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CN-121988068-A - Method for simultaneously extracting forsythoside A and xylooligosaccharide by treating forsythia leaves through steam explosion technology and application of method

CN121988068ACN 121988068 ACN121988068 ACN 121988068ACN-121988068-A

Abstract

The invention discloses a method for simultaneously extracting forsythoside A and xylooligosaccharide from forsythia leaves by adopting a steam explosion technology and application thereof, comprising the following steps: pre-soaking weeping forsythia leaf fragments, putting the pre-soaked weeping forsythia leaf fragments into a steam explosion storage tank for explosion, and respectively extracting by using an alcohol extraction method and an acidolysis method after treatment to obtain a coarse extract of forsythoside A and xylooligosaccharide. The invention utilizes the steam explosion technology to treat the weeping forsythia leaves, generates strong shearing force through instant pressure release of high-temperature and high-pressure steam, effectively damages the crystal structure of lignocellulose, promotes the breaking of glycosidic bonds of hemicellulose main chains, reduces the polymerization degree of xylan, generates the loose porous structure formed by explosion and increases the surface area at the same time of xylooligosaccharide, and promotes the dissolution of forsythoside A. The forsythia leaves picked in the eighth month of the blasting can obtain the forsythiaside A33.42 mg/g, and meanwhile, the xylo-oligosaccharide is obtained by about 33.3 g/kg, and compared with the raw materials which are not blasted, the content of the forsythiaside A is improved by about 14.61%.

Inventors

  • XIE HUI
  • SONG ANDONG
  • LI JINGYING
  • JIANG HANTING
  • DING WENTAO
  • ZHANG HONGSEN
  • MAO GUOTAO
  • SU ZENGPING

Assignees

  • 河南农业大学

Dates

Publication Date
20260508
Application Date
20260122

Claims (10)

  1. 1. A method for simultaneously extracting forsythoside A and xylooligosaccharide from forsythia leaves by utilizing a steam explosion technology is characterized by comprising the following steps: (1) Crushing weeping forsythia leaves, presoaking, then putting the crushed weeping forsythia leaves into a steam explosion storage tank for steam explosion, collecting materials, and drying to obtain an explosion weeping forsythia leaf material A containing forsythiaside A and xylooligosaccharide; (2) Extracting forsythoside A from the blasted forsythia suspensa leaf material A by adopting an alcohol extraction method, and extracting xylooligosaccharide from the blasted forsythia suspensa leaf material A by adopting an acidolysis method.
  2. 2. The method of claim 1, wherein in the step (1), the solvent used for the pre-soaking is (1-5) wt% of aqueous solution of citric acid, the mass ratio of solid to liquid during the pre-soaking is 1 (0.5-0.7), and the pre-soaking time is 10-15 h.
  3. 3. The method according to claim 1, wherein in the step (1), the pressure of the steam explosion is 0.6 to 1.6 MPa and the dwell time is 30 to 300 s.
  4. 4. The method of claim 1, wherein in step (1), the temperature of the baked goods is no more than 60 ℃.
  5. 5. The method of claim 1, wherein in the step (2), the solvent used for the alcohol extraction is 60-90 v% methanol, the solid-liquid mass ratio is1 (10-20), the ultrasonic treatment is carried out for 20-60 min under the condition of 30~50W,200~300 kHz, and the leaching solution B is obtained by filtering with filter paper.
  6. 6. The method according to claim 1, wherein the process of extracting the xylo-oligosaccharide by acidolysis comprises the following steps: 1-2 g of blasted forsythia suspensa leaf material A is weighed and dissolved in a sulfuric acid solution filled with 80~150 mL 0.005~0.01 mol/L, after ultrasonic treatment is carried out for 5-20 min, solid-liquid separation is carried out by using medium-speed qualitative filter paper, a filtrate is taken, and a nylon membrane with the water content of 0.22 mu m is used for filtering to obtain a sample solution before hydrolysis for later use; And (3) preparing a sample after hydrolysis, namely sucking a sample solution before hydrolysis into a colorimetric tube, adding 3-6 mol/L sulfuric acid solution, shaking uniformly, placing into a boiling water bath for hydrolysis for 80-120 min, taking out the sample, cooling, fixing the volume to a scale, shaking uniformly, sampling, and filtering with a water-based 0.22 mu m nylon membrane to obtain the sample.
  7. 7. The method according to claim 6, wherein the volume ratio of the sample solution before hydrolysis and the sulfuric acid solution is (8-10): 1, and the volume of the sample solution before hydrolysis is 5-20 mL.
  8. 8. The use of forsythoside a and xylo-oligosaccharide prepared by the method according to any one of claims 1 to 4 as livestock feed additives, wherein the forsythoside a and xylo-oligosaccharide refer to a blasted forsythoside a leaf material a containing forsythoside a and xylo-oligosaccharide obtained in the step (1).
  9. 9. The use according to claim 8, wherein the amount of the blasted forsythia suspensa leaf material a is 0.025% -0.075% of the base material mass.
  10. 10. The use according to claim 8, wherein the livestock and poultry are cattle, pigs or chickens.

Description

Method for simultaneously extracting forsythoside A and xylooligosaccharide by treating forsythia leaves through steam explosion technology and application of method Technical Field The invention relates to the field of recycling of waste forsythia leaves, in particular to a method for simultaneously extracting forsythoside A and xylooligosaccharide from forsythia leaves by adopting a steam explosion technology and application thereof. Background The annual forsythia fruit is planted in the province of China, the yield of the produced stem leaves is more than 70,000 tons, but the existing forsythia fruit leaves are not systematically utilized as non-medicinal parts, and the research shows that the forsythia fruit leaves have the same active ingredients as those in fruits, and the content of part of key ingredients is even obviously higher than that of the fruits, for example, the content of the forsythin in the forsythia fruit leaves is up to 1.5%, the content of the forsythin in the forsythia fruit leaves is 4.7 times, the content of the forsythin in the forsythia fruit stems is6 times, and the content of the forsythia fruit branches is 5.6 times. The highest content of forsythoside A in different parts is also forsythia leaf, the content is 4.5 percent, 10.6 times of branch stalk and 2.4 times of fruit stalk, and the active components have good anti-inflammatory, antioxidant and even liver protecting effects, and when the forsythoside A is used as a feed additive, the forsythoside A can also resist bacteria and viruses, improve intestinal flora, enhance immunity, reduce stress of livestock and poultry, improve production performance and the like. Meanwhile, lignocellulose which is rich in weeping forsythia leaves can be degraded and converted into xylooligosaccharide, namely a prebiotic with the characteristic of super-strong bifidus factor, so that the animal intestinal flora can be effectively regulated, the immune barrier function can be enhanced, and the antibiotic dependence can be reduced. Therefore, if the traditional Chinese medicine active ingredients and xylo-oligosaccharide in the weeping forsythia leaves can be synchronously released, a green compound feed additive with biological activity and probiotic functions is expected to be constructed, and a new technical path is provided for 'antibiotic-free cultivation'. At present, the extraction of the active ingredients of the forsythia leaves is mainly carried out by methods such as organic solvent extraction, soxhlet reflux, microwave-assisted extraction and the like, and the technology is mature, but the problems of high solvent consumption, high energy consumption, long extraction period, high equipment cost and the like generally exist. More importantly, lignocellulose in the weeping forsythia leaves has compact structure, and the degradation resistance of the cell walls seriously hinders the dissolution of active ingredients and the conversion and utilization of cellulose. Therefore, an efficient pretreatment technology is required to be introduced to destroy the cell wall structure of the plant, synchronously improve the extraction rate of active ingredients and promote the conversion of hemicellulose into xylooligosaccharide. The instantaneous ejection type steam explosion technology is used as a physical and chemical synergistic pretreatment means, and shear force is generated by instantaneous pressure release of high-temperature and high-pressure steam, so that the crystal structure of lignocellulose can be effectively destroyed, the specific surface area is increased, and favorable conditions are created for subsequent component release. The application aims to pretreat the forsythia leaves by adopting an instantaneous ejection type steam explosion technology and aims to realize the double aims of destroying a cell wall structure, promoting the dissolution of active ingredients such as forsythoside A and the like, degrading hemicellulose into xylooligosaccharide and realizing the efficient conversion of lignocellulose resources. By optimizing parameters such as steam pressure, maintenance time, pretreatment reagent and the like, an integrated treatment process capable of synchronously extracting active ingredients and generating xylooligosaccharide is constructed, and finally, the composite feed additive with the functions of Chinese medicine activity and prebiotics is created. Disclosure of Invention The invention aims to provide a method for treating weeping forsythia leaves by adopting a steam explosion technology and simultaneously extracting forsythoside A and xylooligosaccharide and application thereof, wherein the method is used for carrying out explosion pretreatment on waste non-medicinal parts of weeping forsythia She Zhengqi, destroying lignocellulose structure and degrading hemicellulose to obtain xylooligosaccharide, and simultaneously promoting the dissolution of an active ingredient forsythoside A and improving the utiliz