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CN-121987860-A - Full-implanted muscle defect electrical stimulation biological piezoelectric system and preparation method and application thereof

CN121987860ACN 121987860 ACN121987860 ACN 121987860ACN-121987860-A

Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to a fully-implanted muscle defect electro-stimulation bio-piezoelectric system, a preparation method and application thereof, wherein the electro-stimulation bio-piezoelectric system comprises a piezoelectric nano-generator and conductive hydrogel; the piezoelectric nano generator comprises a chitosan-polyvinyl alcohol composite biological piezoelectric film, and the conductive hydrogel is obtained by ultraviolet irradiation of an aqueous solution containing methacryloyl silk fibroin, carbon nanotubes immersed in a dopamine solution and a photoinitiator. The invention has the characteristics of biocompatibility and good self-healing acceleration, and is expected to promote the development of the symbiotic electric stimulation treatment device without batteries and circuits.

Inventors

  • BAI SHUO
  • Qu Jingzong
  • SUN RUIYANG
  • LI JIELING
  • LI QI

Assignees

  • 中国科学院过程工程研究所

Dates

Publication Date
20260508
Application Date
20241105

Claims (10)

  1. 1. The fully-implanted muscle defect electro-stimulation bio-piezoelectric system is characterized by comprising a piezoelectric nano-generator and conductive hydrogel; The piezoelectric nano generator comprises a chitosan-polyvinyl alcohol composite biological piezoelectric film; The conductive hydrogel is obtained by ultraviolet irradiation of an aqueous solution containing methacryloyl silk fibroin, carbon nanotubes immersed in a dopamine solution and a photoinitiator.
  2. 2. The fully implantable muscular deficiency electrical stimulation bio-piezoelectric system according to claim 1, wherein the piezoelectric nano-generator further comprises a conductive metal electrode, a metal wire, and a polymer film; and covering conductive metal electrodes on two sides of the chitosan-polyvinyl alcohol composite biological piezoelectric film, wherein the conductive metal electrodes are connected with metal wires, and the polymer film encapsulates the chitosan-polyvinyl alcohol composite biological piezoelectric film covered with the conductive metal electrodes to obtain the piezoelectric nano generator.
  3. 3. The fully implantable muscle-defect electro-stimulation bio-piezoelectric system according to claim 2, wherein the conductive metal electrode is a zinc-based electrode, a magnesium-based electrode, a molybdenum electrode or a tungsten electrode, the metal wire is a zinc-based wire or a magnesium-based wire, and the polymer film is polylactic acid or polyhydroxyalkanoate.
  4. 4. The fully implantable electrical stimulation bio-piezoelectric system for muscle defects according to claim 1, wherein the mass ratio of chitosan to polyvinyl alcohol in the chitosan-polyvinyl alcohol composite bio-piezoelectric film is 1:1 or more.
  5. 5. The fully implantable electrical stimulation bio-piezoelectric system according to claim 1, wherein the thickness of the chitosan-polyvinyl alcohol composite bio-piezoelectric film is 10-100 microns.
  6. 6. A method of preparing a fully implantable electrical stimulation bio-piezoelectric system for muscle defects, the method comprising: preparation of a piezoelectric nano generator: Covering the upper and lower surfaces of the chitosan-polyvinyl alcohol composite biological piezoelectric film with biodegradable conductive metal electrodes as electrodes, extending the electrodes by using biodegradable metal wires as wires, and packaging the electrodes by using a biodegradable and bio-friendly polymer film to obtain the piezoelectric nano generator; Preparation of conductive hydrogel: Dissolving the methacryloyl silk fibroin in water, adding a photoinitiator, mixing until the solution is completely dissolved, simultaneously cleaning the carbon nano tube immersed in the dopamine solution by using ultrapure water, adding the cleaned carbon nano tube into the solution to obtain the carbon nano tube-methacryloyl silk fibroin solution, wherein the content of the carbon nano tube is 1-10mg/mL, and then irradiating the carbon nano tube-methacryloyl silk fibroin conductive hydrogel by using ultraviolet rays.
  7. 7. The method of preparing the chitosan-polyvinyl alcohol composite bio-piezoelectric film according to claim 6, wherein the preparation of the chitosan-polyvinyl alcohol composite bio-piezoelectric film comprises: Mixing chitosan solution with concentration of 0.5-2w/v% with polyvinyl alcohol solution with concentration of 4-9w/v%, and drying to obtain the chitosan-polyvinyl alcohol composite biological piezoelectric film at 30-40 ℃.
  8. 8. The method according to claim 7, wherein the mixing time of the chitosan solution and the polyvinyl alcohol solution is not less than 8 hours.
  9. 9. The method according to claim 6, wherein the concentration of the methacryloyl silk fibroin in the carbon nanotube-methacryloyl silk fibroin solution is 8-20w/v%, and the concentration of the photoinitiator is 0.1-0.5w/v%.
  10. 10. Use of the fully implantable electrical muscle-defect biostimulation piezoelectric system according to any one of claims 1-5 or the fully implantable electrical muscle-defect biostimulation piezoelectric system prepared by the preparation method according to any one of claims 6-9 for preparing a biomaterial for muscle repair.

Description

Full-implanted muscle defect electrical stimulation biological piezoelectric system and preparation method and application thereof Technical Field The invention relates to the technical field of biomedical materials, in particular to a full-implanted muscle defect electro-stimulation bio-piezoelectric system consisting of a composite bio-piezoelectric film and conductive hydrogel, and a preparation method and application thereof. Background Physiological electrical stimulation is critical to maintaining balance within the tissue. Significant medical success has been achieved with external electrical stimulation to repair tissue damage. However, some of the electrical stimulation devices currently on the market present a potential risk of damaging human health. In prior art approaches, it is often necessary to implant the electrically conductive device by invasive surgery, which involves a significant risk of infection by way of electrical stimulation via wires. In addition, when it is desired to remove the electrically conductive device, a secondary operation is often also required, which undoubtedly increases the physical burden and economic cost of the patient. To reduce these risks, future research may be focused on developing safer, more effective electrical stimulation devices, and on finding electrical conduction methods that are less susceptible to infection. Currently, the prior art is directed to the risk of infection that electrical stimulation devices may cause during implantation and removal, and how to effectively avoid these problems, and lacks a complete solution. Disclosure of Invention Aiming at the defects in the prior art, the invention provides a full-implanted muscle defect electro-stimulation (MD-ES) biological piezoelectric system, and the obtained full-implanted muscle defect electro-stimulation biological piezoelectric system has good biocompatibility, can reduce the infection risk generated by the full-implanted muscle defect electro-stimulation biological piezoelectric system compared with the existing electro-stimulation device, and has better muscle tissue growth inducibility. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: In a first aspect, the invention provides a composite bio-piezoelectric thin film device, specifically, a composite bio-piezoelectric thin film is obtained by uniformly mixing chitosan solution and polyvinyl alcohol solution, adding the mixture into a culture dish on a heating table, and drying. And covering the upper and lower surfaces of the composite bio-piezoelectric film with biodegradable metal electrodes as electrodes, extending the biodegradable metal wires as wires, and packaging with biodegradable and bio-friendly polymer films to obtain the composite bio-piezoelectric film device, wherein the composite bio-piezoelectric film device is used as a piezoelectric nano-generator in an electric stimulation bio-piezoelectric system. The composite biological piezoelectric film device can generate output voltage after being acted by force, can generate 0.2V output voltage at the highest under the condition of pressure stimulation, has the conductivity of 0.06S/m at the highest, and can electrically stimulate organism tissues by the generated electric signals, thereby promoting the repair of muscle injury. In a second aspect, the present invention provides a method for preparing the composite bio-piezoelectric thin film device according to the first aspect, which specifically includes the following steps: Step 1, preparation of chitosan solution: 1g of chitosan (average molecular weight 890000,Sigma Aldrich) and glacial acetic acid (the amount of glacial acetic acid is 1-1.5mL per hundred mL) are mixed in 100mL of aqueous solution to obtain 1w/v% chitosan solution, and the concentration of the chitosan solution is 0.5-2 w/v%. Step 2, preparing a polyvinyl alcohol solution: 5g of polyvinyl alcohol (PVA) (viscosity average molecular weight Mv 13000-23000,Sigma Aldrich) was dissolved in 100mL of deionized water, and an aqueous polyvinyl alcohol solution having a concentration of 5w/v% and an aqueous polyvinyl alcohol solution having a concentration of 4-9 w/v% was prepared. To ensure uniformity of the solution, the mixture was stirred at room temperature for 48 hours or more to obtain a polyvinyl alcohol solution. Step 3, preparing a chitosan-polyvinyl alcohol mixed solution: and adding a chitosan solution with a certain volume capacity of 1w/v% into a PVA solution with a certain volume capacity of 5w/v% to obtain a chitosan-PVA solution mixed according to different actual conditions. Stirring was continued with a magnetic stirrer at 30-40℃for 6-8 hours to give a homogeneous solution of chitosan and PVA. Step 4, preparing a chitosan-polyvinyl alcohol composite biological piezoelectric film: At room temperature, a homogeneous solution of chitosan and PVA was slowly poured into a petri dish. The low surface tensi