CN-121988065-A - Preparation method of embryosin at peanut stem end meristem

CN121988065ACN 121988065 ACN121988065 ACN 121988065ACN-121988065-A

Abstract

The invention relates to the technical field of embryon production, in particular to a production method of embryon at a stem end meristem of peanut, which comprises the steps of S1, obtaining a target segment of the stem end meristem of peanut embryo in a preset growth period, S2, cleaning and crushing the target segment, mixing the target segment with entrainer in an extraction kettle according to a preset proportion, S3, introducing carbon dioxide at a preset temperature and a preset pressure into the extraction kettle, completing extraction in the preset period, S4, sequentially introducing the extracted carbon dioxide into a primary separator and a secondary separator, separating the embryon, S5, respectively obtaining a pre-separation characteristic value and a post-separation characteristic value, S6, primarily judging whether the extraction of embryon meets the preset standard, determining the reason that the extraction of embryon does not meet the preset standard, S7, primarily judging whether the extraction of embryon meets the preset standard, and determining the cyclic extraction of the secondary separator based on the post-separation characteristic value. The invention improves the quality of the product and stabilizes the extraction efficiency.

Inventors

LI YUANHAO
XIE PEIZENG
LI MEIHUA
DENG YIN

Assignees

广州新征程生物科技有限公司
广东新征程生命科学研究院

Dates

Publication Date: 20260508
Application Date: 20251218

Claims (10)

1. The preparation method of embryosin at the meristem of peanut stem end is characterized by comprising the following steps: step S1, obtaining a target segment of a stem end meristem on peanut embryo in a preset growing period; S2, cleaning the target section, crushing to obtain a base material, and mixing the base material and an entrainer in an extraction kettle according to a preset proportion to obtain a pretreatment material; Step S3, introducing carbon dioxide at a preset temperature and a preset pressure into the extraction kettle, starting an ultrasonic generator and completing extraction in a preset period of time; S4, sequentially introducing the extracted carbon dioxide into a primary separator and a secondary separator to separate embryoid bodies, wherein the separation temperature and the separation pressure of the primary separator and the secondary separator are different; S5, obtaining cooling flow of the first-stage separator and the second-stage separator, and respectively obtaining a front separation characteristic value and a rear separation characteristic value according to the cooling flow of each stage separator and the weight of a target section; Step S6, when the extraction of the embryo element is preliminarily judged to be not in accordance with a preset standard based on the pre-separation characteristic value, determining the reason for the failure to be in accordance with the preset standard according to the purity of the embryo element separated by the primary separator, and verifying that the extraction of the embryo element is in accordance with the preset standard according to the polypeptide content of the embryo element; and S7, determining whether the secondary separator circularly extracts or not based on the post-separation characteristic value when the pre-separation characteristic value initially judges that the extraction of embryo elements meets the preset standard.
2. The method for producing embryon at a meristem of peanut stem according to claim 1, wherein the preliminary determination that embryon extraction does not meet a preset criterion is made in response to the pre-separation characteristic value being less than a second preset pre-separation characteristic threshold, and the preliminary determination that embryon extraction meets a preset criterion is made in response to the pre-separation characteristic value being greater than or equal to the second preset pre-separation characteristic threshold; the pre-separation characteristic value is determined jointly based on the cooling flow of the primary separator, the target segment weight, and a separation characteristic threshold.
3. The method for producing embryon at a meristem of peanut stem according to claim 2, wherein the reason for not meeting a preset standard is determined according to the embryon purity separated by the primary separator in response to the pre-separation characteristic value being smaller than a first preset pre-separation characteristic threshold, and the extraction of embryon according to the embryon polypeptide content is verified to meet a preset standard in response to the pre-separation characteristic value being greater than or equal to the first preset pre-separation characteristic threshold and smaller than a second preset pre-separation characteristic threshold.
4. The method for preparing embryosin at a meristem of peanut according to claim 3, wherein the extraction of embryosin is verified to meet a predetermined criterion based on the content of embryosin polypeptide, If the content of the embryo extract polypeptide is smaller than a preset activity threshold, verifying that the embryo extract does not meet a preset standard, and synchronously reducing the separation temperature of the primary separator and the secondary separator according to the reduction of the difference between the preset activity threshold and the embryo extract polypeptide content; if the content of the embryo extract polypeptide is larger than or equal to a preset activity threshold, the embryo extract is verified to meet a preset standard.
5. The method of claim 4, wherein the magnitude of the simultaneous decrease in separation temperature of the primary separator and the secondary separator is directly related to the difference between the predetermined activity threshold and the polypeptide content of embryonin.
6. The method for producing embryodins at a meristem of peanut according to claim 5, wherein the separation temperatures of the primary separator and the secondary separator are synchronously corrected according to a difference between a preset extraction efficiency and an extraction efficiency of embryodins under a first preset condition; The first preset condition is that the primary separator and the secondary separator complete synchronous reduction of the separation temperature, and the extraction efficiency of the embryoid bodies after the reduction of the separation temperature is smaller than the preset extraction efficiency.
7. The method of claim 6, wherein the reason for the failure to meet the preset standard is determined based on the purity of the embryon isolated by the primary separator, If the embryo element purity is smaller than a preset purity threshold, determining that the reason that the embryo element purity does not meet the preset standard is that the separation temperature does not reach the standard; If the embryo element purity is greater than or equal to a preset purity threshold, determining that the reason for not meeting the preset standard is that the separation pressure is not up to standard.
8. The method of claim 7, wherein in response to the separation temperature not reaching the standard, reducing the separation temperature based on a difference between the embryo's purity and a predetermined purity threshold; Wherein the separation temperature adjustment amplitude is positively related to the difference between the embryo element purity and a preset purity threshold; Responding to the separation pressure not reaching the standard, and improving the separation pressure according to the difference value between the embryo element purity and a preset purity threshold; Wherein the separation pressure adjustment magnitude is positively related to the difference between the embryo element purity and a preset purity threshold.
9. The method for producing embryodins at a meristem of peanut stem of claim 8, wherein determining whether the secondary separator is circularly extracted based on the post-separation characteristic value under a second preset condition; If the post-separation characteristic value is smaller than a first preset post-separation characteristic threshold value, introducing the carbon dioxide in the secondary separator into the primary separator, and separating again; If the post-separation characteristic value is greater than or equal to a first preset post-separation characteristic threshold value, introducing the carbon dioxide in the secondary separator into a recovery device; wherein the second preset condition is that the extraction of the embryo element meets a preset standard.
10. The method for producing embryodins at a meristem of peanut stem according to claim 9, wherein a separation pressure of the primary separator is higher than a separation pressure of the secondary separator, and a separation temperature of the primary separator is higher than a separation temperature of the secondary separator.

Description

Preparation method of embryosin at peanut stem end meristem Technical Field The invention relates to the technical field of embryosin preparation, in particular to a preparation method of embryosin at a peanut stem end meristem. Background When extracting embryon at the stem end meristem of peanut by using the traditional organic solvent extraction method, the problems of solvent residue, inactivation of active ingredients caused by high temperature, more impurities in the extract, complicated subsequent purification steps and the like exist; The parameters of the conventional supercritical extraction process are usually preset and fixed, but in actual production, batch differences exist in raw materials, such as growth state, water content and the like, the fixed parameters cannot ensure that the quality and extraction efficiency of each batch of products reach the standard stably, and after extraction, available active ingredients may still be contained in carbon dioxide, so that direct discharge can cause waste. CN118750903a discloses a method for extracting black garlic stock solution by supercritical carbon dioxide, which comprises pretreating black garlic, extracting supercritical carbon dioxide by using entrainer and surfactant, and reasonably designing parameters of supercritical carbon dioxide extraction and types and dosage of entrainer and surfactant, thereby effectively improving extraction rate of effective components such as alliin, deoxyalliin, etc. Therefore, the invention uses the low-toxicity compound dodecyl sulfate as the surfactant, has potential safety hazard, can not adjust the process parameters in real time, and has the problems of unstable product quality and extraction efficiency. Disclosure of Invention Therefore, the invention provides a preparation method of embryosin at the meristem of peanut stem, which is used for solving the problems of unstable quality and extraction efficiency of products in the prior art. In order to achieve the above purpose, the invention provides a method for preparing embryo element at peanut stem end meristem, comprising the following steps: step S1, obtaining a target segment of a stem end meristem on peanut embryo in a preset growing period; S2, cleaning the target section, crushing to obtain a base material, and mixing the base material and an entrainer in an extraction kettle according to a preset proportion to obtain a pretreatment material; Step S3, introducing carbon dioxide at a preset temperature and a preset pressure into the extraction kettle, starting an ultrasonic generator and completing extraction in a preset period of time; S4, sequentially introducing the extracted carbon dioxide into a primary separator and a secondary separator to separate embryoid bodies, wherein the separation temperature and the separation pressure of the primary separator and the secondary separator are different; S5, obtaining cooling flow of the first-stage separator and the second-stage separator, and respectively obtaining a front separation characteristic value and a rear separation characteristic value according to the cooling flow of each stage separator and the weight of a target section; Step S6, when the extraction of the embryo element is preliminarily judged to be not in accordance with a preset standard based on the pre-separation characteristic value, determining the reason for the failure to be in accordance with the preset standard according to the purity of the embryo element separated by the primary separator, and verifying that the extraction of the embryo element is in accordance with the preset standard according to the polypeptide content of the embryo element; and S7, determining whether the secondary separator circularly extracts or not based on the post-separation characteristic value when the pre-separation characteristic value initially judges that the extraction of embryo elements meets the preset standard. Further, initially determining that the extraction of embryon does not meet a preset criterion in response to the pre-separation characteristic value being less than a second preset pre-separation characteristic threshold, and initially determining that the extraction of embryon meets a preset criterion in response to the pre-separation characteristic value being greater than or equal to the second preset pre-separation characteristic threshold; the pre-separation characteristic value is determined jointly based on the cooling flow of the primary separator, the target segment weight, and a separation characteristic threshold. Further, determining a cause of the failure to meet a preset standard according to the embryo extract purity separated by the primary separator in response to the pre-separation characteristic value being less than a first preset pre-separation characteristic threshold, and verifying that the extraction of embryo extract meets the preset standard according to the embryo extract polypeptide content in respons