CN-121990898-A - Siraitia grosvenorii abietane diterpenoid compound separated from pinus massoniana, and preparation method and application thereof

Abstract

The invention relates to the technical field of natural product separation, in particular to a momordica grosvenori abietane diterpenoid compound separated from a pinus massoniana pinecone, a preparation method and application thereof, the preparation method of the momordica grosvenori alkane diterpenoid compound comprises the steps of extracting pinus massoniana with alcohol, adsorbing with resin and separating with column to obtain the momordica grosvenori alkane diterpenoid compound. The momordica grosvenori alkane diterpenoid compound can be used for resisting inflammation, and has certain inhibitory activity on RAW264.7 cells.

Inventors

• LI YONGJUN
• LI YAO
• CHEN MINGYUE
• ZI ZHENYU
• JIANG LI
• WANG YANG
• MA XUE
• SUN JIA

Assignees

• 贵州医科大学

Dates

Publication Date

20260508

Application Date

20260331

Claims (9)

1. 1. The momordica grosvenori abietane diterpenoid compound separated from the pinus massoniana, which is characterized by having a structure shown in a formula 1: 。
2. 2. the momordica grosvenori abietane diterpenoid compound separated from the pinus massoniana, which is characterized by having a structure shown in a formula 2: 。
3. 3. the momordica grosvenori abietane diterpenoid compound separated from the pinus massoniana, which is characterized by having a structure shown in a formula 3: 。
4. 4. The preparation method of the momordica grosvenori alkane diterpenoid compound separated from the pinus massoniana pine cone is characterized by comprising the steps of carrying out alcohol extraction, macroporous resin adsorption and column separation on the pinus massoniana pine cone to obtain a compound 1-3; The compounds 1-3 have structures as shown in formulas 1 to 3: 。
5. 5. The method for preparing a momordica grosvenori alkane diterpenoid compound separated from a pinus massoniana pine cone according to claim 4, wherein an extract of pinus massoniana pine through alcohol extraction is adsorbed by macroporous adsorption resin, ethanol is eluted and concentrated to obtain an ethanol section, the ethanol section is separated by a column to obtain 10 components Fr.1-10, fr.4 is separated by a column to obtain 7 components Fr.4.1-4.7, fr.4.3 is separated by a column to obtain a compound 1, fr.6 is separated by a column to obtain 10 components Fr.6.1-6.10, fr.6.5 is separated by a column to obtain 7 components Fr.6.5.1-6.5.7, fr.6.5.5 is separated by a column to obtain a compound 2, and Fr.6.5.4 is separated by a column to obtain a compound 3.
6. 6. A pharmaceutical composition consisting of a luohan abietane diterpenoid compound of any one of claims 1-3 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier or excipient.
7. 7. An inhibitor comprising the momordica grosvenori alkane diterpenoid compound according to any one of claims 1 to 3.
8. 8. Use of a luohan rosin alkane diterpenoid compound according to any one of claims 1-3 in the preparation of an anti-inflammatory inhibitor.
9. 9. Use of a luohan rosin alkane diterpenoid compound according to any one of claims 1-3 in the preparation of a RAW264.7 cell inhibitor.

Description

Siraitia grosvenorii abietane diterpenoid compound separated from pinus massoniana, and preparation method and application thereof Technical Field The invention relates to the technical field of natural product separation, in particular to a momordica grosvenori abietane diterpenoid compound separated from a pinus massoniana pinecone, and a preparation method and application thereof. Background Pinus massoniana is the mature fruit of Pinus massoniana of Pinaceae, and is the cone of Pinaceae, also called pine cone, and pine cone. The main chemical components are polysaccharide, terpenes, flavonoids, polyphenol, lignin, volatile oil and the like, and the chemical components are various and complex. Pinus massoniana pine cone has the effect of relieving cough and asthma, and is used for treating cough and asthma phlegm retention, chronic bronchitis and the like. Clinical data shows that pine cones have anti-inflammatory, anti-tumor, antibacterial and antioxidant effects, and that these potential effects are of increasing interest, hopefully exerting their greater value. Pinus massoniana pinecone exhibits various potential effects in clinical treatment, such as relieving cough, enteritis, neurasthenia, and the like, and has antiviral infection, antitumor, anti-inflammatory (treating chronic bronchitis) and the like. These effects make pinecone a natural drug resource of great interest. In addition, pinus massoniana pine cone also has the functions of enhancing immunity, resisting oxidation and the like, and is widely applied in traditional medicine, for example, the functions of relieving cough, relieving asthma, dispelling wind, lubricating intestines and the like of the Pinus massoniana are recorded in ancient books such as Ben Cao gang mu and the like. Modern research also shows that the polyphenols in Pinus massoniana pine cone are associated with lower cancer incidence, and that their anti-tumor mechanisms may include activation of apoptosis pathways, termination of cell cycle, etc. Therefore, the development and utilization of the pinus massoniana pine cone have wide prospects, and deserve further intensive research to fully exert the medicinal value. Inflammation is a protective response of the body to tissue injury, infection and abnormal conditions, and has the effects of removing harmful substances, repairing damaged tissues, regulating immune functions and the like, and excessive inflammatory response can lead to tissue injury and dysfunction of patients. RAW264.7 macrophages play a complex and critical role in the development, progression and maintenance of homeostasis of inflammation by secreting pro-inflammatory cytokines and regulating the state of polarization. Disclosure of Invention It is an object of the present invention to provide a siraitia grosvenorii-type diterpenoid compound separated from a pinus massoniana, another object of the present invention is to provide a method for obtaining an effective chemical component from a pinus massoniana, and to provide an application of the siraitia grosvenorii-type diterpenoid compound. According to a first aspect of the present invention, there is provided a momordica grosvenori abietane diterpenoid compound isolated from the inside of a pinus massoniana pine cone, the compound having a structure as shown in formula 1: 。 according to a second aspect of the present invention, there is provided a momordica grosvenori abietane diterpenoid compound isolated from the inside of Pinus massoniana, the compound having a structure as shown in formula 2: 。 according to a third aspect of the present invention, there is provided a momordica grosvenori abietane diterpenoid compound isolated from the inside of Pinus massoniana, the compound having a structure as shown in formula 3: 。 According to a fourth aspect of the present invention, there is provided a process for the preparation of a momordica rosin alkane diterpenoid compound isolated from the interior of Pinus massoniana, which comprises subjecting Pinus massoniana to alcohol extraction, macroporous resin adsorption and column separation to obtain compounds 1 to 3; The compounds 1-3 have structures as shown in formulas 1 to 3: 。 optionally, the extract of the pinus massoniana pine through alcohol extraction is adsorbed by macroporous adsorption resin, ethanol is eluted and concentrated to obtain an ethanol section, the ethanol section is separated by a column to obtain 10 components Fr.1-10, fr.4 is separated by the column to obtain 7 components Fr.4.1-4.7, fr.4.3 is separated by the column to obtain a compound 1, fr.6 is separated by the column to obtain 10 components Fr.6.1-6.10, fr.6.5 is separated by the column to obtain 7 components Fr.6.5.1-6.5.7, fr.6.5.5 is separated by the column to obtain a compound 2, and Fr.6.5.4 is separated by the column to obtain a compound 3. Alternatively, separating fr.4.3 by column to obtain compound 1 comprises eluting fr.4.3 by gel column with methanol to obtain 2 com