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CN-121987681-A - Malignant tumor postoperative irrigation agent and preparation method and application thereof

CN121987681ACN 121987681 ACN121987681 ACN 121987681ACN-121987681-A

Abstract

The invention discloses a malignant tumor postoperative irrigation agent, a preparation method and application thereof. Extracting lignum sappan decoction pieces under reflux by heating after adding water to obtain extractive solution, filtering the extractive solution, concentrating the filtrate to obtain concentrated solution, adding ethanol into the concentrated solution, standing for precipitation, vacuum filtering or centrifuging to obtain first supernatant, recovering ethanol from the first supernatant under reduced pressure to obtain lignum sappan water extractive solution, adding water into the lignum sappan water extractive solution, adjusting the solution ratio to a preset value, adding antioxidant, stirring for dissolving, standing, filtering to obtain second supernatant, adding water into the second supernatant, adjusting the solution ratio to a preset value, adding excipient, and stirring for dissolving. The malignant tumor postoperative irrigation solution with the sappan wood extract as an active ingredient is used for local irrigation of an operation wound surface, has the effect of killing various cancer cells, and can effectively remove residual tumor cells, so that postoperative recurrence and metastasis of tumors are prevented.

Inventors

  • YANG XIHUA
  • MA JINFENG
  • ZHAO LILI
  • CHEN LIXIA
  • LI FENG

Assignees

  • 山西省肿瘤医院

Dates

Publication Date
20260508
Application Date
20260210

Claims (10)

  1. 1. A postoperative irrigation solution for malignant tumor is characterized by comprising sappan wood water extraction liquid medicine.
  2. 2. The postoperative malignant tumor irrigating agent according to claim 1, further comprising an antioxidant and an excipient, wherein the antioxidant and the excipient are 0.05-0.20% of the mass of the sappan wood water extract liquid medicine.
  3. 3. The postoperative malignant tumor irrigating agent according to claim 2, wherein said antioxidant is selected from the group consisting of vitamin C, sodium bisulphite and EDTA-2Na.
  4. 4. The postoperative malignant rinsing agent according to claim 2 or 3, wherein said excipient is selected from mannitol, sucrose or dextran 70.
  5. 5. A method of preparing the post-operative malignant tumor irrigant according to any one of claims 1 to 4, comprising: step one, taking sappan wood decoction pieces, adding water, heating and reflux-extracting to obtain an extracting solution, filtering the extracting solution, and concentrating the filtrate to obtain a concentrated solution; Adding ethanol into the concentrated solution, standing for precipitation, performing suction filtration or centrifugation to obtain a first supernatant, recovering the ethanol in the first supernatant under reduced pressure to obtain a sappan wood water extraction liquid medicine, adding water into the sappan wood water extraction liquid medicine, adjusting the liquid medicine ratio to a preset value, adding an antioxidant, stirring for dissolution, standing, and filtering to obtain a second supernatant; and thirdly, adding water into the second supernatant, adjusting the liquid medicine ratio to a preset value, adding excipient, and stirring for dissolution.
  6. 6. The method of claim 5, wherein the water is added in an amount 8-12 times the amount of the hematoxylin pieces in the first step.
  7. 7. The method of claim 5 or 6, wherein in the first step, the heat reflux extraction is heat boiling reflux extraction.
  8. 8. The method for preparing a malignant tumor postoperative irrigation solution according to claim 7, wherein in the first step, the filtrate is transferred to a double-effect concentrator and concentrated under reduced pressure under the conditions of 60-70 ℃ and vacuum degree of-0.06 to-0.09 MPa, and the relative density of the concentrated solution at 60 ℃ is 1.03-1.05.
  9. 9. The method of claim 5, wherein the ratio of the liquid medicine in the second step to the third step is 1:1.
  10. 10. Use of the postoperative irrigation agent for malignant tumor according to claims 1 to 4 for the preparation of a medicament for the prevention or treatment of postoperative recurrence or metastasis of malignant tumor by local irrigation of a surgical wound.

Description

Malignant tumor postoperative irrigation agent and preparation method and application thereof Technical Field The invention belongs to the technical field of medicines, and particularly relates to a malignant tumor postoperative irrigation agent and a preparation method and application thereof. Background Surgery is one of the main means of malignant tumor treatment, but a small amount of tumor tissues and tumor cells are easy to remain after surgery, thereby causing tumor recurrence and metastasis. Therefore, the application of the drug for preventing tumor recurrence after operation after malignant tumor operation can be used for flushing so as to remove residual tumor tissues and tumor cells, and the purpose of preventing tumor recurrence and metastasis can be achieved. Most of postoperative flushing solutions commonly used clinically at present are normal saline or solutions containing antibacterial drugs, and the antitumor effect of the postoperative flushing solutions is limited. The traditional Chinese medicine sappan wood has anti-tumor activity, but the application of the traditional Chinese medicine sappan wood in a postoperative irrigation agent is not yet reported, so that the traditional Chinese medicine sappan wood has very important significance in developing the traditional Chinese medicine sappan wood into an irrigation agent for postoperative irrigation of malignant tumors. Disclosure of Invention The invention aims to solve the technical problem of providing a malignant tumor postoperative irrigation agent, a preparation method and application thereof, wherein the irrigation agent can effectively prevent postoperative recurrence and metastasis of malignant tumor. In order to solve the technical problems, according to one aspect of the invention, a postoperative cleaning agent for malignant tumor is provided, which comprises sappan wood water extract liquid medicine. As a preferred embodiment, the pharmaceutical composition also comprises 0.05-0.20% of antioxidant and 2-5% of excipient, wherein the antioxidant and the excipient are equivalent to the mass of the sappan wood water extract liquid medicine. In a preferred embodiment, the antioxidant is selected from vitamin C, sodium bisulphite or EDTA-2Na. In a preferred embodiment, the excipient is selected from mannitol, sucrose or dextran 70. According to another aspect of the present invention, there is provided a method for preparing the above-described malignant tumor postoperative irrigation agent, comprising: step one, taking sappan wood decoction pieces, adding water, heating and reflux-extracting to obtain an extracting solution, filtering the extracting solution, and concentrating the filtrate to obtain a concentrated solution; Adding ethanol into the concentrated solution, standing for precipitation, performing suction filtration or centrifugation to obtain a first supernatant, recovering the ethanol in the first supernatant under reduced pressure to obtain a sappan wood water extraction liquid medicine, adding water into the sappan wood water extraction liquid medicine, adjusting the liquid medicine ratio to a preset value, adding an antioxidant, stirring for dissolution, standing, and filtering to obtain a second supernatant; and thirdly, adding water into the second supernatant, adjusting the liquid medicine ratio to a preset value, adding excipient, and stirring for dissolution. In the first step, the water is added in an amount 8-12 times that of the sappan wood decoction pieces. In a preferred embodiment, in the first step, the heat reflux extraction is heat boiling reflux extraction. In the first step, the filtrate is transferred to a double-effect concentrator and concentrated under reduced pressure under the conditions of 60-70 ℃ and vacuum degree of-0.06 to-0.08 MPa, and the relative density of the concentrated solution at 60 ℃ is 1.03-1.05. In the second and third steps, the preset liquor ratio is 1:1. Another exemplary embodiment of the present invention provides the use of the above-described post-malignant tumor irrigating agent for preparing a medicament for preventing or treating postoperative recurrence or metastasis of malignant tumor by locally irrigating a surgical wound. The invention provides a malignant tumor postoperative irrigation solution taking sappan wood extract as an active ingredient, which is used for local irrigation of an operation wound surface, shows the killing effect on various cancer cells, and can effectively remove residual tumor cells, thereby preventing postoperative recurrence and metastasis of tumors, and has definite clinical conversion value and market application prospect. Detailed Description The postoperative cleaning agent for malignant tumors provided by the typical embodiment of the invention comprises sappan wood water extraction liquid medicine. The malignant tumor postoperative irrigation solution provided by the embodiment is prepared from sappan wood decoction pieces servi