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CN-121988064-A - Device and process for extracting crop high-temperature stress resistant substances by using ultrasonic waves

CN121988064ACN 121988064 ACN121988064 ACN 121988064ACN-121988064-A

Abstract

The invention is suitable for the technical field of extraction of high-temperature stress resistant substances of crops, and provides a device and a process for extracting the high-temperature stress resistant substances of the crops by ultrasonic waves. Compared with the traditional extraction method, the method has the advantages that the extraction time is shorter, the extraction rate is higher, the used device is compact in structure and simple and convenient to operate, the operation technical requirements are reduced, large-scale application is possible, a large amount of organic solvents are not needed in the extraction process, the environmental pollution is reduced, the activity of the extracted substances can be better kept, and the generation power of an ultrasonic energy gathering rod is gradually increased, so that the extraction process is milder, and the quality of the extracted substances is further ensured.

Inventors

  • LI JIE
  • HAO ERBAO
  • LI YANG

Assignees

  • 捷玛(青岛)生物科技有限公司

Dates

Publication Date
20260508
Application Date
20251215

Claims (10)

  1. 1. The device for ultrasonically extracting the high-temperature stress resistant substances of the crops is characterized by comprising an ultrafine grinder (1), a shearing and dispersing stirring integrated reaction kettle (2), a low-speed stirring storage tank (3), a gear pump (4), a reactor component and an emulsifying and stirring reaction kettle (9); The superfine pulverizer (1), the shearing and dispersing stirring integrated reaction kettle (2), the low-speed stirring storage tank (3) and the gear pump (4) are connected through pipelines in sequence; The gear pump (4) is communicated with the reactor component, a speed-control liquid channel (10) is arranged between the reactor component and the emulsifying and stirring reaction kettle (9), and a fine filtering component (11) is communicated between the reactor component and the emulsifying and stirring reaction kettle through a pipeline.
  2. 2. The device for ultrasonically extracting high temperature stress resistant substances from crops according to claim 1, wherein the reactor assembly comprises a first reactor (6), a second reactor (7) and a third reactor (8), and the first reactor (6), the second reactor (7) and the third reactor (8) are sequentially connected in series.
  3. 3. The device for ultrasonically extracting high temperature stress resistant substances from crops according to claim 2, wherein the speed control liquid channel (10) is connected with a servo valve (5).
  4. 4. A device for ultrasonically extracting substances against high temperature stress from crops as claimed in claim 3, wherein the speed control liquid channel (10) between the first reactor (6) and the servo valve (5) is connected with a flow rate signal detector (12), and the flow rate signal detector (12) is connected with the gear pump (4) and the servo valve (5).
  5. 5. The device for ultrasonically extracting high temperature stress resistant substances from crops according to claim 4, wherein the tops of the first reactor (6), the second reactor (7) and the third reactor (8) are respectively provided with an ultrasonic energy gathering rod (13).
  6. 6. The device for ultrasonically extracting high temperature stress resistant material of crops as claimed in claim 5, wherein the fine filter assembly (11) is a centrifugal filter.
  7. 7. A process for extracting a crop high temperature stress resistant material using the device of any one of claims 1 to 6, comprising the steps of: S1, sun-drying crops containing high-temperature stress resistant substances; S2, crushing the dried crops to 200 meshes by using an ultrafine crusher (1); s3, adding water into crushed crops according to a proportion, and placing the crushed crops into a shearing, dispersing and stirring integrated reaction kettle (2) to perform continuous constant-temperature dispersing and stirring; S4, transferring the flowing mixture into a low-speed stirring storage tank (3), circulating the mixture through a speed control liquid channel (10) from the rotating speed of a gear pump (4), controlling the flow speed during circulation, enabling the mixture to sequentially flow through a first reactor (6), a second reactor (7) and a third reactor (8), starting an ultrasonic energy collecting rod (13), and gradually increasing the generation power of the ultrasonic energy collecting rod (13) according to the flowing sequence of the mixture; S5, performing solid-liquid analysis on the mixture flowing out of the pipeline by adopting a centrifugal filter, and reserving clear liquid for standby; s6, placing the standby clear liquid into an emulsification and stirring type reaction kettle (9), adding the compound amino acid powder, continuously emulsifying and stirring at normal temperature for 30min to obtain a temperature-resistant product, discharging the mixed liquid, sealing and storing the mixed liquid in a shady and cool dry place.
  8. 8. The process according to claim 7, wherein the water content of the crop containing the high temperature stress resistant material in S1 is less than 5%, and the crop is rinsed with clean water prior to sun drying.
  9. 9. The process according to claim 7, wherein the rotation speed of the shearing, dispersing and stirring integrated reaction kettle (2) in the step S3 is set to 900r/min, the stirring rotation speed is set to 50r/min, the stirring is required to have a wall scraping function, the dispersing duration is 30min, and the stirring time is continued until all the mixture flows; The mass ratio of the crops to the water in the S3 is 1:10-1:12; The temperature in the step S3 is controlled to be 30-40 ℃.
  10. 10. The process according to claim 7, wherein the pipeline flow rate in S4 is set to be 6-10 m/min, the generation power of the ultrasonic energy gathering rod (13) is set to be 75-100 kHz, the rotating speed of the centrifugal filter in S5 is set to be 1800-2000r/min, and the centrifugal quantity is set to be 0.8-1t/h.

Description

Device and process for extracting crop high-temperature stress resistant substances by using ultrasonic waves Technical Field The invention relates to the technical field of extraction of high-temperature stress resistant substances of crops, in particular to a device and a process for extracting the high-temperature stress resistant substances of crops by ultrasonic waves. Background Crop high temperature stress resistant substances include, but are not limited to, heat shock proteins in plants, antioxidants, osmoregulating substances, and the like. Crops are easily affected by heat stress in a high-temperature environment, so that the growth and development are inhibited, even serious physiological barriers are generated, and the yield and quality of the crops are affected. By extracting and researching the key substances of the crops for resisting high temperature, the reaction mechanism of the crops for resisting high temperature can be deeply known, so that a new variety for resisting high temperature is developed or crops with higher heat resistance are bred, and the influence of heat stress on agricultural production is reduced. Therefore, the research on the substances of crops for resisting high temperature stress and the extraction method thereof have important significance for improving the stress resistance of crops and ensuring agricultural production. In the prior art, the extraction method of the crop high temperature stress resistant substances is various, and comprises a solvent extraction method, a microwave-assisted extraction method, a supercritical fluid extraction method and the like. However, these methods often have problems such as low extraction efficiency, complicated operation, high cost, and the like. For example, the solvent extraction method needs to use a large amount of organic solvents, which not only causes environmental pollution, but also easily causes the reduction of the activity of substances in the extraction process, the microwave-assisted extraction method has high equipment cost and high operation technical requirements although the extraction speed is high, and the supercritical fluid extraction method needs high-pressure equipment, has harsh operation conditions and is difficult to apply on a large scale. Therefore, it is important to develop a high-efficiency, environment-friendly and low-cost extraction method for crop high-temperature stress resistant substances. Disclosure of Invention The invention aims to provide a device and a process for extracting crop high-temperature stress resistant substances by ultrasonic waves, so as to solve the problems in the background technology. In order to achieve the aim, the invention provides the technical scheme that the first aspect of the invention provides a device for extracting crop high temperature stress resistant substances by ultrasonic waves, which comprises an ultrafine grinder, a shearing and dispersing stirring integrated reaction kettle, a low-speed stirring storage tank, a gear pump, a reactor component and an emulsifying and stirring reaction kettle; the superfine pulverizer, the shearing and dispersing stirring integrated reaction kettle, the low-speed stirring storage tank and the gear pump are connected through pipelines in sequence; the gear pump is communicated with the reactor component, a speed control liquid channel is arranged between the gear pump and the reactor component, and a fine filtering component is communicated between the reactor component and the emulsifying and stirring reaction kettle through a pipeline. Preferably, the reactor assembly comprises a first reactor, a second reactor and a third reactor, and the first reactor, the second reactor and the third reactor are sequentially connected in series. Preferably, the speed control liquid channel is connected with a servo valve. Preferably, a speed control liquid channel between the first reactor and the servo valve is connected with a flow speed signal detector, and the flow speed signal detector is connected with the gear pump and the servo valve. Preferably, the tops of the first reactor, the second reactor and the third reactor are respectively provided with an ultrasonic energy gathering rod in a penetrating way. Preferably, the fine filter assembly is a centrifugal filter. The second aspect of the invention provides a process for extracting high temperature stress resistant substances from crops by using the device of the first aspect of the invention, which is characterized by comprising the following steps: S1, sun-drying crops containing high-temperature stress resistant substances; s2, crushing the dried crops to 200 meshes by using an ultrafine crusher; S3, adding water into crushed crops according to a proportion, and placing the crushed crops into a reaction kettle integrating shearing, dispersing and stirring into a whole, and continuously dispersing and stirring at a constant temperature; S4, transferring the flowing m