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CN-121987850-A - Microcapsule coated with oil phase and preparation method and application thereof

CN121987850ACN 121987850 ACN121987850 ACN 121987850ACN-121987850-A

Abstract

The application provides a microcapsule for wrapping grease and a preparation method and application thereof. The application successfully obtains the hydrogel microcapsule coated with the grease, and fills the blank that the injection-grade product has no water-oil biphasic carrier. Furthermore, the hydrogel microcapsule coated with the grease, which is obtained by the application, has proper mechanical properties, and provides a referent raw material for developing subcutaneous slow-release agents or subcutaneous fillers in future.

Inventors

  • CHEN SHUNPING
  • LIU ZHE

Assignees

  • 华熙生物科技股份有限公司

Dates

Publication Date
20260508
Application Date
20260211

Claims (9)

  1. 1. A microcapsule coated with an oil phase comprises an oil phase and calcium alginate hydrogel for coating the oil phase, The hydrogel is prepared from hydroxyapatite and sodium alginate, Wherein the average particle size of the hydroxyapatite is 100 nm-800 nm, The molecular weight of the sodium alginate is 50 kDa-300 kDa, the content of the sodium alginate is 0.1-6 w/v%, The mass ratio of the hydroxyapatite to the sodium alginate is 1:30-1:6.
  2. 2. The microcapsule according to claim 1, wherein the mass ratio of hydroxyapatite to sodium alginate is 1:25 to 1:8.
  3. 3. The microcapsule according to claim 1 or 2, wherein the average particle diameter of the microcapsule is 100 to 500 μm.
  4. 4. A microcapsule according to claim 1, wherein the sodium alginate is present in an amount of 1 to 6w/v%.
  5. 5. The microcapsule according to any one of claims 1-4, wherein the oil phase comprises one or more oily substances.
  6. 6. A method of preparing the microcapsule of any one of claims 1-5, comprising the steps of: The oil phase, the intermediate phase and the outer phase are formed into the microcapsule through a coaxial three-phase microfluidic device, The oil phase comprises one or more oily substances, The intermediate phase comprises hydroxyapatite and sodium alginate which are uniformly dispersed in water, wherein the average particle size of the hydroxyapatite is 100 nm-800 nm, the molecular weight of the sodium alginate is 50 kDa-300 kDa, the content of the sodium alginate is 0.1w/v% -6 w/v%, the mass ratio of the hydroxyapatite to the sodium alginate is 1:30-1:6, The external phase is a hydrophobic solvent containing an organic acid.
  7. 7. The production method according to claim 6, wherein the content of the organic acid in the external phase is 1 to 5 wt%.
  8. 8. The method of making according to claim 6 or 7, wherein the intermediate phase is contacted with the outer phase to solidify to form a calcium alginate hydrogel.
  9. 9. Use of the oil-phase-coated microcapsules according to any one of claims 1 to 5 or the oil-phase-coated microcapsules prepared by the preparation method according to any one of claims 6 to 8 for the preparation of subcutaneous sustained release agents or subcutaneous fillers.

Description

Microcapsule coated with oil phase and preparation method and application thereof Technical Field The application belongs to the technical field of materials, and particularly relates to a microcapsule coated with an oil phase, and a preparation method and application thereof. Background At present, the medical filler market is accelerating iteration from single water phase filling to water-oil two-phase filling, and the trend accurately meets the dual requirements of consumers on instant shaping and long-acting nature. However, the current biphasic filling technology faces the problem of insufficient stability of compatibility of water and oil phases, and the industry generally adopts an operation mode of temporary mixing before injection, but mechanical extrusion, tissue fluid infiltration and aggregation generated by repeated movement of the subcutaneous environment after implantation still can lead to rapid segregation of the water and oil phases, so that not only is the molding effect poor, the maintenance time shortened, but also local edema, displacement accumulation and other steamed bread risks possibly caused. Under the background, the oil-in-water structure of the oil-in-water coating by utilizing the hydrogel microcapsule is a reliable technical route, and the cooperative delivery and controllable release of the water-oil phase can be realized by constructing a stable biphasic carrier. However, hydrogel microcapsules suitable for use in medical fillers typically have a particle size of 50-1000 μm, and the ability to successfully obtain lipid-encapsulated hydrogel microcapsules presents a significant challenge compared to lipid-free hydrogel particles. Disclosure of Invention Aiming at the problems existing in the prior art, the inventor of the application discovers that the microcapsule which coats the oil phase can be successfully formed stably by controlling the grain size and content of hydroxyapatite (CaHA) and the molecular weight and content of Sodium Alginate (SA), and the grain size meets the injection-grade requirement, thus providing a referent raw material for developing subcutaneous slow-release agents or subcutaneous fillers in the future. In particular, the application relates to the following aspects: 1. A microcapsule coated with an oil phase comprises an oil phase and calcium alginate hydrogel for coating the oil phase, The hydrogel is prepared from hydroxyapatite and sodium alginate, Wherein the average particle size of the hydroxyapatite is 100 nm-800 nm, The molecular weight of the sodium alginate is 50 kDa-300 kDa, the content of the sodium alginate is 0.1-6 w/v%, The mass ratio of the hydroxyapatite to the sodium alginate is 1:30-1:6. 2. The microcapsule according to item 1, wherein the mass ratio of hydroxyapatite to sodium alginate is 1:25 to 1:8. 3. The microcapsule according to item 1 or 2, wherein the average particle diameter of the microcapsule is 100 to 500 μm. 4. The microcapsule according to item 1, wherein the sodium alginate is present in an amount of 1w/v% -6 w/v%. 5. The microcapsule according to any one of claims 1-4, wherein the oil phase comprises one or more oily substances. 6. A method of preparing the microcapsule according to any one of claims 1-5, comprising the steps of: The oil phase, the intermediate phase and the outer phase are formed into the microcapsule through a coaxial three-phase microfluidic device, The oil phase comprises one or more oily substances, The intermediate phase comprises hydroxyapatite and sodium alginate which are uniformly dispersed in water, wherein the average particle size of the hydroxyapatite is 100 nm-800 nm, the molecular weight of the sodium alginate is 50 kDa-300 kDa, the content of the sodium alginate is 0.1w/v% -6 w/v%, the mass ratio of the hydroxyapatite to the sodium alginate is 1:30-1:6, The external phase is a hydrophobic solvent containing an organic acid. 7. The production method according to item 6, wherein the content of the organic acid in the external phase is 1 to 5% by weight. 8. The method of manufacture of item 6 or 7, wherein the intermediate phase is cured in contact with the outer phase to form a calcium alginate hydrogel. 9. The oil-phase-coated microcapsule according to any one of claims 1 to 5 or the oil-phase-coated microcapsule prepared by the preparation method according to any one of claims 6 to 8, for use in the preparation of subcutaneous sustained release agents or subcutaneous fillers. The beneficial effects of the application are that The application successfully obtains the hydrogel microcapsule coated with the grease, and fills the blank that the injection-grade product has no water-oil biphasic carrier. Furthermore, the hydrogel microcapsule coated with the grease, which is obtained by the application, has proper mechanical properties, and provides a referent raw material for developing subcutaneous slow-release agents or subcutaneous fillers in future. Drawings Fig. 1 is a schemati