CN-121987843-A - Hydrogel dressing loaded with hemoglobin crystals and application thereof

Abstract

The invention relates to the technical field of biology, and particularly discloses a hydrogel dressing loaded with hemoglobin crystals and application thereof. The hydrogel dressing is prepared by uniformly mixing N-acryloylglycine amide, acrylamide and hemoglobin crystals, and then crosslinking the mixture into gel under the action of a photoinitiator and a photocrosslinker by ultraviolet light, wherein the N-acryloylglycine amide and the acrylamide undergo free radical polymerization reaction to form a copolymer with a three-dimensional network structure, and the hemoglobin crystals are embedded into the three-dimensional network structure to obtain the hydrogel dressing. The hydrogel dressing provided by the invention can respond to the local anoxic microenvironment of the wound, release oxygen continuously, relieve tissue hypoxia and exert an immunoregulatory effect, thereby effectively promoting the healing of the skin wound. The hydrogel provided by the invention has excellent biocompatibility and clinical applicability, can obviously promote migration, proliferation and differentiation of skin fibroblasts, and provides a high-efficiency and safe solution for repairing skin wound surfaces.

Inventors

• WEI QINGHUA
• AN YALONG
• WANG XINGLI
• ZHOU RENBIN
• CUI NING

Assignees

• 西北工业大学

Dates

Publication Date

20260508

Application Date

20260311

Claims (10)

1. 1. A hydrogel dressing loaded with hemoglobin crystals is characterized in that N-acryloylglycine amide, acrylamide and hemoglobin crystals are uniformly mixed and then crosslinked into gel under the action of a photoinitiator and a photocrosslinker, wherein the N-acryloylglycine amide and the acrylamide undergo free radical polymerization reaction to form a copolymer with a three-dimensional network structure, and the hemoglobin crystals are embedded into the three-dimensional network structure of the copolymer to obtain the hydrogel dressing.
2. 2. The hydrogel dressing of claim 1, wherein the mass ratio of N-acryloylglycinamide to acrylamide is 1-4:1.
3. 3. The hydrogel dressing of claim 1, wherein the hemoglobin crystals have a final mass concentration in the system of 1% -5%.
4. 4. The hydrogel dressing of claim 1, wherein the photoinitiator is a LAP solution having a mass concentration of 0.1% -0.5%.
5. 5. The hydrogel dressing of claim 1, wherein the photocrosslinker is polyethylene glycol diacrylate and the molar ratio of photocrosslinker to acrylamide is 1:20-100.
6. 6. The hydrogel dressing of claim 5, wherein the polyethylene glycol diacrylate is added while stirring under nitrogen until completely dissolved.
7. 7. The hydrogel dressing of claim 1, wherein the ultraviolet light used for ultraviolet crosslinking has a wavelength of 365nm to 405nm and a light intensity of 15mW +. ~20mW/ The irradiation time is 10 s-30 s.
8. 8. The hydrogel dressing of claim 1, wherein the hemoglobin crystals are prepared by the steps of: dissolving hemoglobin in PBS, preparing hemoglobin solution, centrifuging, and collecting supernatant; adding polyethylene glycol into the supernatant, standing to precipitate, aggregate and precipitate hemoglobin; washing the obtained precipitate, adding into glutaraldehyde solution, standing overnight, centrifuging, and discarding supernatant to obtain hemoglobin crystal.
9. 9. The hydrogel dressing of claim 8, wherein the mass concentration of polyethylene glycol is 50%, the volume ratio of the supernatant to the polyethylene glycol solution is 1:1, and the mass concentration of glutaraldehyde solution is 0.5% -2%.
10. 10. Use of a hydrogel dressing according to claims 1-9 for the preparation of a medical dressing for promoting healing of skin wounds.

Description

Hydrogel dressing loaded with hemoglobin crystals and application thereof Technical Field The invention relates to the technical field of material preparation, in particular to a hydrogel dressing with a continuous oxygen release function for loading hemoglobin crystals and application thereof. Background Skin is the most fragile organ of human body, and is always the most effective barrier for maintaining stable environment in the body and resisting external severe environment. As such, skin surfaces often have wounds, such as mechanical injury, burns, surgical wounds, etc., the healing of which has been a major challenge, not only affecting the patient's postoperative recovery, probability of disease recurrence, but also affecting the patient's normal life. Although skin wound management is now well established, millions of people are exposed to prolonged skin tissue repair times each year due to wound infection and dehiscence. Wound healing is a dynamic and tightly regulated process by growth factors, cytokines, including four phases of hemostasis, inflammation, proliferation and remodeling. Among many factors, oxygen is considered a key regulator affecting these stages. In the early stages of wound healing, transient hypoxia activates signal pathways, stimulating keratinocyte proliferation, and transmitting angiogenic signals. However, when hypoxia continues to the inflammatory phase, hypoxia impairs fibroblast activity, inhibits collagen synthesis, destroys neovascularization, and exacerbates pro-inflammatory cytokine production, prolonging the inflammatory phase, where hypoxia can instead cause a barrier to wound repair. Thus, increasing the oxygen level at the wound tissue is an important means of accelerating the healing of skin wounds. At present, the treatment of skin wounds mainly depends on non-adhesive dressing containing Vaseline, and although the non-adhesive dressing can provide breathable and moisturizing environment for the wounds, the biological environment for wound healing is limited, serious inflammatory reaction is difficult to inhibit, and the difficult problem of wound healing of long-term hypoxia cannot be solved. Thus, there is a great clinical need for advanced wound care techniques that can regulate the wound microenvironment by restoring oxygen balance, alleviating inflammation, and promoting tissue regeneration. Hydrogels with three-dimensional networks have been receiving attention from researchers due to their high water content, adjustable structure, and excellent biocompatibility, which enable them to maintain moist at the wound site, absorb tissue exudates, and mimic the microenvironment of the cytoplasmic matrix. At present, the traditional hydrogel dressing on the market cannot achieve the expected treatment effect on irregular shapes due to weak tissue adhesion and insufficient functionality. Therefore, it is very important to develop a hydrogel dressing with strong adhesion, toughness, excellent mechanical property and good biocompatibility. A variety of hypoxia regulated skin dressings have been developed to improve wound healing by continuous oxygenation in hypoxic environments. Most of these dressings are based on peroxides such as H 2O2、CaO2. A novel oxygen-generating tissue scaffold with predictable oxygen release kinetics was developed as Suvarnapathaki et al, which enhanced the ability of gelatin methacryloyl to release oxygen continuously in vitro and in vivo by CaO 2 -PCL microparticles, thereby promoting tissue remodeling and regeneration. In addition, poplar et al developed a nano-oxygen-containing gel containing perfluorosodium Carlin-coated albumin nanoparticles, called nano-oxygen-containing powder, capable of locally delivering dissolved oxygen to the wound surface, thereby achieving wound repair. However, these oxygenation strategies are basically oxygen release by chemical substances, and it is difficult to overcome the problem of cytotoxicity, and at the same time, the oxygen release system is difficult to control, and the oxygen release time and total amount are difficult to regulate. Therefore, the development of a skin dressing which has excellent biocompatibility, can continuously release oxygen and regulate the oxygen release time and the oxygen release amount is also very important. Disclosure of Invention Aiming at the problems that the prior hydrogel skin dressing has weak mechanical property, poor biocompatibility and difficult control of an oxygen release system, the invention provides the hemoglobin crystal-loaded hydrogel dressing with the continuous oxygen release function, and the components of the hydrogel dressing are nontoxic and have no side effect, have good biocompatibility and stability, do not have immune rejection and cytotoxicity, and can be used as a skin repair regeneration material for repairing skin wounds. The invention is realized by the following technical scheme: The invention provides a hydrogel dressing loaded with h