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CN-121990974-A - Organic fluorescent probe, application of organic fluorescent probe in observing or measuring solution viscosity and viscosity detection device

CN121990974ACN 121990974 ACN121990974 ACN 121990974ACN-121990974-A

Abstract

The invention discloses an organic fluorescent probe, application of the organic fluorescent probe in observing or measuring solution viscosity and a viscosity detection device, wherein the organic fluorescent probe has the following structural formula: r is iodoethanol or methyl. The organic fluorescent probe can observe the solution viscosity by detecting the change of the solution absorbance of the organic fluorescent probe through a viscosity response mechanism, and can calculate the solution viscosity by a fluorescence intensity value so as to realize the trace analysis of the solution viscosity detection.

Inventors

  • WEI MENGMENG
  • YANG RUI
  • ZHENG YUBIN
  • XU JING

Assignees

  • 苏州瀚钠材料科技有限公司

Dates

Publication Date
20260508
Application Date
20251216

Claims (9)

  1. 1. An organic fluorescent probe is characterized by having a structural formula as follows: R is iodoethanol or methyl.
  2. 2. The organic fluorescent probe of claim 1, wherein the synthetic route of the organic fluorescent probe is: , The compound 1 and the compound 2 are reacted to synthesize the organic fluorescent probe through Knoevenagel, the mass ratio of the compound 1 to the compound 2 is 1 (1-1.2), the temperature is 85-95 ℃, the reflux time is 6-10 h, the catalyst in the Knoevenagel reaction is organic alkali, and the generated organic fluorescent probe is purified through column chromatography.
  3. 3. The use of the organic fluorescent probe according to claim 1 for observing the solution viscosity by an absorbance method, wherein the organic fluorescent probe is added into a solution to be measured for dissolution, and the solution viscosity is observed by observing the magnitude of the absorbance of the solution.
  4. 4. The use of the organic fluorescent probe according to claim 1 for measuring the viscosity of a solution, wherein the organic fluorescent probe is added into the solution to be measured for dissolution, then the fluorescence intensity I F of the solution is detected, and then the viscosity eta of the solution is calculated according to an equation, wherein the viscosity eta of the solution is more than or equal to 1cP.
  5. 5. The use according to claim 4, wherein the viscosity of the solution is in the range of 1cP to 1000cP.
  6. 6. The use according to claim 4, wherein the concentration of the organic fluorescent probe in the solution is 5-15 μm.
  7. 7. The use according to claim 6, wherein the concentration of the organic fluorescent probe in the solution is 10 μm.
  8. 8. A viscosity detection device for use according to any one of claims 3-7, wherein the viscosity detection device is a fluorescence spectrometer comprising a memory and a processor, wherein the memory stores a calculation program which, when executed by the processor, is capable of performing the step of bringing the measured fluorescence intensity I F of the solution into the equation log I F = 1.95+0.86log eta, and calculating the viscosity eta of the solution.
  9. 9. A viscosity detection device for use according to any one of claims 4-7, comprising a fluorescence spectrometer and a peripheral controller electrically connected to the fluorescence spectrometer, the peripheral controller comprising a memory and a processor, wherein the fluorescence spectrometer is adapted to determine the fluorescence intensity I F of a solution and to communicate the fluorescence intensity I F to the peripheral controller, wherein the memory stores a calculation program which, when executed by the processor, is adapted to carry out the step of bringing the fluorescence intensity I F of the solution into the equation log I F = 1.95+0.86log η to calculate the viscosity η of the solution.

Description

Organic fluorescent probe, application of organic fluorescent probe in observing or measuring solution viscosity and viscosity detection device Technical Field The invention belongs to the field of fluorescent probes, and particularly relates to an organic fluorescent probe, application of the organic fluorescent probe in observing or measuring solution viscosity and a viscosity detection device. Background Viscosity is a physical quantity for measuring the deformation resistance of a fluid, reflects the characteristic of internal friction generated in the fluid during flowing, and is also a key index for evaluating the quality of partial finished products or semi-finished products. The current mainstream viscosity detection method is mainly a viscometer method, and common instruments comprise a falling ball type viscometer, a rotary viscometer and the like. The detection method generally needs to be in direct contact with the solution to be detected, so that the solution consumption in the detection process is large, and the solution is difficult to recycle after being contacted by the viscometer, so that development of a liquid viscosity detection technology which does not need to be in contact with the solution and only needs a trace amount of the solution to be detected is needed. The fluorescence detection technology is used as a microanalysis means, has the advantages of high sensitivity, strong selectivity, simple operation, quick response and the like, and is an effective way for realizing microanalysis based on solution viscosity detection of a fluorescence probe. Currently, fluorescent probe studies on viscosity response have focused on qualitative determination of solution viscosity level by fluorescence intensity variation. For example, the invention patent with publication number CN113845462 discloses a fluorescent probe molecule composed of indole and indole iodized salt, which can respond to the viscosity of the solution, and can qualitatively judge the viscosity of the solution through the change of fluorescence intensity, but does not involve the realization of the quantitative analysis function. In addition, the observation of solution viscosity by absorbance methods is rarely reported, which is detrimental to viscosity detection. Disclosure of Invention The invention mainly solves the technical problem of providing an organic fluorescent probe, application of the organic fluorescent probe in observing or measuring solution viscosity and a viscosity detection device, wherein the organic fluorescent probe can observe the solution viscosity by detecting the change of the solution absorbance of the organic fluorescent probe through a viscosity response mechanism, and can calculate the solution viscosity through a fluorescence intensity value so as to realize the trace analysis of the solution viscosity detection. In order to solve the technical problems, the invention adopts a technical scheme that an organic fluorescent probe is provided, and the structural formula of the organic fluorescent probe is as follows: R is iodoethanol or methyl. In a preferred embodiment of the present invention, the synthetic route of the organic fluorescent probe is as follows: , The compound 1 and the compound 2 are reacted to synthesize the organic fluorescent probe through Knoevenagel, the mass ratio of the compound 1 to the compound 2 is 1 (1-1.2), the temperature is 85-95 ℃, the reflux time is 6-10 h, the catalyst in the Knoevenagel reaction is organic alkali, and the generated organic fluorescent probe is purified through column chromatography. The application of observing the solution viscosity by an absorbance method based on the organic fluorescent probe is that the organic fluorescent probe is added into a solution to be measured for dissolution, and the solution viscosity is observed by observing the absorbance of the solution. The application of measuring the solution viscosity based on the organic fluorescent probe comprises the steps of adding the organic fluorescent probe into a solution to be measured for dissolution, detecting the fluorescence intensity I F of the solution, and calculating the viscosity eta of the solution according to an equation, wherein the viscosity eta of the solution is more than or equal to 1cP. The viscosity of the solution to be measured is lower than 1cP, the accuracy of measuring the viscosity of the solution is low, the solubility of the organic fluorescent probe can be possibly reduced in a solvent smaller than 1cP due to the long-chain structure of the organic fluorescent probe, the concentration in the solution is too small, the fluorescence intensity can be reduced, and the measurement error is increased. In a preferred embodiment of the invention, the viscosity of the solution ranges from 1cP to 1000cP. In a preferred embodiment of the invention, the concentration of the organic fluorescent probe in the solution is 5-15 mu M, and when the concentration of the or