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CN-121987663-A - Prussian blue nano-enzyme modified by glycerol

CN121987663ACN 121987663 ACN121987663 ACN 121987663ACN-121987663-A

Abstract

The invention relates to a glycerol-modified Prussian blue nano-enzyme. Specifically, the invention provides a glycerol-modified Prussian blue nano-enzyme. The glycerol-modified Prussian blue nano-enzyme provided by the invention has the advantages of regular morphology, uniform particle size dispersion, excellent dispersibility, high crystallinity and excellent nano-enzyme catalytic activity.

Inventors

  • QIN ZHIGUO
  • YIN SHAOPING

Assignees

  • 江苏省人民医院（南京医科大学第一附属医院）
  • 南京中医药大学

Dates

Publication Date
20260508
Application Date
20260119

Claims (10)

  1. 1. Glycerol-modified Prussian blue nanoenzyme.
  2. 2. The glycerol-modified prussian blue nanoenzyme of claim 1, wherein said glycerol is a coating agent.
  3. 3. A method for preparing a glycerol-modified prussian blue nanoenzyme according to the first aspect of the present invention, the method comprising: Weighing potassium ferricyanide, dissolving in water, adding glycerol, stirring for dissolving, adding hydrochloric acid to obtain a reaction solution, heating the reaction solution for reaction, cooling, and filtering to obtain the Prussian blue nano-enzyme modified by glycerol.
  4. 4. A method according to claim 3, wherein the mass to volume ratio (g; ml) of potassium ferricyanide to glycerol is 1 (70-90), preferably 1 (78-85), more preferably 1 (80-84), most preferably 1:82.
  5. 5. A method according to claim 3, wherein hydrochloric acid is added to a final concentration of 0.005-0.02M, preferably 0.005-0.015M, more preferably 0.008-0.012M, most preferably 0.01M.
  6. 6. A method as claimed in claim 3, wherein the method comprises: Weighing 0.5-0.6g of potassium ferricyanide to be dissolved in 4.5-5.5mL of water, adding 40-50mL of glycerol, stirring and dissolving, adding hydrochloric acid to make the final concentration of the solution be 0.005-0.015M to obtain a reaction solution, placing the reaction solution at 75-85 ℃ to react for 4.5-5.5 h, cooling and filtering to obtain the Prussian blue nano-enzyme modified by the glycerol.
  7. 7. A composition comprising, as a main ingredient, the composition comprises the glycerol-modified Prussian blue nano-enzyme according to claim 1.
  8. 8. Use of the glycerol-modified prussian blue nanoenzyme according to claim 1 for the preparation of a composition for antioxidation.
  9. 9. A method for catalyzing H 2 O 2 , comprising contacting H 2 O 2 with the glycerol-modified Prussian blue nanoenzyme of claim 1, thereby catalyzing H 2 O 2 .
  10. 10. A method for reducing or scavenging active oxygen in a cell, comprising contacting the cell with the glycerol-modified Prussian blue nanoenzyme of claim 1, thereby reducing or scavenging active oxygen in the cell.

Description

Prussian blue nano-enzyme modified by glycerol Technical Field The invention relates to biomedical nano materials, in particular to glycerol modified Prussian blue nano enzyme. Background Prussian blue (Prussian blue, PB) is a typical metal-organic framework coordination polymer, which is widely studied for its excellent electrochemical properties, magnetic properties and gas adsorption capacity. In recent years, with the development of nanobiotechnology, researchers have found that Prussian blue particles (i.e., prussian blue nanoenzymes, PBzymes) having a size on the nanometer scale have catalytic activity that mimics natural enzymes. Specifically, prussian blue nano-enzyme has proved to have activities of peroxidase-like (POD), catalase-like (CAT) and superoxide dismutase-like (SOD), and the multienzyme simulation activity mainly originates from a mixed valence iron ion pair rich in PB crystal lattice, so that the Prussian blue nano-enzyme can be used as an efficient electron donor or acceptor to participate in redox reaction in physiological environment, for example, prussian blue nano-enzyme can catalyze hydrogen peroxide to decompose to generate water or oxygen and can also remove superoxide anions and hydroxyl radicals. Despite the great potential for Prussian blue nanoenzymes, obtaining high quality nanoparticles still faces serious challenges. In order to control the particle size, polyvinylpyrrolidone (PVP) or polyethylene glycol (PEG) is often introduced as a blocking agent or stabilizer, however, the high polymer layer may shield active iron sites on the surface of the nano enzyme, and the contact probability between the high polymer layer and a substrate (such as active oxygen) is remarkably reduced, so that the catalytic activity is inhibited. In addition, the removal of the residual organic reagent often requires a cumbersome washing step, and therefore, how to develop a high-quality Prussian blue nanoenzyme becomes a research hotspot. Therefore, there is a need in the art to develop a high quality Prussian blue nanoenzyme. Disclosure of Invention The invention aims to provide a glycerol-modified Prussian blue nanoenzyme which is regular in morphology, uniform in particle size dispersion, excellent in dispersibility, high in crystallinity and excellent in nanoenzyme catalytic activity. In a first aspect of the invention, a glycerol-modified Prussian blue nanoenzyme is provided. Preferably, the glycerol is a coating agent. Preferably, the particle size of the glycerol-modified Prussian blue nano-enzyme is 30-60nm, preferably 40-55nm, more preferably 45-50nm. In a second aspect of the present invention, there is provided a method for preparing the glycerol-modified prussian blue nanoenzyme according to the first aspect of the present invention, the method comprising: Weighing potassium ferricyanide, dissolving in water, adding glycerol, stirring for dissolving, adding hydrochloric acid to obtain a reaction solution, heating the reaction solution for reaction, cooling, and filtering to obtain the Prussian blue nano-enzyme modified by glycerol. Preferably, the mass to volume ratio (g; ml) of the potassium ferricyanide to the water is (0.07-0.15): 1, preferably (0.09-0.13): 1, more preferably (0.10-0.12): 1, most preferably.0.11:1. Preferably, the mass to volume ratio (g; ml) of potassium ferricyanide to glycerol is 1 (70-90), preferably 1 (78-85), more preferably 1 (80-84), most preferably 1:82. Preferably, hydrochloric acid is added to a final concentration of 0.005-0.02M, preferably 0.005-0.015M, more preferably 0.008-0.012M, most preferably 0.01M. Preferably, the temperature of the heating reaction is from 70 to 90 ℃, preferably from 75 to 85 ℃, more preferably from 78 to 82 ℃, most preferably 80 ℃. Preferably, the heating reaction is for a period of time of 4-6 h, preferably 4.5-5.5 h, more preferably 4.8-5.2 h, most preferably 5h. Preferably, it is cooled to 20-30 ℃, preferably 23-27 ℃, more preferably 25 ℃, and then filtered. Preferably, the filtration comprises filtration through an ultrafiltration tube and a filter membrane in sequence. Preferably, the ultrafiltration tube is centrifugally filtered under conditions of 950-1050G (preferably 980-1020G, more preferably 990-1010G, most preferably 1000G). Preferably, the ultrafiltration tube filters to remove excess glycerol, [ Fe (CN) 6]3-、K+、CN-、H+, and Cl -. Preferably, the membrane pore size of the filter membrane is 0.05-0.2. Mu.m, preferably 0.05-0.15. Mu.m, more preferably 0.08-0.12. Mu.m, most preferably 0.1. Mu.m. Preferably, the filter membrane filters to remove agglomerates. Preferably, the method comprises: Weighing 0.5-0.6g of potassium ferricyanide to be dissolved in 4.5-5.5mL of water, adding 40-50mL of glycerol, stirring and dissolving, adding hydrochloric acid to make the final concentration of the solution be 0.005-0.015M to obtain a reaction solution, placing the reaction solution at 75-85 ℃ to react for 4.5-5.5 h, cooling a