CN-121987845-A - Magnesium diboride nano-enzyme with ROS quenching effect and application thereof

Abstract

The invention relates to the technical field of bone repair, in particular to magnesium diboride nano-enzyme with ROS quenching effect and application thereof. The invention provides a nano material MgB 2 with high activity of dehydrogenase, good biological safety and excellent chemical stability. The compound can not only remove free radicals in vitro, but also remove free radicals in cells at the same high efficiency at the cellular level, and reduce oxidative stress damage. The advantages can be expanded to the fields of multi-bone repair and bone regeneration.

Inventors

• GAO MENG
• ZHENG HUIZHEN
• CHEN CHANGZHI

Assignees

• 苏州大学

Dates

Publication Date

20260508

Application Date

20260130

Claims (10)

1. 1. The preparation method of the magnesium diboride nano-enzyme with the ROS quenching effect is characterized by comprising the following steps of: s11, sintering magnesium and boron at 650-900 ℃ to obtain nano magnesium diboride, wherein the temperature of the magnesium and the boron is 1-48 h; and S12, wrapping the nano magnesium diboride in gel to obtain the magnesium diboride nano enzyme.
2. 2. The method of claim 1, wherein the molar ratio of magnesium to boron is 1:1-3.
3. 3. The method according to claim 1, wherein the nano magnesium diboride has a thickness of 0.1 to 5 nm and a size of 20 to 500 nm.
4. 4. The method of claim 1, wherein the nano magnesium diboride is present in the gel at a concentration of 0.01 to 50 μg/mL.
5. 5. The method according to claim 1, wherein the gel is made of gelatin, hydroxypropyl methylcellulose, alginate, collagen, hyaluronic acid, polyvinyl alcohol, polyethylene glycol or chitosan.
6. 6. A magnesium diboride nanoenzyme with ROS quenching effect prepared by the preparation method of any one of claims 1 to 5.
7. 7. The use of magnesium diboride nanoenzyme with ROS quenching effect of claim 6 for bone regeneration or bone repair.
8. 8. Use of magnesium diboride nanoenzyme with ROS quenching effect according to claim 7 for bone regeneration or bone repair, wherein the temperature at the time of application is 0-60 ℃ for 1 second-96 hours.
9. 9. Use of magnesium diboride nanoenzyme with ROS quenching effect according to claim 7 for bone regeneration or bone repair, wherein the cell type of the use comprises epithelial cells, immune cells, connective tissue cells, muscle cells or cells of the nervous system.
10. 10. A bone repair implant comprising a magnesium diboride nanoenzyme having ROS quenching effect according to claim 6.

Description

Magnesium diboride nano-enzyme with ROS quenching effect and application thereof Technical Field The invention relates to the technical field of bone repair, in particular to magnesium diboride nano-enzyme with ROS quenching effect and application thereof. Background Oxidative stress is an inevitable phenomenon in the metabolic process of cells, and when Reactive Oxygen Species (ROS) (e.g., hydroxyl radical OH, superoxide anion O 2-, hydrogen peroxide H 2O2, etc.) are excessively accumulated, the redox balance of cells is destroyed, resulting in oxidative damage of biomacromolecules such as proteins, lipids, and DNA, and further induction of inflammatory reactions, apoptosis, and various diseases. In particular, in the field of bone regeneration and bone repair, excessive ROS can cause apoptosis of osteoblasts, inhibit transcription factors associated with osteogenic differentiation, and damage to oxidized collagen and bone matrix. Therefore, efficient removal of excess ROS is a key scientific problem in the field of bone regeneration and bone repair. Under physiological conditions, natural dehydrogenases, superoxide dismutases (SOD), catalases (CAT) and the like play a central role in regulating cell redox balance. However, these natural enzymes generally have problems of limited sources, high cost, easy inactivation, etc., and are difficult to meet the requirements of long-term storage and complex environmental application. In order to overcome the limitations of natural enzymes in stability and application environment, researchers in recent years develop a class of nanomaterials-nanoenzymes with enzyme-like activity. Compared with natural enzymes, the nano-enzyme has the advantages of low cost, high stability, simple preparation process, repeated use and the like, and therefore, the nano-enzyme has wide application prospect in the fields of biocatalysis, disease treatment, environmental purification and the like. The nano-enzyme reported at present mainly comprises metal oxide (such as CeO 2、Fe3O4), metal sulfide (such as MoS 2、Co3S4), carbon-based material (such as graphene and carbonitride) and the like, and the materials mainly show various bionic catalytic activities such as superoxide dismutase (SOD-like), peroxidase-like (POD-like) or catalase-like (CAT-like) activities and the like. However, nanomaterial studies directed to dehydrogenase-like activity remain relatively scarce. A small number of metal borides (e.g., moB 2) have been reported to possess dehydrogenase-like activity and to be capable of participating in the regulation of redox pairs (e.g., NAD +/NADH) in vivo. However, related research reports also indicate that MoB 2 may induce cellular reduction stress, and that biosafety presents a potential risk, limiting its further application in the field of bone repair. On the other hand, magnesium (Mg) element, which is an important constituent element of human bone tissue, has been widely studied and cited in the field of bone repair and bone regeneration. For example, ① in degradable Mg-based metal implant, mg element can be released gradually in vivo to promote fracture healing, ②Mg2+ doped biological ceramic material can improve the component similarity of the material and natural bone, enhance the adhesion and differentiation capacity of osteoblast, ③ Mg doped biphasic calcium phosphate material has been widely used for repairing large-area bone defects. The above studies fully show that magnesium has unique advantages in regulating the osteogenic microenvironment and promoting bone regeneration. Therefore, the nano material which has dehydrogenase-like activity and contains magnesium element is expected to play a synergistic effect in the aspects of removing excessive ROS, regulating and controlling local redox steady state, promoting bone regeneration, bone repair and the like, and has important research value and wide application prospect in the fields of bone repair and bone regeneration. It is explicitly reported at present that in the nano material with the dehydrogenase-like activity, for example, tin selenide (SnSe) nano material shows the lactate dehydrogenase-Like (LDH) activity, and can promote the conversion of lactic acid into pyruvic acid in cells, thereby regulating the metabolic balance of tumor microenvironment and playing an anti-tumor role. However, the material contains transition metal and selenium element, and has limited biological safety. It may gradually accumulate in metabolic organs (liver, kidney) over time. Also molybdenum boride (MoB 2) nanomaterials, which can lead to reduced stress in cells, are also at risk of biosafety (ACS appl. Mater. Interfaces 2022, 14, 24, 27651-27665 and Nature Nanotechnology 2025, 20, 554-562). Disclosure of Invention Therefore, the technical problem to be solved by the invention is that the currently reported dehydrogenase-like nanomaterial (such as SnSe and MoB 2) is difficult to be widely applied in the biomedical fields of int