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CN-121987668-A - Application of cell-free fat extract in preparation of medicine for accelerating chronic wound healing

CN121987668ACN 121987668 ACN121987668 ACN 121987668ACN-121987668-A

Abstract

The application relates to the field of biological medicine, and discloses application of a cell-free fat extract in preparation of a medicament for accelerating chronic wound healing. The study of the application shows that CEFFE can improve the healing of chronic wounds of diabetic mice. This is achieved by the pro-angiogenic and anti-inflammatory activity of CEFFE. The presence of various growth factors in CE FFE may explain its effect on the functions of epithelial cells, endothelial cells, fibroblasts and macrophages. CEFFEE can be used as a therapeutic drug for treating chronic wounds of diabetics, and is expected to improve the overall prognosis of the diabetics and the life quality of the diabetics.

Inventors

  • HAN JUNFENG

Assignees

  • 上海市同济医院

Dates

Publication Date
20260508
Application Date
20241108

Claims (7)

  1. 1. Use of cell-free fat extract in the preparation of a medicament for accelerating the healing of chronic wounds.
  2. 2. The use according to claim 1, wherein the cell-free fat extract accelerates skin wound healing, re-epithelialization and collagen deposition.
  3. 3. The use of claim 1, wherein the chronic wound healing comprises a chronic refractory wound of a diabetic patient.
  4. 4. A medicament for accelerating the healing of chronic wounds, comprising a cell-free fat extract as an active substance.
  5. 5. The medicament of claim 4, further comprising other active substances that accelerate healing of chronic wounds.
  6. 6. The medicament of claim 5, further comprising a pharmaceutically acceptable carrier or adjuvant.
  7. 7. The medicament according to claim 4, wherein the pharmaceutical dosage form comprises a tablet, a powder, a granule, a capsule, an oral liquid, an injection or a sustained release agent.

Description

Application of cell-free fat extract in preparation of medicine for accelerating chronic wound healing Technical Field The application relates to the field of biological medicine, in particular to application of a cell-free fat extract in preparation of a medicament for accelerating chronic wound healing. Background Chronic refractory wounds in diabetics remain a clinical problem to be solved. Chronic wounds can lead to secondary injuries such as infections, chronic foot ulcers and limb injuries, severely affecting the quality of life of the patient. In order to promote wound healing, many methods have been tried, such as debridement, antibiotic treatment, tissue engineering materials, cell treatment, and the like. Among them, stem cell-based therapies are considered as a promising approach to the treatment of chronic wounds. Adipose Stem Cells (ASCs) are one of the Mesenchymal Stem Cells (MSCs) and show various advantages in wound healing. ASCs can differentiate into a variety of cells involved in wound healing, such as fibroblasts, keratinocytes, epithelial cells and endothelial cells, and promote differentiation and proliferation of stem cells and their neighboring cells, accelerating the wound healing process by paracrine secretion of various growth factors including basic fibroblast growth factor (bFGF), insulin-like growth factor-1 (IGF-1), hepatocyte Growth Factor (HGF), vascular Endothelial Growth Factor (VEGF) and transforming growth factor- β1 (TGF- β1). In addition, ASCs have anti-apoptotic and antioxidant properties, which can further improve the wound healing process. Although ASCs are very effective in improving wound healing, there are limitations in their use, mainly in the specificity of donor sources and in the quality of cell culture. Adipose tissue is a rich source of ASCs, secreting large amounts of bFGF, VEGF, and Platelet Derived Growth Factor (PDGF), promoting angiogenesis and adipogenesis. This suggests that adipose tissue is rich in a variety of bioactive factors that can be isolated directly for clinical use without cell isolation or culture. The cell-free fat extract (CEFFE) is a liquid fraction extracted from adipose tissue, and analysis shows that it contains a large amount of cytokines and growth factors including IGF-1, TGF- β1, HGF, VEGF, PDGF, bFGF, brain-derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), and the like. The mechanism of action of CEFFE in accelerating chronic wound healing has not been reported after review of the publications. Disclosure of Invention The invention aims at researching the effect of CEFFE on accelerating the healing of chronic wounds and discloses the application of cell-free fat extract in preparing medicines for accelerating the healing of chronic wounds. In order to achieve the above object, the present application provides the following technical solutions: In a first aspect, the application provides the use of a cell-free fat extract for the preparation of a medicament for accelerating the healing of a chronic wound. Further, the cell-free fat extract accelerates skin wound healing, re-epithelialization and collagen deposition. Further, the chronic wound healing includes chronic refractory wounds of diabetics. In a second aspect, the present application provides a medicament for accelerating the healing of chronic wounds, comprising a cell-free fat extract as an active substance. Further, other active substances that can accelerate the healing of chronic wounds are included. Further, the composition also comprises pharmaceutically acceptable carriers or auxiliary materials. Further, the pharmaceutical dosage forms comprise tablets, powder, granules, capsules, oral liquid, injection or sustained release agents. In summary, the application has the following beneficial effects: The study of the invention shows that CEFFE can improve the healing of chronic wounds of diabetic mice. This is achieved by the pro-angiogenic and anti-inflammatory activity of CEFFE. CEFFE the presence of various growth factors may explain their effects on the functions of epithelial cells, endothelial cells, fibroblasts and macrophages. CEFFE can be used as a therapeutic drug for treating chronic wounds of diabetics, and is expected to improve the overall prognosis of the diabetics and the life quality of the diabetics. Drawings FIG. 1 is a schematic of CEFFE promoting wound healing in db/db mice; FIG. 2 CEFFE topical application accelerates wound healing in db/db mice; (A: representative images of wound healing in PBS, CEFFE high dose and CEFFE low dose groups; B: percentage of original wound area in each of the groups on day 0, day 3, day 7, day 10 and day 14 of the experiment; C: time required for wound healing in db/db mice; data expressed as mean ± standard deviation; n=6, P <0.05, P < 0.01); FIG. 3 CEFFE topical application accelerates skin wound healing, re-epithelialization and collagen deposition in db/db mice; (A: H & E staining