and reduces ETEC activity against IPEC-J2; FIG. 4 is a graph of the adhesion capacity of Phellinus to reduce the activity of ETEC on IPEC-J2. Detailed Description The following description of the embodimen