CN-121988211-A - Double emulsion detection feedback regulation and control system and method

CN121988211ACN 121988211 ACN121988211 ACN 121988211ACN-121988211-A

Abstract

The invention discloses a double emulsion detection feedback regulation and control system and a method, and belongs to the technical field of microfluidics. The system comprises a liquid storage device, a pressure pumping device, a microfluidic chip, a double emulsion collecting tank, an image acquisition device and a data processing terminal. The microfluidic chip comprises two serially connected micro-step structures, and the static instability of the Rayleigh-Prague is utilized to realize the staged breaking and generation of double emulsion. The image acquisition device acquires double emulsion images at the outlet of the chip in real time, data are transmitted to a data processing terminal with a built-in trained mask_ RCNN model, parameters of double emulsion microdroplets are identified and counted, the pressure pumping device is controlled in a feedback mode according to the identification result, self-adaptive adjustment of pressure is achieved, and uniform and stable particle size of the double emulsion is ensured. The invention solves the problems of dependence on manual regulation, uneven particle size and the like of the existing method, and realizes the automatic and stable preparation of double emulsion.

Inventors

WEI CHUNYANG
HUANG AO
DING YANJING
LI WANQI
LI QINGHUA
CHEN LEI

Assignees

山东第一医科大学(山东省医学科学院)

Dates

Publication Date: 20260508
Application Date: 20260408

Claims (10)

1. A double emulsion detection feedback regulation system, comprising: the liquid storage device is used for storing disperse phase fluid, continuous phase fluid and intermediate phase fluid; The pressure pumping device is communicated with the liquid storage device; the microfluidic chip is communicated with the liquid storage device and comprises a first ladder emulsifying structure and a second ladder emulsifying structure which are connected in series; The double emulsion collecting tank is communicated with the microfluidic chip; the image acquisition device acquires a double emulsion image generated at the outlet of the microfluidic chip; And the data processing terminal is internally provided with a trained visual identification model, and is used for carrying out example segmentation on the double emulsion image acquired by the image acquisition device, identifying and counting parameters of double emulsion microdroplets, and controlling the air pressure output value of the pressure pumping device according to the identification result.
2. The double emulsion detection feedback regulation system of claim 1 wherein the pressure pumping device comprises a pressure pump and a pressure controller, the pressure pump in communication with the pressure controller, the pressure controller in communication with the reservoir device.
3. The double emulsion detection feedback regulation system of claim 1, wherein the liquid storage device comprises three liquid storage containers, in particular a dispersed phase liquid storage bottle for storing dispersed phase fluid, an intermediate phase liquid storage bottle for storing intermediate phase fluid and a continuous phase liquid storage bottle for storing continuous phase fluid.
4. The double emulsion detection feedback regulation system of claim 3, wherein the dispersed phase liquid storage bottle, the intermediate phase liquid storage bottle and the continuous phase liquid storage bottle are connected with a first step emulsion structure in the microfluidic chip through fluid pipelines.
5. The double emulsion detection feedback regulation system of claim 1 wherein the trained visual recognition model employs a mask_ RCNN model.
6. The double emulsion detection feedback regulation system of claim 1, wherein the parameters for identifying and counting double emulsion droplets are specifically identifying the boundary between the inner core and the outer shell of the double emulsion droplets, and calculating the inner diameter, the outer diameter, the area of the single inner core and the area of the double droplets of the double emulsion.
7. The double emulsion detection feedback regulation system of claim 6 wherein the control of the pressure pumping device based on the identification results is specifically: The data processing terminal compares the real-time state of the double emulsion microdroplet with the state of the target double emulsion microdroplet, outputs pressure adjustment parameters according to the result and feeds the pressure adjustment parameters back to the pressure pumping device in real time, so that the self-adaptive equalization of the inner diameter of the double emulsion and the outer diameter of the spherical shell is controlled.
8. A double emulsion detection feedback regulation method applied to the double emulsion detection feedback regulation system of any one of claims 1 to 7, comprising: Starting a pressure pumping device to drive disperse phase fluid, intermediate phase fluid and continuous phase fluid in a liquid storage device to enter a microfluidic chip, wherein the microfluidic chip comprises a first step emulsifying structure and a second step emulsifying structure which are connected in series; Based on the micro-step structure of the micro-fluidic chip, under the static instability effect of the Rayleigh-Prague, the disperse phase fluid, the intermediate phase fluid and the continuous phase fluid form double emulsion and are conveyed to a double emulsion collecting tank; the image acquisition device acquires a double emulsion image generated at the outlet of the microfluidic chip and transmits the image to the data processing terminal; The built-in trained visual recognition model of the data processing terminal performs instance segmentation on the acquired double emulsion image, recognizes and counts parameters of the double emulsion, and sends out a control instruction to control the pressure pumping device according to a recognition result; the pressure pumping device adjusts the air pressure output value according to the control instruction, so that the particle size of the generated double emulsion is recovered to be within a preset range.
9. The double emulsion detection feedback regulation method of claim 8 wherein the trained visual recognition model uses a mask_ RCNN model.
10. The method for detecting and feedback regulating and controlling double emulsion according to claim 8, wherein the micro-step structure based on micro-fluidic chip forms double emulsion by the disperse phase fluid, the intermediate phase fluid and the continuous phase fluid under the static instability effect of Rayleigh-Prague, and the double emulsion is transported to a double emulsion collecting tank, specifically: When the disperse phase fluid spans the first step emulsion structure, under the action of the step emulsion effect, the disperse phase fluid is split into single emulsion by the intermediate phase fluid and is suspended in the intermediate phase fluid; the intermediate phase fluid wrapping the single emulsion enters a second step emulsifying structure, the intermediate phase fluid wrapping the single emulsion is sheared by the continuous phase fluid under the action of a step emulsifying effect, double emulsion is formed and floats in the continuous fluid, and along with the continuous driving of high-pressure gas, the double emulsion is continuously generated and flows out of an outlet of the microfluidic chip and enters a double emulsion collecting tank.

Description

Double emulsion detection feedback regulation and control system and method Technical Field The invention relates to the technical field of microfluidics, in particular to a double emulsion detection feedback regulation system and method. Background The microfluidic chip is widely applied to aspects of fluid mechanics research, advanced material synthesis, pathogen detection, biomedical analysis and the like by virtue of the characteristics of accurate control of liquid in a microscale channel, high reaction efficiency, and low reagent consumption. The core of the microfluidic control is that the preparation and control of emulsion droplets are carried out, and the emulsion droplets are generally composed of two or more mutually-insoluble liquids, so that the microfluidic control has good dispersibility and controllability. The double emulsion is used as a system formed by multiple layers of liquid drops, not only has better performance in the aspect of packaging stability, but also can effectively isolate the interference of external environment on the content, and has wide function expansion potential. However, the present stage double emulsion formation process still suffers from significant drawbacks. The conventional common preparation strategies such as a T-shaped shearing method, a flow focusing method, a coaxial flow method and the like generally need to manually control the flow rate and the proportion of droplet fluid, and the fluctuation of the flow rate easily causes uneven-sized droplets to seriously influence the monodispersity of droplet products, so that the popularization and the application of the droplet products in biomedical engineering are limited. The device disclosed in publication No. CN113826878B uses a mechanical structure to shear two immiscible liquids at high speed, and although the generation step is simplified to a certain extent, the obtained double emulsion has uneven particle size distribution and poor monodispersity. Another patent of publication No. CN117772020a proposes an assembled two-step double emulsion batch preparation device, but the device needs to rely on frequent disassembly and assembly of different partition boards, which not only has complicated operation, but also has insufficient tightness, and is easy to generate liquid leakage phenomenon, thereby affecting the stability and reliability of double emulsion preparation. Disclosure of Invention In order to solve the problems of uneven particle size control, production stability and the like in the existing double emulsion generation method, the invention provides a double emulsion detection feedback regulation system and method, a mask_ RCNN model is introduced into a feedback control system for double emulsion preparation, a set of closed-loop control system capable of being regulated automatically is constructed, the real-time identification of the size of double emulsion microdroplets and the self-adaptive regulation of the flow rate are realized, and the uniformity and the automation level of microdroplet preparation are improved. In a first aspect, the present invention provides a double emulsion detection feedback regulation system comprising: the liquid storage device is used for storing disperse phase fluid, continuous phase fluid and intermediate phase fluid; The pressure pumping device is communicated with the liquid storage device; the microfluidic chip is communicated with the liquid storage device and comprises a first ladder emulsifying structure and a second ladder emulsifying structure which are connected in series; The double emulsion collecting tank is communicated with the microfluidic chip; the image acquisition device acquires a double emulsion image generated at the outlet of the microfluidic chip; And the data processing terminal is internally provided with a trained visual identification model, and is used for carrying out example segmentation on the double emulsion image acquired by the image acquisition device, identifying and counting parameters of double emulsion microdroplets, and controlling the air pressure output value of the pressure pumping device according to the identification result. In a second aspect, the present invention provides a double emulsion detection feedback regulation method, including: starting a pressure pumping device, and driving the disperse phase fluid, the intermediate phase fluid and the continuous phase fluid in the liquid storage device to enter the microfluidic chip; Based on the micro-step structure of the micro-fluidic chip, under the static instability effect of the Rayleigh-Prague, the disperse phase fluid, the intermediate phase fluid and the continuous phase fluid form double emulsion and are conveyed to a double emulsion collecting tank; the image acquisition device acquires the double emulsion image generated at the outlet of the microfluidic chip in real time and transmits the image to the data processing terminal; The data processing ter