CN-121988214-A - Solution mixing system and solution mixing method

CN121988214ACN 121988214 ACN121988214 ACN 121988214ACN-121988214-A

Abstract

The invention discloses a solution mixing system and a solution mixing method, wherein the solution mixing system comprises at least two reagent pipes, solution selecting pieces and a flow cell, a liquid collecting cavity and a liquid distributing cavity are formed in each solution selecting piece, the liquid distributing cavity is provided with a liquid inlet, a liquid outlet and a liquid collecting port, the liquid collecting port is selectively communicated with any one of the liquid inlet and the liquid outlet, the liquid inlet is communicated with the reagent pipes, the liquid collecting port is communicated with the liquid collecting cavity, the flow cell is communicated with the liquid outlet, and liquid in at least two reagent pipes is suitable for flowing to the flow cell after passing through one or at least two solution selecting pieces. The solution mixing system provided by the invention can be configured at any time when the mixed solution is used, so that the problem of poor stability caused by the fact that the mixed solution is configured in advance and placed for a long time is solved, different solutions can be prepared according to actual conditions, the flexibility of preparing the solutions is high, the sequencing quality can be improved, and the sequencing cost is reduced.

Inventors

YANG CHEN
LU JIAN
LI QIAO
ZHAO WEI
ZHOU MAOSHUN
NIU ZIHUA
XING CHUTIAN
SUN LEILIN

Assignees

深圳华大智造科技股份有限公司

Dates

Publication Date: 20260508
Application Date: 20241107

Claims (10)

1.A solution mixing system, comprising: At least two reagent tubes; The liquid distribution cavity is provided with a liquid inlet, a liquid outlet and a liquid collecting port, the liquid collecting port is selectively communicated with any one of the liquid inlet and the liquid outlet, the liquid inlet is communicated with the reagent tube, and the liquid collecting port is communicated with the liquid collecting cavity; And the flow cell is communicated with the liquid outlet, and the liquid in at least two reagent pipes is suitable for flowing to the flow cell after passing through one or at least two solution selecting pieces.
2. The solution mixing system of claim 1, wherein the number of solution selection elements is one, the number of liquid inlets is one and the number of liquid outlets is two, each reagent tube is communicated with the liquid inlet of the liquid distribution cavity through a selection valve group, the flow cell is communicated with one liquid outlet, and the other liquid outlet is communicated with a preparation tube.
3. The solution mixing system of claim 2, wherein the selector valve block comprises a plurality of three-way valves, one of the three-way valves is a first three-way valve, the first three-way valve is connected to the liquid inlet of the solution selector through a first common line, and two adjacent three-way valves are communicated through a connecting pipe; one of the reagent pipes is connected with the first public pipeline through the first three-way valve, and the other reagent pipes are at least communicated with connecting pipes of two adjacent three-way valves and are connected with the first three-way valve so as to be connected with the first public pipeline through the first three-way valve.
4. The solution mixing system according to claim 1, wherein one solution selecting member is provided, the liquid inlets of one solution selecting member are provided in plurality and the liquid outlets of one solution selecting member are provided in two, each reagent tube is connected to the liquid inlets of the liquid distribution chamber in one-to-one correspondence, the flow cell is communicated with one liquid outlet, and the other liquid outlet is communicated with the preparation tube.
5. The solution mixing system of claim 1, wherein the number of solution selecting members is one, the number of liquid inlets is one, the number of liquid outlets is one, the number of liquid inlets on each solution selecting member is one, the number of liquid outlets on each solution selecting member is one, the number of liquid inlets on each solution selecting member is one, and the liquid inlets on each solution selecting member are connected with the reagent pipe, and the liquid outlets on the solution selecting members are communicated with the flow cell through the main pipeline after being combined.
6. The solution mixing system according to claim 5, wherein a plurality of said liquid outlets of a plurality of said solution selecting members are each joined to said main line through respective transfer lines, and a solution mixing device is provided at a junction of a plurality of said transfer lines or said main line.
7. The solution mixing system according to claim 1, wherein each of the reagent tubes corresponds to one of the reagent lines, one end of each of the reagent lines is communicated with the solution selecting member, and the other end is provided with a reagent needle.
8. A solution mixing method, suitable for use in the solution mixing system of any one of claims 2-3, comprising the steps of: switching the liquid inlet of the liquid distribution cavity to be communicated with the liquid collecting port, and connecting the liquid inlet with the selection valve group; connecting a plurality of reagent tubes with the selection valve set; Switching the selection valve group to enable the liquid inlet to be communicated with one of the reagent pipes, sucking a first reagent, switching the selection valve group to enable the liquid inlet to be communicated with the other reagent pipe, sucking a second reagent, and sucking a plurality of reagents; Switching one liquid outlet to be communicated with the preparation pipe and the liquid collecting port to be communicated with the liquid collecting port, and pumping a mixture of multiple reagents into the preparation pipe; Pumping the mixed liquid from the preparation pipe to the liquid collecting cavity; And switching the other liquid outlet to be communicated with the liquid collecting opening and the flow cell, and pumping the mixed liquid into the flow cell.
9. A solution mixing method, suitable for use in the solution mixing system of claim 4, comprising the steps of: Switching one liquid inlet of the liquid distribution cavity to be communicated with the liquid collection cavity, sucking a first reagent in one reagent tube, switching the other liquid inlet of the liquid distribution cavity to be communicated with the liquid collection cavity, sucking a second reagent in the other reagent tube, and sucking a plurality of reagents; One liquid outlet is communicated with a preparation pipe, mixed liquid of multiple reagents is pumped into the preparation pipe, the mixed liquid in the preparation pipe and the liquid collecting cavity is repeatedly pumped and circulated, and finally the mixed liquid of the preparation pipe is pumped into the liquid collecting cavity; And switching the other liquid outlet to be communicated with the flow cell, and pumping the mixed liquid into the flow cell.
10. A solution mixing method, suitable for use in the solution mixing system of claims 5-6, comprising the steps of: Switching the liquid inlet of one solution selecting piece to be connected with the corresponding reagent tube, sucking the first reagent, switching the liquid inlet of the other solution selecting piece to be connected with the corresponding reagent tube, sucking the second reagent, and sucking a plurality of reagents; And switching the liquid outlets of the solution selection pieces to be connected with the main pipeline, and controlling the solutions in the solution selection pieces to enter the main pipeline simultaneously or sequentially and enter the flow cell through the main pipeline.

Description

Solution mixing system and solution mixing method Technical Field The invention relates to the technical field of biology, in particular to a solution mixing system and a solution mixing method. Background High throughput sequencing techniques typically deliver specific reagents to the flow cell in sequence per sequencing cycle, allowing each template to be extended and sequenced for 1 base per cycle. In the preparation stage before sequencing, the sequencing reagents are generally mixed according to types and prepared in advance. And in the sequencing cycle process, each sequencing cycle sequentially transmits the prepared reagent into a chip to complete a round of reaction, then a detection system is used for detecting the types of bases synthesized in the cycle, and the cycle is repeated until the sequencing length is determined. However, in the prior art, the method of preparing the reagent in advance has the problems that the stability of the mixed solution is poor compared with the stability of each component monomer before mixing, the prepared reagent needs to be used for a plurality of or tens of hours from the beginning of the use of the prepared reagent, the stability of the mixed solution is further deteriorated, the sequencing quality is affected, only a single concentration solution can be realized, and the concentration cannot be adjusted according to the actual situation. Disclosure of Invention The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the invention provides a solution mixing system and a solution mixing method, wherein the solution mixing system is configured at any time when the mixed solution is used, so that the problem of poor stability caused by the fact that the mixed solution is configured in advance and placed for a long time is solved, different solutions can be prepared according to actual conditions, the flexibility of preparing the solutions is high, the sequencing quality can be improved, and the sequencing cost is reduced. The solution mixing system comprises at least two reagent pipes and a solution selecting piece, wherein a liquid collecting cavity and a liquid distributing cavity are formed in the solution selecting piece, the liquid distributing cavity is provided with a liquid inlet, a liquid outlet and a liquid collecting opening, the liquid collecting opening is selectively communicated with any one of the liquid inlet and the liquid outlet, the liquid inlet is communicated with the reagent pipes, the liquid collecting opening is communicated with the liquid collecting cavity, the flow cell is communicated with the liquid outlet, and liquid in at least two reagent pipes is suitable for flowing to the flow cell after passing through one or at least two solution selecting pieces. According to the solution mixing system provided by the embodiment of the invention, the liquid distribution cavity of the solution selection piece is communicated with different reagent pipes, namely different solutions can be arranged in the different reagent pipes, the solutions in the different reagent pipes are sucked into the liquid collecting cavity in the solution selection piece for mixing, the solutions in the liquid collecting cavity flow to the flow tank through the liquid outlet after mixing, the flow tank is used in real time, in the process of supplying the solutions to the flow tank, the concentration in one of the reagent pipes which is possibly needed in the early stage is lower, the concentration of the mixed solution in the early stage is high, the concentration of the mixed solution in the early stage can be supplied according to actual needs, and more solutions in the reagent pipes can be extracted along with the needs in the later stage. According to the solution mixing system provided by the embodiment of the invention, one solution selecting piece is arranged, the number of the liquid inlets of the solution selecting piece is one, the number of the liquid outlets of the solution selecting piece is two, each reagent pipe is communicated with the liquid inlet of the liquid distribution cavity through the selecting valve group, the flow cell is communicated with one liquid outlet, and the other liquid outlet is communicated with the preparation pipe. According to the solution mixing system provided by the embodiment of the invention, the selection valve group comprises a plurality of three-way valves, one of the three-way valves is a first three-way valve, the first three-way valve is connected with the liquid inlet of the solution selection part through a first public pipeline, two adjacent three-way valves are communicated through connecting pipes, one reagent pipe is connected with the first public pipeline through the first three-way valve, and the other reagent pipes are at least communicated with the connecting pipes of the two adjacent three-way valves and are connected with the first three-way va