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CN-121987781-A - Composite nano material and preparation and application thereof

CN121987781ACN 121987781 ACN121987781 ACN 121987781ACN-121987781-A

Abstract

The invention provides a composite nano material, and preparation and application thereof. Specifically, the invention provides a composite nano material, which comprises a core material A and a stabilizer B, wherein the core material A comprises Mo-doped WO 3 nano sheets and noble metal nano enzyme loaded on the Mo-doped WO 3 nano sheets. The invention combines the strategies of enzyme catalysis treatment, acoustic power treatment, reactive Oxygen Species (ROS) generation and Glutathione (GSH) consumption, and explores the excellent performance and in-vitro anti-tumor effect of the composite nano material. The composite nano material can be used for preparing a pharmaceutical composition for treating tumors by combining acoustic power and enzyme catalysis, or can be used as a carrier of a tumor drug and the like.

Inventors

  • ZHAO DE
  • SHI MIN
  • WANG YUGANG
  • XU LING
  • WU QIONG
  • WANG NA
  • WANG CHEN

Assignees

  • 上海市同仁医院

Dates

Publication Date
20260508
Application Date
20241108

Claims (10)

  1. 1. A composite nanomaterial comprising a core material a and a stabilizer B; Wherein, the core material A comprises a Mo-doped WO 3 nanometer sheet and a noble metal nanometer enzyme loaded on the Mo-doped WO 3 nanometer sheet.
  2. 2. The composite nanomaterial of claim 1, wherein the Mo is doped with WO 3 nanoplatelets having a Mo content of 3 to 30wt%, based on the total weight of WO 3 in the nanoplatelets being 100%.
  3. 3. The composite nanomaterial of claim 1, wherein the noble metal nanoenzyme is present in an amount of 0.2% -4% by weight, based on 100% of the total weight of the core material a.
  4. 4. The composite nanomaterial of claim 1, wherein the stabilizer B is present in an amount of 1 to 20wt%, based on 100% of the total weight of the composite nanomaterial.
  5. 5. The composite nanomaterial of claim 1, wherein the Mo-doped WO 3 nanoplatelets have one or more characteristics selected from the group consisting of: i) The Zeta potential is-50 to-65 mV; ii) particle size 70-85nm; iii) The specific surface area is 80-100m 2 g -1 ; iv) the aperture is 2-5 nm; v) the DPBF degradation rate of MWO groups 1 is 50-60%; vi) band gap of 2.0-3.2 eV.
  6. 6. The composite nanomaterial of claim 1, wherein the core material a has one or more characteristics selected from the group consisting of: i) The Zeta potential is-45 to-60 mV; ii) particle size of 70-85nm; iii) The DPBF degradation rate is 60-70%; iv) band gap is 2.5-4.0 eV.
  7. 7. The composite nanomaterial of claim 1, wherein the composite nanomaterial has one or more characteristics selected from the group consisting of: i) The grain diameter in H 2 O is 80-90 nm; ii) the particle size in Fetal Bovine Serum (FBS) is 110-120 nm; iii) Particle size in DMEM medium (10% FBS) is 90-100 nm; iv) particle size of 95-115 nm in 1640 medium (10% FBS); v) Zeta potential in H 2 O is-40 to-50 mV; vi) Zeta potential in Normal Saline (NS), fetal Bovine Serum (FBS), DMEM medium (10% FBS) and 1640 medium (10% FBS) was-5 to-12 mV.
  8. 8. A method for preparing a composite nanomaterial, the method comprising the steps of: providing a core material A, and mixing the core material A with a stabilizer B dissolved in deionized water to prepare the composite nano material.
  9. 9. A composition, wherein the composition comprises: (i) The composite nanomaterial of claim 1, and (Ii) A pharmaceutically acceptable carrier.
  10. 10. The use of the composite nanomaterial of claim 1, for the preparation of a medicament for tumor treatment; The tumor is selected from breast cancer tumor, prostate cancer tumor, colorectal cancer tumor, pancreatic cancer tumor, cervical cancer tumor, brain cancer tumor, lung cancer tumor, head and neck cancer, bile duct cancer, and gastric cancer.

Description

Composite nano material and preparation and application thereof Technical Field The invention relates to the technical field of medicines. In particular to a composite nano material, and preparation and application thereof. Background Malignant tumors are one of the main causes of death of human beings worldwide, and cause great physical and psychological stress and life threat to patients. Dynamic therapeutic strategies based on Reactive Oxygen Species (ROS) have received considerable attention because of their ability to induce oxidative stress in cancer cells, thereby triggering specific death of cancer cells. This strategy encompasses a variety of therapeutic approaches such as radiation power therapy (RDT), chemotherapy (CDT), photodynamic therapy (PDT), and sonodynamic therapy (SDT). Among these treatments, SDT has shown great potential in clinical applications due to its non-invasive and high tissue penetration characteristics. The therapy activates the sonosensitizer by ultrasonic wave to generate ROS at the tumor part, thereby achieving the purpose of treatment. In experimental studies of various tumor models, sonodynamic therapy has demonstrated good application effects and therapeutic properties. Although SDT is favored for its unique advantages, its therapeutic efficacy is still limited by a number of factors. First, the efficacy of the sonosensitizer is critical to the success of SDT. However, the solubility of the organic sonosensitizers widely used at present in physiological environments is low, chemical stability is poor, and phototoxicity duration is long, which severely limits their application in clinical practice. In contrast, inorganic sonosensitizers such as titanium oxide exhibit better stability and lower phototoxicity, but their ROS generation efficiency is relatively low, mainly due to their wide-band characteristics and rapid recombination of holes and electrons. Finally, tumor microenvironments contain higher levels of GSH, which can consume ROS, thereby diminishing the efficacy of ROS kinetic therapy. Accordingly, there is an urgent need in the art to develop a composite nanomaterial that can efficiently generate ROS while consuming GSH, and combine photodynamic therapy (SDT) with enzyme-catalyzed (ECT) therapy, which has less cytotoxicity and significant antitumor activity. Disclosure of Invention The invention aims to provide a composite nano material which can realize the generation of a large amount of ROS, effectively consume GSH and enhance the curative effect by combining enzyme catalytic treatment and photodynamic treatment means. In a first aspect of the present invention, there is provided a composite nanomaterial comprising a core material a and a stabilizer B; Wherein, the core material A comprises a Mo-doped WO 3 nanometer sheet and a noble metal nanometer enzyme loaded on the Mo-doped WO 3 nanometer sheet. In another preferred example, the noble metal in the noble metal nano-enzyme is selected from the group consisting of Pt, pd, au, or a combination thereof. In another preferred example, the noble metal nano-enzyme is Pt nano-enzyme. In another preferred embodiment, the stabilizer B is selected from the group consisting of polyvinylpyrrolidone (PVP), polyethylene glycol, hyaluronic acid, or a combination thereof. In another preferred embodiment, the stabilizer B is polyvinylpyrrolidone (PVP). In another preferred embodiment, the Mo-doped WO 3 nanoplatelets are sensitizers. In another preferred embodiment, the noble metal nanoenzyme is a monoatomic nanoenzyme. In another preferred embodiment, the Mo-doped WO 3 nanoplatelets have a Mo content of 3-30wt%, based on the total weight of WO 3 in the nanoplatelets being 100%. In another preferred embodiment, the content of the noble metal nano-enzyme is 0.2 to 4wt%, based on 100% of the total weight of the core material A. In another preferred example, the loading amount of the noble metal in the core material a is 0.2-5 atom%. In another preferred example, the content of the stabilizer B is 1-20wt%, and the content is calculated by 100% of the total weight of the composite nano material. In another preferred embodiment, the Mo-doped WO 3 nanoplatelets have one or more features selected from the group consisting of: i) The Zeta potential is-50 to-65 mV, preferably-55 to-65 mV, and more preferably-55 to-60 mV; ii) particle size of 70-85nm, preferably 75-85nm, more preferably 75-80nm; iii) The specific surface area is 80-100m 2 g-1, preferably 85-98m 2 g-1, more preferably 88-95m 2 g-1; iv) the pore diameter is 2-5 nm, preferably 3-5 nm; v) MWO groups of DPBF degradation of 50-60%, preferably 50-55%, more preferably 51-55%; vi) band gap of 2.0 to 3.2eV, preferably 2.0 to 3.0eV, more preferably 2.2 to 2.5eV. In another preferred embodiment, the core material a has one or more characteristics selected from the group consisting of: i) The Zeta potential is-45 to-60 mV, preferably-45 to-55 mV, more preferably-45 to-50 mV; ii)