CN-121987672-A - Application of bifidobacterium breve NX-5 in preparation of medicine for relieving anxiety and improving sleep

Abstract

The invention discloses an application of bifidobacterium breve NX-5 in preparing a medicament for relieving anxiety and improving sleep, and belongs to the technical field of microorganisms. The invention discloses application of bifidobacterium breve NX-5 in preparing a medicament for relieving anxiety and improving sleep, wherein bacterial suspension of bifidobacterium breve NX-5 can obviously reduce the movement distance, mania time and active time of zebra fish and obviously increase resting time in an in-vivo insomnia model, and has the potential of being applied to relieving anxiety and improving sleep in vivo. The bifidobacterium breve NX-5 disclosed by the invention has great potential application prospect in the aspects of relieving anxiety and improving sleep.

Inventors

• ZHENG KANGDI
• MA YAN
• CHEN DAN
• ZHANG ZHAO

Assignees

• 广东南芯医疗科技有限公司
• 广东一元兰欣生物科技有限公司

Dates

Publication Date

20260508

Application Date

20230228

Claims (4)

1. 1. The application of the bifidobacterium breve NX-5 in preparing the medicine for relieving anxiety and improving sleep is characterized in that the preservation number of the bifidobacterium breve NX-5 is CGMCC No.20113.
2. 2. The use of bifidobacterium breve NX-5 according to claim 1 for the preparation of a medicament for alleviating anxiety and improving sleep, wherein said bifidobacterium breve NX-5 is a bacterial suspension.
3. 3. Use of bifidobacterium breve NX-5 as claimed in claim 1 in the preparation of fermented dairy products and other food products.
4. 4. Use of bifidobacterium breve NX-5 according to claim 3 for the preparation of fermented dairy products and other food products, characterized in that said bifidobacterium breve NX-5 is a bacterial suspension.

Description

Application of bifidobacterium breve NX-5 in preparation of medicine for relieving anxiety and improving sleep Technical Field The invention relates to the technical field of microorganisms, in particular to application of bifidobacterium breve NX-5 in preparation of a medicament for relieving anxiety and improving sleep. Background Sleep is closely related to our life, however, with the rapid development of society, people are increasingly stressed, and insomnia is becoming a common phenomenon. Insomnia may be accompanied by anxiety, depression, etc. At present, the most common clinical medicine for relieving anxiety and treating sleep disorder is benzodiazepines. However, these drugs may have various degrees of side effects such as dizziness, fatigue, sleepiness, inattention, fear, dysphoria, hallucinations, even temporary forgetfulness and hangover, and excessively prolonged sleep time. They do not truly achieve the desired effect of alleviating anxiety and improving sleep quality. Therefore, the selection of natural, side-effect-free ingredients is an important approach to alleviating anxiety and treating sleep disorders. Gamma-aminobutyric acid (GABA) is an important neurotransmitter. In recent years, research and development of GABA-enriched foods have become a hotspot of research at home and abroad. The research shows that GABA can better relieve mental stress, regulate emotion and has certain relieving effects on anxiety and insomnia. Also studies have shown that sleep disorders lead to metabolic disorders with minor changes in intestinal microbiota. Recently, some studies have reported preliminary evidence that the intestinal microbiota affects sleep. Mice that take antibiotics for long periods of time exhibit sleep disturbances accompanied by changes in intestinal neurotransmitters. Furthermore, it was found that dietary probiotics can effectively improve stress-affected sleep conditions. These results indicate that remodelling the microbiota may improve the sleep quality of the host through MGBA. However, probiotics are currently less studied and used in alleviating anxiety and improving sleep. The probiotics strain used by domestic production enterprises depends on import for a long time, and foreign strains are not necessarily suitable for gastrointestinal tract physiological conditions of residents in China. In addition, the lack of powerful scientific research evidence of the function of probiotics seriously affects the popularization of the probiotics and products thereof. Based on the method, aiming at the function deep excavation of strain resources, the novel probiotic bacterial strain which has independent intellectual property, specific functional property and physiological property suitable for Chinese crowd is screened out, and the method is particularly important for improving the core competitiveness of Chinese probiotic production enterprises and promoting the development of Chinese probiotic products. Therefore, providing the application of bifidobacterium breve NX-5 in preparing medicines for relieving anxiety and improving sleep is a problem to be solved urgently by those skilled in the art. Disclosure of Invention In view of the above, the invention provides application of bifidobacterium breve NX-5 in preparing medicines for relieving anxiety and improving sleep. Zebra fish share a similar nervous system as mammals. Molecules and cell populations that play a role in sleep regulation in mammals are also present for the most part in zebra fish. The response of various hypnotics such as melatonin agonists and gamma-aminobutyric acid (GABA) modulators in zebra fish is also similar to mammals. GABA is a naturally occurring non-protein amino acid and is an important inhibitory neurotransmission substance in the central nervous system of mammals. Pentatetrazole (PTZ) is a GABA inhibitor and small doses of PTZ can induce bipolar disorder with both mania and depression, resulting in insomnia. The zebra fish insomnia model can show abnormal behaviors, including the total movement distance, manic time and active time which are obviously increased, and the abnormal behaviors can be observed through a behavior analyzer. In order to achieve the above purpose, the present invention adopts the following technical scheme: The application of bifidobacterium breve NX-5 in preparing a medicament for relieving anxiety and improving sleep, wherein the preservation number of the bifidobacterium breve (Bifidobacteriumbreve) NX-5 is CGMCC No.20113 (see patent number 202110372822.2). In the PTZ induced zebra fish insomnia model, the bifidobacterium breve NX-5 can remarkably reduce the movement distance, mania time and active time of the zebra fish, remarkably increase the resting time, and has good probiotic effects of relieving anxiety and improving sleep. Further, the bifidobacterium breve NX-5 is applied to the preparation of fermented dairy products and other foods. Further, the bifidobacterium bre