CN-121987855-A - Osteogenesis nano-enzyme doped with metal ions and application thereof

Abstract

The invention relates to an osteogenic nano-enzyme doped with metal ions and application thereof, belonging to the technical field of biological medicine. According to the invention, the active metal ions are partially substituted for the iron ions in the Prussian blue nano enzyme crystal lattice by utilizing the open crystal structure characteristics of the Prussian blue nano enzyme, so that the metal ion doped osteogenic nano enzyme is prepared. The osteogenic nano-enzyme can also endow the material with a new bioactivity function on the premise of keeping the original catalytic performance of Prussian blue nano-enzyme, thereby accurately regulating the micro-environment of the diabetic bone and powerfully promoting the regeneration of bone tissues in a hyperglycemia environment. The osteogenic nano enzyme disclosed by the invention is integrated into the PCL bracket, so that the bracket has strong osteoinduction, antioxidation and anti-inflammatory functions, and a good foundation is laid for the application of the osteogenic nano enzyme in the regeneration of diabetic bones.

Inventors

• YANG CHEN
• ZENG ZHEN
• WANG YINGBO
• MENG ZHAOXU
• LUO KUI
• CHANG JIANG
• LI BIN

Assignees

• 苏州大学

Dates

Publication Date

20260508

Application Date

20260206

Claims (10)

1. 1. The osteogenic nano enzyme doped with metal ions is characterized by being prepared by mixing Prussian blue nano enzyme and metal ions under the existence of a surfactant and a stabilizer, performing a first reaction, adding potassium ferricyanide after the first reaction is finished, performing a second reaction, and freeze-drying to obtain the osteogenic nano enzyme.
2. 2. The osteogenic nanoenzyme of claim 1, wherein said metal ion is selected from one or more of Mg 2+ 、Mn 2+ 、Co 2+ 、Cu 2+ 、Zn 2+ 、Sr 2+ 、Mo 2+ and Eu 3+ .
3. 3. The osteogenic nanoenzyme of claim 1, wherein said stabilizer comprises trisodium citrate.
4. 4. The osteogenic nanoenzyme of claim 1, wherein, the surfactant comprises polyvinylpyrrolidone.
5. 5. Use of the osteogenic nanoenzyme of any one of claims 1-4 for the preparation of a bone repair material.
6. 6. Use of the osteogenic nanoenzyme of any one of claims 1-4 for the preparation of a product for repairing bone damage caused by type 2 diabetes.
7. 7. Use of the osteogenic nanoenzyme of any of claims 1-4 for the preparation of an antioxidant product.
8. 8. An osteogenic composite material, which is characterized in that, the osteogenic composite comprising the osteogenic nanoenzyme of any of claims 1-4 and a polycaprolactone.
9. 9. Use of the osteogenic composite of claim 8 for the preparation of a bone repair material.
10. 10. Use of the osteogenic composite of claim 8 for the preparation of a product for repairing bone damage caused by type 2 diabetes.

Description

Osteogenesis nano-enzyme doped with metal ions and application thereof Technical Field The invention relates to the technical field of biological medicine, in particular to an osteogenic nano-enzyme doped with metal ions and application thereof. Background Diabetes, a global metabolic disease, has a continuously rising incidence, and has become a major public health problem threatening human health. According to the latest statistics published by the international diabetes union (IDF) 2023, it is shown that the number of diabetics in adults 20-79 years old worldwide is over 5.3 hundred million, and it is expected that 2045 years will reach 7.8 hundred million, with a growth of 46%. Among the various complications of diabetes mellitus, lesions of the skeletal system are increasingly receiving attention from the academic community, in particular the problems of abnormal bone metabolism and bone regeneration disorders that result therefrom. A number of clinical epidemiological studies have demonstrated that diabetics are at significantly higher risk of developing fractures than non-diabetic individuals. Under the pathological conditions of diabetes, the regeneration efficiency of the traditional bone repair materials is obviously restricted, and researchers in recent years begin to explore novel functional bone repair materials. The ideal bone repair material needs to have good biocompatibility and mechanical properties, and further needs to have the capacity of adjusting pathological microenvironment, have osteoinductive activity, and can directly promote bone formation. The diabetic bone repair is a complex pathological environment and needs a process of being cooperatively realized in multiple aspects, so that an ideal treatment result cannot be achieved by a single design system. The load release of cytokines in the aspect of anti-inflammatory has the defects of high price, poor stability and the like, and the traditional antioxidant has the characteristics of easy loss and weak ROS scavenging ability. The whole system needs to have the effects of relieving inflammation pathological environment, forming bones, having corresponding mechanical support and the like, and the current clinical experimental method is limited in the aspect, so that the cooperative regeneration of the diabetic bones is difficult to achieve. Disclosure of Invention Therefore, the technical problem to be solved by the invention is to overcome the clinical problems of oxidative stress, osteogenesis dysfunction and the like of the bone repair material in the prior art in the hyperglycemic environment. In order to solve the technical problems, the invention provides the osteogenic nano enzyme doped with metal ions and the application thereof, the active metal ions are combined with the nano enzyme, and the metal elements with osteogenic activity (the substitution degree is 6-8%) are introduced by partially substituting iron ions in crystal lattices by utilizing the open crystal structure characteristics of Prussian blue nano enzyme, so that the novel bioactive function of the material can be provided on the premise of keeping the original catalytic performance of the Prussian blue nano enzyme, thereby accurately regulating the micro environment of diabetic bones and powerfully promoting the regeneration of bone tissues in a hyperglycemic environment. The osteogenic nano enzyme disclosed by the invention is integrated into the PCL bracket, so that the bracket has strong osteoinduction, antioxidation and anti-inflammatory functions, and a good foundation is laid for the application of the osteogenic nano enzyme in the regeneration of diabetic bones. The first object of the invention is to provide an osteogenic nano-enzyme doped with metal ions, which is prepared by mixing Prussian blue nano-enzyme particles with metal ions in the presence of a surfactant and a stabilizer, performing a first reaction, adding potassium ferricyanide (to enhance structural stability) after the first reaction is finished, performing a second reaction, and freeze-drying to obtain the osteogenic nano-enzyme. Further, the metal ion is selected from one or more of Mg 2+、Mn2+、Co2+、Cu2+、Zn2+、Sr2+、Mo2+ and Eu 3+. Further, the stabilizer comprises trisodium citrate. Further, the method comprises the steps of, the surfactant comprises polyvinylpyrrolidone. The second object of the invention is to provide an application of the osteogenic nano-enzyme in preparing bone repair materials. The third object of the invention is to provide an application of the osteogenic nano-enzyme in preparing a bone injury repair product caused by type 2 diabetes. The fourth object of the invention is to provide an application of the osteogenic nano-enzyme in preparing an antioxidant product. A fifth object of the present invention is to provide an osteogenic composite comprising an osteogenic nanoenzyme as described above and polycaprolactone. A sixth object of the present invention is to