CN-121987778-A - Application of nucleic acid molecule for encoding anti-novel goose astrovirus nano antibody or encoded anti-novel goose astrovirus nano antibody in preparation of anti-novel goose astrovirus medicament

Abstract

The invention provides a nucleic acid molecule for encoding a novel goose astrovirus-resistant nano antibody or application of the novel goose astrovirus-resistant nano antibody encoded by the nucleic acid molecule in preparing a novel goose astrovirus-resistant medicament, and belongs to the technical field of biological products. A nucleic acid molecule encoding an anti-novel amanita virus (nGAstV) nanobody (Nb) comprising, sequentially linked, a 5 '-untranslated region, a sequence encoding a signal peptide, a coding sequence for Nb, a 3' -untranslated region, and a poly (a) tail. The nucleic acid molecule can efficiently and stably express the Nb with immunological activity in vitro and in vivo, and experiments prove that the Nb has activity of inhibiting nGAstV replication, and the Nb neutralizes nGAstV by blocking the adsorption and invasion of nGAstV to host cells. The invention provides a new way for treating goose gout and a new means for developing medicines for treating fowl goose gout.

Inventors

• ZHU QIYUN
• WANG DAN
• JI YANHONG
• AI YI

Assignees

• 中国农业科学院兰州兽医研究所(中国动物卫生与流行病学中心兰州分中心)

Dates

Publication Date

20260508

Application Date

20260211

Claims (10)

1. 1. The application of the novel goose astrovirus-resistant nano antibody in preparing medicaments for resisting novel goose astroviruses is provided, wherein the amino acid sequence of the novel goose astrovirus-resistant nano antibody is shown as SEQ ID NO. 3.
2. 2. A nucleic acid molecule encoding an anti-novel amanita nanobody comprising the following linked genetic elements: a 5 '-untranslated region, a sequence encoding a signal peptide, a coding sequence for an anti-novel goose astrovirus nanobody, a 3' -untranslated region, and a poly (A) tail; the amino acid sequence of the novel goose astrovirus-resistant nano antibody is shown as SEQ ID NO. 3.
3. 3. The nucleic acid molecule of claim 2, wherein the 5 '-untranslated region and the 3' -untranslated region comprise a 5'-UTR sequence and a 3' -UTR sequence or mutated sequences thereof derived from a goose α -globin gene or a novel goose astrovirus gene; The nucleotide sequence of the 5'-UTR mutant sequence of the goose alpha-globin gene source is shown as SEQ ID NO. 6, and the nucleotide sequence of the 3' -UTR mutant sequence of the goose alpha-globin gene source is shown as SEQ ID NO. 7; the nucleotide sequence of the novel goose astrovirus gene-derived 5' -UTR mutant sequence is shown as SEQ ID NO. 8; the nucleotide sequence of the novel goose astrovirus gene-derived 3' -UTR mutant sequence is shown as SEQ ID NO. 9.
4. 4. The nucleic acid molecule of claim 2, wherein the signal peptide comprises a signal peptide of a goose-derived cytokine.
5. 5. The nucleic acid molecule according to any one of claims 2 to 4, wherein the DNA sequence of the nucleic acid molecule comprises a nucleotide sequence as shown in SEQ ID NO. 1 and/or SEQ ID NO. 2.
6. 6. A nucleic acid nanolipid carrier comprising a lipid nanoparticle carrying a nucleic acid molecule according to any one of claims 2 to 5.
7. 7. The nucleic acid nanolipid carrier of claim 6, wherein the lipid nanoparticle material comprises at least one of an ionizable lipid, a helper phospholipid, cholesterol, and a pegylated lipid.
8. 8. A novel goose astrovirus-resistant drug, characterized in that the active ingredient comprises the nucleic acid molecule according to any one of claims 2-5 or the nucleic acid nanolipid carrier according to claim 6 or 7.
9. 9. Use of the nucleic acid molecule according to any one of claims 2 to 5 or the nucleic acid nanolipid vector according to claim 6 or 7 in the preparation of a medicament against a novel goose astrovirus.
10. 10. The use of claim 9, wherein said combating new goose astrovirus comprises inhibiting replication of new goose astrovirus.

Description

Application of nucleic acid molecule for encoding anti-novel goose astrovirus nano antibody or encoded anti-novel goose astrovirus nano antibody in preparation of anti-novel goose astrovirus medicament Technical Field The application belongs to the technical field of biological products, and in particular relates to a nucleic acid molecule for encoding a novel goose astrovirus-resistant nano antibody or application of the novel goose astrovirus-resistant nano antibody encoded by the nucleic acid molecule in preparing a novel goose astrovirus-resistant medicament. Background The novel goose astrovirus (Novel Goose Astrovirus, nGAstV) is a pathogenic pathogen of goose gout, has high susceptibility to gosling within 3 weeks of age, and can cause serious clinical symptoms and even death. At present, aiming at the gout of gosling caused by nGAstV, no commercial vaccine or specific therapeutic medicine is applied in batches, and an effective prevention and control means is lacked clinically. Disclosure of Invention The invention provides an application of an anti-novel goose astrovirus nano antibody in preparing an anti-novel goose astrovirus drug. The invention provides application of an anti-novel goose astrovirus nano antibody in preparing a medicament for resisting novel goose astrovirus, wherein the amino acid sequence of the anti-novel goose astrovirus nano antibody is shown as SEQ ID NO. 3. The invention provides a nucleic acid molecule for encoding an anti-novel goose astrovirus nanobody, which comprises the following connected gene elements: a 5 '-untranslated region, a sequence encoding a signal peptide, a coding sequence for an anti-novel goose astrovirus nanobody, a 3' -untranslated region, and a poly (A) tail; the amino acid sequence of the novel goose astrovirus-resistant nano antibody is shown as SEQ ID NO. 3. Preferably, the 5'-UTR sequence and the 3' -UTR sequence or the mutant sequence thereof are derived from the goose alpha-globin gene or from the novel goose astrovirus gene; The nucleotide sequence of the 5'-UTR mutant sequence of the goose alpha-globin gene source is shown as SEQ ID NO. 6, and the nucleotide sequence of the 3' -UTR mutant sequence of the goose alpha-globin gene source is shown as SEQ ID NO. 7; the nucleotide sequence of the novel goose astrovirus gene-derived 5' -UTR mutant sequence is shown as SEQ ID NO. 8; the nucleotide sequence of the novel goose astrovirus gene-derived 3' -UTR mutant sequence is shown as SEQ ID NO. 9. Preferably, the signal peptide comprises a signal peptide of a goose-derived cytokine. Preferably, the DNA sequence of the nucleic acid molecule comprises the nucleotide sequence shown as SEQ ID NO. 1 and/or SEQ ID NO. 2. The invention provides a nucleic acid nano lipid carrier, which comprises lipid nano particles loaded with the nucleic acid molecules. Preferably, the lipid nanoparticle material comprises at least one of an ionizable lipid, a helper phospholipid, cholesterol, and a pegylated lipid. The invention provides a novel goose astrovirus-resistant drug, and an active ingredient comprises the nucleic acid molecule or the nucleic acid nano lipid carrier. The invention provides application of the nucleic acid molecule or the nucleic acid nano lipid carrier in preparing medicines for resisting novel goose astrovirus. Preferably, the combating the novel goose astrovirus comprises inhibiting replication of the novel goose astrovirus. The invention provides application of an anti-novel goose astrovirus nano antibody in preparing a medicament for resisting novel goose astrovirus, wherein the amino acid sequence of the anti-novel goose astrovirus nano antibody is shown as SEQ ID NO. 3. The experiment of the invention adopts an indirect ELISA method to detect the expression of the anti-novel goose astrovirus nano-antibody in and out of the body, and at the same time, the anti-novel goose astrovirus nano-antibody is further proved to have the activity of inhibiting nGAstV replication, so that the anti-novel goose astrovirus nano-antibody has the activity of resisting nGAstV. In view of the fact that the novel goose astrovirus is a pathogenic pathogen causing goose gout, the anti-novel goose astrovirus nano antibody provides a novel approach for treating goose gout and a novel means for developing medicaments for treating goose gout. The invention provides a nucleic acid molecule for encoding an anti-novel goose astrovirus nano antibody, which comprises the following connected genetic elements of a5 '-untranslated region, a sequence for encoding a signal peptide, a coding sequence for the anti-novel goose astrovirus nano antibody, a 3' -untranslated region and a poly (A) tail, wherein the amino acid sequence of the anti-novel goose astrovirus nano antibody is shown as SEQ ID NO. 3. According to the invention, the untranslated regions are respectively added at the two ends of the coding sequence of the novel goose astrovirus-resistant nano antibody, so that t