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CN-121990687-A - Method for removing ammonia nitrogen and nitrite in aquaculture water in fish fry

CN121990687ACN 121990687 ACN121990687 ACN 121990687ACN-121990687-A

Abstract

The invention discloses a method for removing ammonia nitrogen and nitrite in a culture water body in fish fry. The method comprises the steps of setting a water sample for cultivation with the C/N of 15-25, stocking healthy rheum officinale fries, collecting bacterial liquid C when the bacterial liquid A or B reaches the OD value of about 5, adding the bacterial liquid A or B or C into a fry cultivation system with the C/N value of 15 for sucking dirt and changing water in the morning and normally feeding feed, collecting the water sample about 10 days later, inoculating the water sample into a denitrification enrichment culture medium for cultivation, transferring after about 5 days, repeating the steps for a plurality of times, and harvesting bacterial liquid A or B after the total cultivation for about 5 days, transferring the fries into the sea for cultivation after the bacterial liquid A or B or C is fed for more than 21 days, and expanding the bacterial liquid A or B to the OD value of about 5, collecting the bacterial liquid C, adding the bacterial liquid A or B or C into the fry cultivation system with the C/N value of 15 for sucking dirt and changing water. And simultaneously, ammonia nitrogen and nitrite indexes in the culture water body in fish fry raising can be removed.

Inventors

  • SUN QIAN
  • Zeng Qiaoting
  • QIN DAN

Assignees

  • 中国科学院城市环境研究所

Dates

Publication Date
20260508
Application Date
20241105

Claims (6)

  1. 1. A method for removing ammonia nitrogen and nitrite in a culture water body in fish fry rearing is characterized by comprising the following steps, S1, setting a culture water sample with the C/N of 15-25, stocking rhubarb fish fries with healthy constitution and good vitality, controlling the water temperature to be 25 ℃ during the test, carrying out sewage absorption and water changing in noon every day, wherein the sewage absorption amount is the water changing amount of each group, and feeding feed normally in the morning and afternoon every day; Or if the bacterial liquid A is obtained by culturing a plurality of different C/N, collecting the harvested bacterial liquid A, culturing for 5+/-1 days, transferring, culturing repeatedly for 1-5 times, and collecting the bacterial liquid B; s2, expanding culture of the harvested bacterial liquid A or bacterial liquid B until the OD value is 5.0+/-0.5, collecting bacterial liquid C, and storing at low temperature to keep the bacterial liquid alive; S3, adding the collected bacterial liquid A or bacterial liquid B or bacterial liquid C into a fish fry cultivation system with the C/N value of 15 and the water exchange amount of which is equal to the sewage absorption amount every day, and transferring the fish fry into the sea for cultivation after feeding for more than 21 days.
  2. 2. The method of claim 1, wherein in steps S1 and S3, the fed basal diet has an average crude protein content of ∈47.0%, crude fat content of ∈5.0%, crude fiber content of ∈2.0%, crude ash content of ∈16.0%, calcium content of ∈5.0%, lysine content of ∈2.1%, and total phosphorus content of ∈1.2%.
  3. 3. The method of claim 1, wherein in step S1, the transfer amount per transfer is 1:10 by volume.
  4. 4. The method of claim 1, wherein in the step S1, the number of repeated transfers is 2.
  5. 5. The method according to claim 1, wherein in the step S3, the ratio of the bacterial liquid a or the bacterial liquid B or the bacterial liquid C to the culture water body is 1ml to 0.5t, or the ratio of the bacterial liquid a or the bacterial liquid B or the bacterial liquid C to the culture fish fry is 1ml to 1000 fish.
  6. 6. The method of claim 1, wherein in step S3, the fry is a large yellow croaker fry.

Description

Method for removing ammonia nitrogen and nitrite in aquaculture water in fish fry Technical Field The invention relates to the field of cultivation, in particular to a method for removing ammonia nitrogen and nitrite in a cultivation water body in fish fry. Background The large yellow croaker (LARIMICHTHYS CROCEA) belongs to the genus Talargehead family (SCIAENIDAE), the large yellow croaker belongs to the genus Talargehead (LARIMICHTHYS), and is the sea water farmed fish with the maximum standard in China, the yield in 2020 reaches 25.4 ten thousand tons, and the annual seedling raising amount exceeds 30 hundred million. The large yellow croaker seedling is mainly raised in spring, and the sea water with more than 10 degrees in the sea area is heated to more than 25 degrees to meet the requirement of large yellow croaker seedling. As the large yellow croaker adopts the industrial high-density seedling raising technology, the feeding amount and the water changing amount are large, tens of thousands of tons of heated seedling raising waste water are discharged into the sea every day during the seedling raising period, and the phenomena of energy waste and environmental pollution are prominent. Ammonia nitrogen is an important metabolite of the aquaculture animals, and accumulation of ammonia nitrogen and nitrite in the aquaculture water area can cause harm to the aquaculture animals. One of the key points of the application of the biological flocs in the large yellow croaker culture technology is that functional flora capable of cooperatively treating pollution indexes such as ammonia nitrogen, nitrite nitrogen and the like in a culture water body is domesticated, so that the water exchange amount is reduced, and the culture cost is saved. The biological flocculation technology is characterized in that organic carbon substances are artificially added into a culture water body, the carbon nitrogen ratio (C/N) in the water body is regulated, the quantity of heterotrophic bacteria in the water body is increased, and nitrogen-containing compounds such as ammonia nitrogen and the like in the water body are converted into bacterial proteins by utilizing the assimilation of inorganic nitrogen by microorganisms, so that the biological flocculation capable of being directly ingested by a cultured object is formed. The functional flora after enrichment culture can be used for field planting culture in a seedling culture water body, is beneficial to promoting the application of a biological flocculation technology to be better applied to large yellow croaker seedling culture, is beneficial to removing ammonia nitrogen and nitrite nitrogen in the culture water body in large yellow croaker seedling culture, and promotes energy conservation and emission reduction of industry. Disclosure of Invention The invention aims to provide a method for removing ammonia nitrogen and nitrite in a culture water body in fish fry. The functional flora enrichment culture of the invention can be used for field planting culture in a water body for culturing fish, and aims to remove indexes such as ammonia nitrogen, nitrite and the like in the water body for culturing fish. In order to achieve the aim, the invention provides a method for removing ammonia nitrogen and nitrite in a culture water body in fish fry rearing, which is characterized by comprising the following steps, S1, setting a culture water sample with the C/N of 15-25, stocking rhubarb fish fries with healthy constitution and good vitality, controlling the water temperature to be 25 ℃ during the test, carrying out sewage absorption and water changing in noon every day, wherein the sewage absorption amount is the water changing amount of each group, and feeding feed normally in the morning and afternoon every day; Or if the bacterial liquid A is obtained by culturing a plurality of different C/N, collecting the harvested bacterial liquid A, culturing for 5+/-1 days, transferring, culturing repeatedly for 1-5 times, and collecting the bacterial liquid B; s2, expanding culture of the harvested bacterial liquid A or bacterial liquid B until the OD value is 5.0+/-0.5, collecting bacterial liquid C, and storing at low temperature to keep the bacterial liquid alive; s3, adding the collected bacterial liquid A or bacterial liquid B or bacterial liquid C into a fish fry cultivation system with the C/N value of 15 and the water exchange amount of which is equal to the sewage absorption amount every day, and transferring the fish fry into the sea for cultivation after feeding for more than 14 days. Further, in the steps S1 and S3, the average crude protein content of the fed basic culture feed is not less than 47.0%, the crude fat content is not less than 5.0%, the crude fiber content is not less than 2.0%, the crude ash content is not less than 16.0%, the calcium content is not less than 5.0%, the lysine content is not less than 2.1%, and the total phosphorus content is not less than 1.2