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CN-121987840-A - Calcium ion zeolite imidazole skeleton composite hemostatic dressing, and preparation method and application thereof

CN121987840ACN 121987840 ACN121987840 ACN 121987840ACN-121987840-A

Abstract

The invention relates to a calcium ion zeolite imidazole skeleton composite hemostatic dressing, and a preparation method and application thereof, and belongs to the field of biomedical materials. Aims at solving the technical problems of low blood coagulation efficiency and single hemostatic effect of the existing hemostatic material. The composite hemostatic dressing comprises a cotton fiber substrate and Ca-ZIF-8 nano particles loaded on the surface and fiber gaps of the cotton fiber substrate. Ca 2+ is doped into a ZIF-8 framework and is loaded on cotton fibers in an in-situ growth mode, and the ZIF-8 porous structure has dual mechanisms of physical adsorption and physiological coagulation acceleration, namely, the ZIF-8 porous structure rapidly adsorbs blood plasma moisture enriched platelets and coagulation factors, and Ca 2+ is used as the coagulation factor IV to activate coagulation cascade reaction, and the two synergistically act. The preparation method is simple, the cost is controllable, the obtained dressing has stable structure and good biocompatibility, the blood coagulation index of an in-vitro coagulation experiment is close to 1%, and the dressing is suitable for incompressible bleeding scenes.

Inventors

  • ZOU YANG
  • WANG NAN
  • LV YONGGANG

Assignees

  • 武汉纺织大学

Dates

Publication Date
20260508
Application Date
20260402

Claims (9)

  1. 1. The calcium ion zeolite imidazole skeleton composite hemostatic dressing is characterized by comprising a cotton fiber substrate and Ca-ZIF-8 nano particles loaded on the surface of the cotton fiber substrate and in gaps among cotton fibers, wherein calcium ions are used as a blood coagulation factor IV to be doped into the ZIF-8 skeleton to form the Ca-ZIF-8 nano particles, and the Ca-ZIF-8 nano particles are rhombic dodecahedron and grow on the cotton fiber substrate in situ.
  2. 2. The calcium ion zeolite imidazole skeleton composite hemostatic dressing of claim 1, wherein the molar ratio of zinc ions to calcium ions in the Ca-ZIF-8 nanoparticles is 1:1.5-1:0.5.
  3. 3. The method for preparing the calcium ion zeolite imidazole skeleton composite hemostatic dressing according to claim 1 or 2, wherein the preparation method comprises the following steps: S1, dissolving zinc nitrate hexahydrate and anhydrous calcium chloride in methanol to obtain a metal salt precursor mixed solution; S2, immersing the cotton fiber substrate into the metal salt precursor mixed solution prepared in the step S1 after alkali liquor activation treatment, stirring at room temperature to coordinate and combine metal ions, and then adding 2-methylimidazole for standing reaction; And S3, taking out the cotton fiber in the step S2, filtering and washing the cotton fiber, and drying the cotton fiber in vacuum to obtain the calcium ion zeolite imidazole skeleton composite hemostatic dressing.
  4. 4. The method of claim 3, wherein the molar ratio of zinc ions to calcium ions in the metal salt precursor mixed solution obtained in the step S1 is 1:1.5-1:0.5.
  5. 5. The method according to claim 3 or 4, wherein in the step S2, the metal salt precursor mixture contains 30-60 wt% of metal salt and 1:14-1:16 of metal salt and 2-methylimidazole.
  6. 6. The method according to any one of claims 3 to 5, wherein in the step S2, the stirring speed is 300-500 r/min, the duration is 4-5 h, and the standing reaction duration is 24 h.
  7. 7. The method according to any one of claims 3 to 6, wherein in the step S3, the vacuum drying temperature is 50 ℃ and the duration is 8 to 12 hours.
  8. 8. Use of a method of preparation according to any one of claims 4-7 for the preparation of a hemostatic product.
  9. 9. The use according to claim 8, wherein the hemostatic product is for incompressible bleeding, such as post-liver puncture bleeding, deep wound bleeding, etc.

Description

Calcium ion zeolite imidazole skeleton composite hemostatic dressing, and preparation method and application thereof Technical Field The invention belongs to the field of biomedical materials, and relates to a calcium ion zeolite imidazole skeleton composite hemostatic dressing, and a preparation method and application thereof. Background Uncontrolled bleeding is a major cause of death in traumatized patients in war, surgery, and accidents. The high-efficiency hemostatic material is rapidly applied in the early stage of bleeding, so that the rescue window period can be effectively prolonged, and the death rate caused by excessive blood loss can be reduced. Therefore, the development of rapid and safe hemostatic materials has important clinical significance. The existing hemostatic materials are mainly divided into four types of natural polymers, synthetic polymers, inorganic materials and metal-containing materials. Although natural polymers (such as chitosan and cellulose) have good biocompatibility, the mechanical strength is low, the structural stability is poor, the supporting requirement of complex wounds is difficult to meet, synthetic polymers can be used for rapidly binding wound surfaces, but lack biological activity and can not actively activate a coagulation path, and inorganic materials (such as zeolite and kaolin) have stronger water absorption and coagulation promoting capability by virtue of a porous structure, but have potential safety hazards such as metabolic toxicity, thrombus risk and the like. Metal ions have unique advantages in hemostasis. Calcium ion (Ca 2+) is used as a blood coagulation factor IV, can activate the blood coagulation factor in the blood coagulation cascade reaction and promote platelet aggregation, and is a core factor of the physiological blood coagulation process. However, how to efficiently and stably load calcium ions in hemostatic materials and realize the controlled release thereof remains a difficulty of current researches. Disclosure of Invention In view of the above, the invention aims to provide a calcium ion zeolite imidazole skeleton composite hemostatic dressing, and a preparation method and application thereof. According to the invention, ca2 + is doped into a ZIF-8 framework, and a hemostatic material with a physical coagulation and physiological coagulation dual mechanism is constructed through the synergistic effect of the porous adsorption characteristic of the ZIF-8 and the physiological coagulation function of Ca2 +. In order to achieve the above purpose, the present invention provides the following technical solutions: The invention provides a calcium ion zeolite imidazole skeleton composite hemostatic dressing which comprises a cotton fiber substrate and Ca-ZIF-8 nano particles loaded on the surface of the cotton fiber substrate and in gaps among cotton fibers, wherein calcium ions are used as a blood coagulation factor IV to be doped into a ZIF-8 skeleton to form the Ca-ZIF-8 nano particles, and the Ca-ZIF-8 nano particles are rhombic dodecahedron and grow on the cotton fiber substrate in situ. Preferably, the molar ratio of zinc ions to calcium ions in the Ca-ZIF-8 nano particles is 1:1.5-1:0.5. Further, the invention also provides a preparation method of the calcium ion zeolite imidazole skeleton composite hemostatic dressing, which comprises the following steps: S1, dissolving zinc nitrate hexahydrate and anhydrous calcium chloride in methanol to obtain a metal salt precursor mixed solution; S2, immersing the cotton fiber substrate into the metal salt precursor mixed solution prepared in the step S1 after alkali liquor activation treatment, stirring at room temperature to coordinate and combine metal ions, and then adding 2-methylimidazole for standing reaction; And S3, taking out the cotton fiber in the step S2, filtering and washing the cotton fiber, and drying the cotton fiber in vacuum to obtain the calcium ion zeolite imidazole skeleton composite hemostatic dressing. Preferably, in the metal salt precursor mixed solution obtained in the step S1, the molar ratio of zinc ions to calcium ions is 1:1.5-1:0.5. Preferably, in the step S2, the mass ratio of the metal salt contained in the metal salt precursor mixed solution to the cotton fiber is 30-60 wt%, and the molar ratio of the metal salt to the 2-methylimidazole is 1:14-1:16. Preferably, in the step S2, the stirring speed is 300-500 r/min, the duration is 4-5 h, and the standing reaction duration is 24 h. Preferably, in step S3, the vacuum drying temperature is 50 ℃ and the duration is 8-12 hours. Further, the invention also provides application of the preparation method of the calcium ion zeolite imidazole skeleton composite hemostatic dressing in preparing hemostatic products; Preferably, the hemostatic product is used for incompressible bleeding, such as post-liver puncture bleeding, deep wound bleeding, and the like. The invention has the beneficial effects that: 1. Synergistic effe