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CN-121987697-A - Preparation method of natural antioxidant-rich pet anti-aging life-prolonging cell nutrient

CN121987697ACN 121987697 ACN121987697 ACN 121987697ACN-121987697-A

Abstract

The invention discloses a preparation method of a natural antioxidant-rich pet anti-aging life-prolonging cell nutrient, belonging to the field of pet nutrition supplements. Firstly, mixing blueberry powder, broccoli powder, carrot powder, spinach powder, pomegranate extract, green tea extract, curcumin, fish oil, vitamin E, vitamin C and water according to a specific proportion, then, carrying out composite enzymolysis treatment, using the synergistic effect of cellulase and pectase to fully release active substances in plant cells, then, fermenting enzymolysis liquid by lactic acid bacteria, converting nutrition components and generating flavor substances, then, adding lecithin to carry out high-speed shearing and homogenizing emulsification to form stable emulsion so as to protect fat-soluble components and improve physical properties, and finally, preparing a final product by an optimized spray drying process. The method solves the problems of insufficient release of active ingredients, poor stability, low bioavailability, poor palatability and the like in the prior art through scientific compatibility of raw materials and targeted process optimization.

Inventors

  • LIU JIAMEI
  • ZHANG CHUANHONG
  • ZHANG SHIXIN

Assignees

  • 北京中农劲腾生物制药股份有限公司

Dates

Publication Date
20260508
Application Date
20260205

Claims (10)

  1. 1. A preparation method of a natural antioxidant-rich pet anti-aging life-prolonging cell nutrient is characterized by comprising the following steps: (1) Raw material pretreatment, namely uniformly mixing, by weight, 10-20 parts of blueberry powder, 5-15 parts of broccoli powder, 5-10 parts of carrot powder, 3-8 parts of spinach powder, 2-5 parts of pomegranate extract, 1-3 parts of green tea extract, 0.5-2 parts of curcumin, 2-5 parts of fish oil, 0.1-0.5 part of vitamin E, 0.1-0.5 part of vitamin C and 50-100 parts of water; (2) Adding complex enzyme into the mixture in the step (1), controlling the final addition amount of cellulase to be 1000-5000U/g of the mixture and the final addition amount of pectase to be 2000-8000U/g of the mixture, performing enzymolysis for 1-2 hours under the condition of 40-50℃, pH 4.5-5.5, and then heating to 90 ℃ for sterilization for 10 minutes; (3) Microbial fermentation, namely cooling the enzymolysis liquid obtained in the step (2) to 30-35 ℃, inoculating 1-3% of lactobacillus suspension, and fermenting for 24-48 hours at 30-37 ℃; (4) Homogenizing and emulsifying, namely adding lecithin accounting for 1% -3% of the total weight of the fermentation broth in the step (3), and performing high-speed shearing and homogenizing and emulsifying at the temperature of 60-70 ℃ and the rotating speed of 10000-15000rpm for 2-4 minutes; (5) And (3) drying, namely spray-drying the emulsion obtained in the step (4), wherein the air inlet temperature of spray-drying is 160-180 ℃, and the air outlet temperature is 70-80 ℃, so as to obtain the powdery cell nutrient.
  2. 2. The preparation method of claim 1, wherein in the step (1), the weight parts ratio of the blueberry powder, the broccoli powder and the carrot powder is (12-18): 8-12): 6-8.
  3. 3. The method according to claim 1, wherein in the step (2), the cellulase and pectase are added in a ratio of 1 (2-4).
  4. 4. The method according to claim 1, wherein in the step (3), the lactic acid bacteria is Lactobacillus acidophilus, and the concentration of the bacterial suspension is 1X 10-8-1X 10-9 CFU/mL.
  5. 5. The method according to claim 1, wherein in the step (4), the lecithin is soybean lecithin having a phospholipid content of not less than 60%.
  6. 6. The method of claim 1, wherein in step (5), the spray drying is performed using a pressure atomizer having an atomization pressure of 0.2 to 0.4 MPa.
  7. 7. The method according to claim 1, wherein in the step (1), the procyanidine content of the pomegranate extract is not less than 40%, and the catechin content of the green tea extract is not less than 50%.
  8. 8. A pet anti-aging life-prolonging cell nutrient prepared by the preparation method of any one of claims 1-7, wherein the total antioxidant capacity of the nutrient is improved by more than 80% compared with the physical mixing of single raw materials in terms of ORAC value, and the bioavailability of the nutrient is improved by more than 50%.
  9. 9. The pet anti-aging life-prolonging cell nutrient of claim 8, wherein the release rate in the simulated environment of the gastrointestinal tract of the pet is greater than 90%, the particle size is less than 50 μm, and the water dispersibility time is less than 30 seconds.
  10. 10. Use of the pet anti-aging life-prolonging cell nutrient of claim 8 in the preparation of a pet anti-aging pharmaceutical formulation.

Description

Preparation method of natural antioxidant-rich pet anti-aging life-prolonging cell nutrient Technical Field The invention belongs to the field of pet nutrition supplements, and particularly relates to a preparation method of a natural antioxidant-enriched pet anti-aging life-prolonging cell nutrient. Background With the improvement of the home status of the pets and the improvement of the pet keeping concept, the pet owners pay more attention to the health aging and life quality prolongation of the pets. Through the nutrition intervention means, the normal physiological functions of the pet organism in the aging stage are supported, and the cell health is maintained, so that the method has become an important research direction in the field of pet nutrition. The natural antioxidant has natural sources, various biological activities and high safety, and has wide prospect in the development of anti-aging and life-prolonging nutrition for pets. However, the existing commercial pet nutritional supplements still have significant shortcomings in terms of antioxidant and anti-aging life-prolonging functions. First, most products are simply physical blends of functional materials in formulation design, lacking systematic consideration of synergistic interaction between the components. For example, antioxidants such as blueberry powder and green tea extract are directly mixed, so that differences of different antioxidants in absorption, distribution, metabolism and action targets are ignored, and the overall antioxidation efficiency of the antioxidant cannot realize the cooperative promotion of 1+1>2, so that the bioavailability is limited. Secondly, in the preparation process, the traditional technology has insufficient damage to the cell wall structure of the raw material, so that natural antioxidants (such as procyanidins in the pomegranate and catechin in the green tea) embedded in cells are difficult to fully release. The conventional pulverizing and mixing process has low extraction efficiency, and the active ingredients Yi Yinguang, heat, oxygen and other factors are deactivated in the subsequent processing and storage processes, so that the stability and effectiveness of the final product are greatly reduced. In addition, the roughness of the process also affects the palatability and physical properties of the product, such as large granularity and poor water dispersibility, and is unfavorable for the ingestion and digestion and absorption of pets, especially the elderly pets. Moreover, the processing process in the prior art may damage heat-sensitive nutrients (such as certain vitamins and active factors) in the raw materials, and meanwhile, macromolecular substances cannot be converted into small-molecular nutrients which are easier to be absorbed by the intestinal tracts of pets through effective technical means (such as enzymolysis and fermentation), so that the development of the nutritional value of the pets is further limited. In the aspects of formulation design and application, optimization aiming at physiological characteristics and eating habits of pets is lacking, so that the product has insufficient convenience and low compliance in actual use. Therefore, the pet nutrition industry is urgent to develop a cell nutrient preparation method capable of systematically integrating various natural antioxidants, maximally retaining and improving the activity of the cell nutrient through an innovative preparation process, and simultaneously having excellent stability and pet feeding compliance, so as to meet the urgent requirements of the market on efficient, safe and convenient pet anti-aging life-prolonging nutrition. Disclosure of Invention In order to solve the problems, the invention provides a preparation method of a natural antioxidant-rich pet anti-aging life-prolonging cell nutrient. The aim of the invention is achieved by the following technical scheme. A preparation method of a natural antioxidant-rich pet anti-aging life-prolonging cell nutrient comprises the following steps: (1) Raw material pretreatment, namely uniformly mixing, by weight, 10-20 parts of blueberry powder, 5-15 parts of broccoli powder, 5-10 parts of carrot powder, 3-8 parts of spinach powder, 2-5 parts of pomegranate extract, 1-3 parts of green tea extract, 0.5-2 parts of curcumin, 2-5 parts of fish oil, 0.1-0.5 part of vitamin E, 0.1-0.5 part of vitamin C and 50-100 parts of water; (2) Adding complex enzyme into the mixture in the step (1), controlling the final addition amount of cellulase to be 1000-5000U/g of the mixture and the final addition amount of pectase to be 2000-8000U/g of the mixture, performing enzymolysis for 1-2 hours under the condition of 40-50℃, pH 4.5-5.5, and then heating to 90 ℃ for sterilization for 10 minutes; (3) Microbial fermentation, namely cooling the enzymolysis liquid obtained in the step (2) to 30-35 ℃, inoculating 1-3% of lactobacillus suspension, and fermenting for 24-48 hours at 30-37 ℃;