CN-121987856-A - Bionic myoelectricity active hydrogel and preparation method and application thereof

Abstract

The invention provides bionic myoelectricity active hydrogel, a preparation method and application thereof, and belongs to the technical field of biological materials. The hydrogel is prepared from raw materials such as polylactic acid-silver nanowire composite material, conductive polymer material and the like. The hydrogel can generate stable voltage of 0.15V under ultrasonic stimulation through a piezoelectric-conductive synergistic mechanism, has conductivity of 1.67S/m, and has self-repairing property, tissue adhesion property and biocompatibility. In the volumetric muscle defect model, the hydrogel can obviously inhibit fibrosis, promote angiogenesis and myofiber regeneration, and realize motor function recovery. The invention provides an injectable and self-powered electroactive repair material for muscle tissue engineering.

Inventors

• YUAN DECHAO
• ZHOU YULAN
• WANG DONG
• LIU SHIWEI
• He Renjian
• LUO YUANCHAO
• YANG FUGUO

Assignees

• 自贡市第一人民医院

Dates

Publication Date

20260508

Application Date

20260213

Claims (10)

1. 1. The bionic myoelectric activity hydrogel is characterized by comprising a polylactic acid-silver nanowire composite material, a conductive polymer material and a hydrogel matrix, wherein the final concentration of the conductive polymer material in the bionic myoelectric activity hydrogel is 0.5% -2.0%, and the mass ratio of the polylactic acid-silver nanowire composite material to the conductive polymer material is (0.3% -0.8) (0.03% -0.08).
2. 2. The bionic myoelectric active hydrogel according to claim 1, wherein the final concentration of the conductive polymer material in the bionic myoelectric active hydrogel is 1%, and the mass ratio of the polylactic acid-silver nanowire composite material to the conductive polymer material is 0.5:0.05.
3. 3. The bionic myoelectric active hydrogel according to claim 1, wherein the hydrogel matrix is a product prepared from alkali, small molecules containing disulfide bonds, polyphenols and water.
4. 4. The biomimetic myoelectric active hydrogel according to claim 3, wherein the base is an inorganic base, and/or the small molecule containing disulfide is lipoic acid or a salt thereof, and/or the polyphenol compound is tannic acid; preferably, the alkali is sodium hydroxide and the small molecule containing disulfide bonds is lipoic acid.
5. 5. The bionic myoelectric active hydrogel according to claim 1, wherein the mass fraction of silver nanowires in the polylactic acid-silver nanowire composite is 0.5% -2.0%, preferably 1.0%.
6. 6. The bionic myoelectric active hydrogel according to any one of claims 1 to 5, wherein the preparation method of the polylactic acid-silver nanowire composite material comprises the steps of mixing polylactic acid and silver nanowire dispersion liquid in an organic solvent, carrying out electrostatic spinning, drying, freezing and slicing, and carrying out ultrasonic dispersion to obtain the bionic myoelectric active hydrogel; The preparation method of the conductive polymer material comprises the following steps of reacting carboxymethyl chitosan with aniline monomer and oxidant under an acidic condition, precipitating, washing, dialyzing and drying.
7. 7. A method for preparing the bionic myoelectric active hydrogel according to any one of claims 1 to 6, which is characterized by comprising the following steps of mixing and reacting a polylactic acid-silver nanowire composite material, a conductive polymer material and a raw material for preparing a hydrogel matrix.
8. 8. The method according to claim 7, wherein the reaction is carried out at a temperature of 10-40 ℃ for a time of 20-40 min.
9. 9. Use of the bionic myoelectric active hydrogel according to any one of claims 1 to 6 for preparing a muscle tissue repair material.
10. 10. The use according to claim 9, wherein the muscle tissue repair material is a volumetric muscle defect repair material.

Description

Bionic myoelectricity active hydrogel and preparation method and application thereof Technical Field The invention belongs to the technical field of biological materials, and particularly relates to bionic myoelectric activity hydrogel, and a preparation method and application thereof. Background Volumetric muscle defects (Volumetric Muscle Loss, VML) refer to skeletal muscle tissue loss beyond the self-repair capacity of the body due to high energy trauma, tumor resection, congenital malformations, or the like. Such loss of large volume muscle not only causes severe muscle strength and motor dysfunction, affecting the quality of life of the patient, but also is prone to causing muscle atrophy, chronic pain and psychological trauma. At present, clinical treatment mainly depends on autologous or allogeneic muscle transplantation, tendon translocation and tissue reconstruction operation, but faces serious challenges such as limited donor tissues, poor immune rejection, poor functional recovery, high complications and the like. Therefore, there is a need to develop new tissue engineering materials to achieve dual regeneration of structure and function. In recent years, tissue engineering has demonstrated potential in treating VML, and its core strategy is to provide structural support for cell adhesion, proliferation and differentiation by constructing three-dimensional biological scaffolds. At present, a plurality of stents are applied to VML models, including porous structural stents, injectable hydrogels, tissue templates constructed by biological printing and the like, and the volume filling of defect parts and the recovery of partial muscle functions are primarily realized. However, these materials generally emphasize biocompatibility and mechanical support, while skeletal muscle acts as a highly electroactive tissue, whose regeneration relies on precise bioelectric signal regulation, and existing materials have significant drawbacks in this regard. Electrical Stimulation (ES) has shown effect in promoting regeneration of tissues such as muscle, and research shows that it can induce stem cells to differentiate towards myogenesis by regulating cell membrane potential, activating related signal pathways. The current implementation mode of electric stimulation mainly comprises external application and in-vivo implantation, wherein the external application is dependent on special equipment and regular manual operation, an electric signal is easy to attenuate due to absorption of surrounding tissues, a battery and an electrode are required to be implanted in the in-vivo implantation, the problems of infection risk and battery toxicity exist, and the in-vivo implantation is required to be taken out through secondary operation. Therefore, the development of biomaterials having autonomous electrical signal generation capability has become an important research direction. Piezoelectric materials are used as intelligent materials capable of spontaneously generating charges under the action of external force, can respond to physiological stimulus without an external power supply, and are paid attention to in tissue engineering repair. The piezoelectric materials are mainly divided into inorganic and organic materials, wherein the inorganic materials such as barium titanate, lead zirconate titanate and the like have excellent piezoelectric properties, but have hard texture and limited biological safety, and the organic materials are represented by polyvinylidene fluoride (PVDF), poly L-lactic acid (PLLA) and the like, and have the advantages of high biological safety, good flexibility and the like, but have low piezoelectric coefficient generally. The PLLA has the defects of low piezoelectric response strength, limited charge conduction efficiency and the like, and is difficult to meet the electric signal requirement of muscle tissue regeneration. The existing research shows that the electrostatic spinning method can induce PLLA to polarized crystal through a high-voltage electric field, and is an effective means for preparing the piezoelectric PLLA material. However, the stents prepared by this method require invasive surgical implantation, may cause complications and have a long recovery period. Injectable hydrogels are ideal carriers for their advantages of non-invasive delivery and adaptation to irregular defect shapes. The PLLA nanofibers have been studied in combination with collagen hydrogels to develop degradable, injectable piezoelectric hydrogels and to apply to cartilage repair, confirming the feasibility of the PLLA and hydrogel compounding strategy. However, research on injectable PLLA composite hydrogels with both strong piezoelectric effect and high conductivity for VML repair has not been reported. In summary, the prior art has the following defects that (1) the traditional PLLA has limited piezoelectric performance and insufficient charge generation and conduction efficiency, (2) the traditional piez