CN-121991172-A - Bioactive peptide with oral cavity anti-aging effect and application thereof

Abstract

The embodiment of the invention discloses a bioactive peptide with an oral cavity anti-aging effect and application thereof. According to the invention, through a series of experiments, the bioactive polypeptides IR-11 and PK-11 have remarkable anti-inflammatory effect, barrier repairing effect and antioxidant and anti-aging effects, can be applied to prevention or treatment of chronic inflammatory oral diseases and oral debilitation represented by periodontitis, and have good development and application prospects.

Inventors

• XU JUAN
• LI LIXIAN
• ZHANG ZHIWEI
• LI ZHIYIN
• MA SU
• HUANG YUMIN
• Dai Piaopiao

Assignees

• 云南白药集团股份有限公司
• 云南白药集团健康产品有限公司

Dates

Publication Date

20260508

Application Date

20260313

Claims (10)

1. 1. The bioactive peptide with the oral cavity anti-aging effect is characterized in that the amino acid sequence is shown as SEQ ID NO. 2.
2. 2. A bioactive peptide derivative having an oral anti-aging effect, wherein the bioactive peptide derivative is obtained by modifying an amino acid side chain group, an amino terminal or a carboxyl terminal of the bioactive peptide of claim 1 by hydroxylation, carboxylation, carbonylation, methylation, acetylation, phosphorylation, esterification or glycosylation, and the bioactive peptide derivative retains the biological activity of the bioactive peptide.
3. 3. The bioactive peptide derivative according to claim 2, characterized in that the modification is palmitoylation modification of the N-terminus of the bioactive peptide.
4. 4. A composition comprising the bioactive peptide of claim 1, or the bioactive peptide derivative of claim 2 or 3, and an acceptable carrier.
5. 5. The composition of claim 4, wherein the acceptable carrier comprises a filler, binder, disintegrant, lubricant, solvent, suspending agent, emulsifier, pH adjuster, abrasive, humectant, thickener/viscosity adjuster, foaming agent, taste modifier, appearance modifier, preservative, antioxidant, fragrance, cleanser.
6. 6. The composition of claim 4, wherein the dosage form comprises an oral dosage form, a parenteral dosage form, a buccal dosage form, a sublingual dosage form, a nasal dosage form, a transdermal dosage form, and a suppository.
7. 7. Use of a bioactive peptide according to claim 1, a bioactive peptide derivative according to claim 2 or 3, a composition according to any one of claims 4 to 6 or a bioactive peptide having the amino acid sequence shown in SEQ ID No. 1 for the preparation of a product for preventing, alleviating or treating periodontal problems, including gingivitis and periodontitis, in cosmetics, oral care products, disinfection products, medical devices or medicaments.
8. 8. The use according to claim 7, wherein the product is used for promoting gingival tissue repair, combating gingival recession, repairing black triangles, promoting gingival collagen production, tightening periodontal gums, reducing periodontal pocket formation or preventing alveolar bone resorption.
9. 9. The use according to claim 7, wherein the product comprises an anti-oxidant anti-inflammatory gingival preparation, an oral care toothpaste, an oral care gel, an oral care essence, a mouthwash, an oral spray, an oral care patch, a dental Zhou Huli paste, a desensitizing paste, a periodontal injection.
10. 10. Use of the bioactive peptide of claim 1, the bioactive peptide derivative of claim 2 or 3, the composition of any one of claims 4-6, or the bioactive peptide having the amino acid sequence shown in SEQ ID No. 1 for preparing a product having anti-inflammatory efficacy comprising significantly inhibiting NO content, significantly inhibiting TNF- α content, barrier repair efficacy comprising increasing cell viability, barrier repair efficacy comprising significantly inhibiting ROS superoxide production, and oxidation resistance anti-aging efficacy.

Description

Bioactive peptide with oral cavity anti-aging effect and application thereof Technical Field The embodiment of the invention relates to the technical field of biological medicine and cosmetics, in particular to a bioactive peptide with an oral cavity anti-aging effect and application thereof. Background The American cockroach (PERIPLANETA AMERICANA) is used as a main source of the traditional Chinese medicinal material cockroach and has a long medicinal history. At present, chinese patent medicines such as a rehabilitation new liquid, a heart vein injection and the like which take the periplaneta americana extract as main components are widely applied to the auxiliary treatment of diseases such as wound repair, peptic ulcer, chronic heart failure and the like clinically. However, the preparation prepared by the traditional extraction process has complex ingredients and large batch difference, lacks attention to single active ingredients, and limits the popularization of the preparation in international medicine registration and high-standard pharmacological mechanism research. With the development of multiple groups of chemical analysis, peptide fingerprint and biological function detection technologies, a research system taking 'polypeptide enrichment grading-fingerprint characterization-function screening' as a core is established, and screening of small molecule polypeptide groups with definite structural and functional characteristics becomes a key direction for improving the quality consistency and the drug effect controllability of the American cockroach extract preparation. Through structural confirmation, functional verification and pharmacodynamic association analysis of the characteristic polypeptide components, the conversion from 'empirical extract' to 'standardized active polypeptide preparation' is hopeful to be realized, and the application of the polypeptide preparation in the fields of wound repair, anti-infection, tumor treatment related injury repair and the like is expanded. Disclosure of Invention Therefore, the embodiment of the invention provides the bioactive peptide with the oral cavity anti-aging effect and the application thereof. In order to achieve the above object, the embodiment of the present invention provides the following technical solutions: according to a first aspect of embodiments of the present invention, there is provided a bioactive peptide having oral cavity anti-aging effect, the amino acid sequence of which is shown in SEQ ID NO.2 (PIIEDYHGGFK). According to a second aspect of embodiments of the present invention, there is provided a bioactive peptide derivative having an oral anti-aging effect, the bioactive peptide derivative being obtained by modifying an amino acid side chain group, amino terminal or carboxyl terminal of the bioactive peptide as described above by hydroxylation, carboxylation, carbonylation, methylation, acetylation, phosphorylation, esterification or glycosylation, and the bioactive peptide derivative retaining the biological activity of the bioactive peptide. Further, the modification is palmitoylation modification of the N-terminus of the bioactive peptide. According to a third aspect of embodiments of the present invention, there is provided a composition comprising a bioactive peptide as described above, or a bioactive peptide derivative as described above, and an acceptable carrier. Further, the pharmaceutically acceptable carrier includes, but is not limited to, fillers, binders, disintegrants, lubricants, solvents, suspending agents, emulsifiers, pH adjusters, abrasives, humectants, thickeners/viscosity modifiers, foaming agents, taste modifiers, appearance modifiers, preservatives, antioxidants, fragrances, cleansing agents. As examples, the filler may be lactose, sucrose, potato starch, microcrystalline cellulose, sodium carboxymethyl cellulose, etc., the binder may be gelatin, acacia, povidone, sodium carboxymethyl cellulose, hydroxyethyl cellulose, carbopol resin, etc., the disintegrant may be crospovidone, sodium carboxymethyl starch, etc., the lubricant may be magnesium stearate, calcium stearate, talc powder, micropowder silica gel, polyethylene glycol, etc., the solvent may be aqueous solvent such as purified water, water for injection, suspending agent may be carbomer, acacia, etc., the emulsifier may be sodium dodecyl sulfate, tween, etc., the pH regulator may be citric acid, sodium bicarbonate, etc., the friction agent may be silicon dioxide, calcium carbonate, calcium hydrogen phosphate, etc., the humectant may be glycerin, propylene glycol, etc., the thickener/viscosity regulator may be sodium carboxymethyl cellulose, etc., the foaming agent may be sodium dodecyl sulfate, alkyl glycoside, etc., the taste modifier may be sweetener, etc., the appearance improver may be methyl orange, suspending agent may be sodium chloride, etc., the preservative may be sodium chloride, the antioxidant may be sodium chloride, etc., the anti