Search

CN-121987841-A - Biological polysaccharide wound dressing with effects of diminishing inflammation, promoting healing and reducing scars and preparation method thereof

CN121987841ACN 121987841 ACN121987841 ACN 121987841ACN-121987841-A

Abstract

The invention discloses a biological polysaccharide wound dressing with the effects of diminishing inflammation, promoting healing and reducing scars and a preparation method thereof; the dressing takes chitosan, sodium hyaluronate and the like as matrixes, is compounded with modified biological polysaccharide and active composite auxiliary agent, and constructs a three-dimensional network structure through a biological crosslinking agent, the preparation process adopts mild conditions such as isopropanol-water mixed solvent, low-temperature alkalization and the like, is green and environment-friendly, and experiments show that the dressing can realize synergistic effects of diminishing inflammation, promoting healing and reducing scars, has excellent biocompatibility and stable mechanical property, can obviously shorten wound healing time, reduces scar proliferation rate, and is suitable for repairing various wound surfaces such as burns, chronic ulcers and the like.

Inventors

  • SUN PENGCHENG
  • WEN LIEMING
  • LI JUAN
  • Sun Taoli
  • WU JUNYONG
  • YANG SHUYI
  • JIANG QIULIAN

Assignees

  • 湖南微肽生物医药有限公司

Dates

Publication Date
20260508
Application Date
20260209

Claims (10)

  1. 1. A biological polysaccharide wound dressing with the effects of diminishing inflammation, promoting healing and reducing scars is characterized by comprising the following components, by weight, 20-40 parts of matrix components, 15-30 parts of modified biological polysaccharide, 10-25 parts of biological cross-linking agents and 7-12 parts of active compound auxiliary agents.
  2. 2. The biological polysaccharide wound dressing according to claim 1, wherein the matrix component is a mixture of chitosan, sodium hyaluronate, sodium alginate, carbomer and xanthan gum, and the mass ratio of the matrix component is (3-5): (2-4): (1-3): (0.5-1.5): (0.3-0.8).
  3. 3. The biological polysaccharide wound dressing according to claim 1, wherein the modified biological polysaccharide is modified chitosan and oxidized sodium alginate in a mass ratio of 1:0.3-0.9.
  4. 4. A biological polysaccharide wound dressing according to claim 3, wherein the method of preparing the modified chitosan comprises the steps of: A1, dispersing chitosan powder in a mixed solvent consisting of isopropanol and deionized water according to a volume ratio (8-12): 1, wherein the dispersion mass volume ratio g/mL is 1:5-10, slowly dropwise adding a 40-50wt% sodium hydroxide aqueous solution under the conditions of ice-water bath and stirring at 0-5 ℃, and carrying out an alkalization reaction for 2-4 hours to obtain an alkalized chitosan suspension; And A2, adding the sodium chloroacetate aqueous solution into the alkalized chitosan suspension, wherein the molar ratio of chitosan to sodium chloroacetate is 1 (2-3), reacting for 3-5 hours at 50-60 ℃, and neutralizing, filtering, washing and drying to obtain the modified chitosan.
  5. 5. A biological polysaccharide wound dressing according to claim 3, wherein the preparation method of the oxidized sodium alginate comprises the following steps: B1, dispersing sodium alginate in a mixed solvent consisting of ethanol and deionized water according to the volume ratio of (4-6): 1, and uniformly stirring to prepare a suspension with the concentration of 20-50 mg/mL; under the condition of avoiding light, dripping 0.5-1.0mol/L sodium periodate aqueous solution into the suspension, and carrying out light-free oxidation reaction for 2-8 hours under the condition of 10-30℃, pH < 3 > -5, wherein the volume ratio of the sodium periodate aqueous solution to the sodium alginate suspension is (0.5-1.5): 1; and B3, after the reaction is finished, adding ethylene glycol to terminate the reaction, and then dialyzing, purifying and freeze-drying to obtain oxidized sodium alginate.
  6. 6. The biopolysaccharide wound dressing according to claim 1 wherein the biological cross-linking agent is genipin.
  7. 7. The biological polysaccharide wound dressing according to claim 1, wherein the active composite auxiliary agent is a mixture of dencichine, beta-glucan and escin, and the mass ratio is (1-2) (0.5-1) (0.3-0.8).
  8. 8. The biological polysaccharide wound dressing according to claim 1, further comprising adjuvants of 0.5-1.5 parts by weight of carbomer, 0.3-0.8 parts by weight of xanthan gum, 0.1-0.5 parts by weight of triethanolamine, 2-4 parts by weight of butanediol, 4-8 parts by weight of glycerol, 0.15-0.4 parts by weight of p-hydroxyacetophenone, 0.15-0.4 parts by weight of 1, 2-hexanediol, 0.05-0.15 parts by weight of disodium edetate and 100-150 parts by weight of purified water.
  9. 9. A method of preparing a biological polysaccharide wound dressing according to any one of claims 1 to 8, comprising the steps of: S1, preparing a mixed solution, namely mixing matrix components and modified polysaccharide, adding purified water and disodium ethylenediamine tetraacetate, stirring and dissolving at 40-50 ℃, sequentially adding butanediol, glycerol, p-hydroxyacetophenone and 1, 2-hexanediol, stirring for 30-60min, adding active composite auxiliary agent, and continuously stirring for 1-2h until uniformity to obtain the mixed solution; S2, crosslinking reaction, namely dripping a genipin water solution into the mixed solution, regulating the pH of the system to 5.5-6.5 by using triethanolamine, and crosslinking for 2-4 hours at a constant temperature of 30-40 ℃; s3, molding and post-treatment, namely injecting the crosslinked mixture into a mold, freeze-drying to obtain a porous dressing blank, soaking the blank in 0.1-0.5% v/v glycerol aqueous solution for 10-20min, taking out, and vacuum-drying to obtain the wound dressing.
  10. 10. The method of claim 9, wherein, The stirring speed in the step S1 is 300-500rpm; The mass concentration of the genipin aqueous solution in the step S2 is 5-10%, and the dropping speed is 0.5-1mL/min; The condition of freeze drying in the step S3 is that pre-freezing is carried out for 2-3 hours at the temperature of minus 20 plus or minus 3 ℃, and then freeze drying is carried out for 12-18 hours at the temperature of minus 50 plus or minus 5 ℃ and the vacuum degree of less than or equal to 10 Pa.

Description

Biological polysaccharide wound dressing with effects of diminishing inflammation, promoting healing and reducing scars and preparation method thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to a biological polysaccharide wound dressing with the effects of diminishing inflammation, promoting healing and reducing scars and a preparation method thereof. Background Skin wound healing is a complex biological process involving four overlapping and delicate stages of hemostasis, inflammation, proliferation and tissue remodeling. Among these, effective regulation of the inflammatory phase is critical in determining the quality and rate of healing. Excessive or sustained inflammatory response not only exacerbates tissue damage and delays re-epithelialization, but also results in a major contributor to pathological collagen overdose and hypertrophic scar formation. At present, the traditional wound dressing such as gauze, vaseline oil gauze and the like have the limitations of poor biocompatibility, easy adhesion to the wound surface, incapability of effectively promoting healing, scar prevention and the like. In recent years, biological polysaccharide dressing has good biodegradability, moisture retention and biological activity, and becomes a research hot spot in the field of wound repair. However, the existing biological polysaccharide dressing still has the defects that the single polysaccharide component has single function, is difficult to simultaneously meet the comprehensive requirements of diminishing inflammation, promoting healing and reducing scars, the partial polysaccharide material has poor water solubility and insufficient mechanical property, limits the application of the polysaccharide material to complex wound surfaces, is difficult to regulate and control the release rate of active ingredients, easily causes the local concentration to be too high or too low, influences the repair effect, has the hidden danger of biocompatibility and possibly causes the irritation or anaphylactic reaction of the wound surfaces. Therefore, the biological polysaccharide wound dressing which has the advantages of multiple effects, synergy, excellent biocompatibility, stable structure and green preparation process is developed, and has important significance for improving the wound repair quality and reducing scar formation. Disclosure of Invention In view of the above, the present invention provides a biological polysaccharide wound dressing with anti-inflammatory, healing promoting and scar reducing effects and a preparation method thereof, which solve the above problems. The biological polysaccharide wound dressing with the effects of diminishing inflammation, promoting healing and reducing scars is realized by the technical scheme that the biological polysaccharide wound dressing comprises, by weight, 20-40 parts of matrix components, 15-30 parts of modified biological polysaccharide, 10-25 parts of biological crosslinking agents and 7-12 parts of active compound auxiliary agents. Preferably, the matrix component is a mixture of chitosan, sodium hyaluronate, sodium alginate, carbomer and xanthan gum, and the mass ratio of the matrix component is (3-5): (2-4): (1-3): (0.5-1.5): (0.3-0.8). Preferably, the modified biological polysaccharide is modified chitosan and oxidized sodium alginate with a mass ratio of 1:0.3-0.9. Preferably, the preparation method of the modified chitosan comprises the following steps: A1, dispersing chitosan powder in a mixed solvent consisting of isopropanol and deionized water according to a volume ratio (8-12): 1, wherein the dispersion mass volume ratio g/mL is 1:5-10, slowly dropwise adding a 40-50wt% sodium hydroxide aqueous solution under the conditions of ice-water bath and stirring at 0-5 ℃, and carrying out an alkalization reaction for 2-4 hours to obtain an alkalized chitosan suspension; A2, adding the sodium chloroacetate aqueous solution into the alkalized chitosan suspension, wherein the molar ratio of chitosan to sodium chloroacetate is 1 (2-3), reacting for 3-5 hours at 50-60 ℃, and neutralizing, filtering, washing and drying to obtain the modified chitosan, wherein the substitution degree of carboxymethyl is 0.6-0.9. Preferably, the preparation method of the oxidized sodium alginate comprises the following steps: B1, dispersing sodium alginate in a mixed solvent consisting of ethanol and deionized water according to the volume ratio of (4-6): 1, and uniformly stirring to prepare a suspension with the concentration of 20-50 mg/mL; under the condition of avoiding light, dripping 0.5-1.0mol/L sodium periodate aqueous solution into the suspension, and carrying out light-free oxidation reaction for 2-8 hours under the condition of 10-30℃, pH < 3 > -5, wherein the volume ratio of the sodium periodate aqueous solution to the sodium alginate suspension is (0.5-1.5): 1; and B3, after the reaction is finished, adding ethylene