CN-121991173-A - Antibacterial peptide derived from bullfrog skin and application thereof

Abstract

The invention discloses an antibacterial peptide derived from bullfrog skin and application thereof, wherein the amino acid sequence of the antibacterial peptide is GSVGPVGPR (shown as SEQ ID NO: 1), and the molecular weight is 824.4504 g/mol. The antibacterial peptide has obvious inhibition effect on staphylococcus aureus, and can be used for preparing medicines for preventing or inhibiting staphylococcus aureus infection. Meanwhile, when the antibacterial peptide is combined with the vitamin A, the antibacterial peptide has obvious synergistic bactericidal effect on staphylococcus aureus, and the combined use of the antibacterial peptide and the vitamin A can obviously reduce the use concentration of single components, improve the antibacterial efficiency, overcome the problem of bacterial drug resistance and can be used for preparing antibacterial drugs, skin external preparations, medical dressings, feed additives and food preservatives.

Inventors

• WANG ZHONGYAN
• LI WU
• XIAO FENG
• WU GEHUA

Assignees

• 广州爱保农生物科技有限公司

Dates

Publication Date

20260508

Application Date

20260401

Claims (10)

1. 1. An antibacterial peptide derived from bullfrog skin, which is characterized in that the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 1.
2. 2. A nucleic acid encoding the antimicrobial peptide of claim 1.
3. 3. An expression vector comprising the nucleic acid of claim 2.
4. 4. A recombinant cell comprising the expression vector of claim 3.
5. 5. Use of an antibacterial peptide or a nucleic acid encoding the same according to claim 1 for the preparation of an antibacterial drug, a feed additive or a food preservative.
6. 6. The use according to claim 5, wherein the antibacterial agent, feed additive or food preservative is for inhibiting and/or killing staphylococcus aureus.
7. 7. An antibacterial drug, which is characterized in that, the antibacterial drug comprises the antibacterial peptide of claim 1.
8. 8. The antibacterial agent according to claim 7, wherein, the antibacterial drug also comprises vitamin A.
9. 9. A feed additive is characterized in that, the feed additive comprising the antibacterial peptide of claim 1.
10. 10. The feed additive of claim 9 wherein the feed additive further comprises vitamin a.

Description

Antibacterial peptide derived from bullfrog skin and application thereof Technical Field The invention relates to the technical field of biology, in particular to an antibacterial peptide derived from bullfrog skin and application thereof. Background Staphylococcus aureus (Staphylococcus aureus) is a widely occurring gram-positive pathogen that can cause a variety of diseases ranging from mild skin infections to life threatening bacteremia, pneumonia, etc. With the advent of multidrug resistant strains such as methicillin-resistant staphylococcus aureus (MRSA), the clinical treatment is facing a great challenge, and development of novel antimicrobial strategies that are not prone to induce drug resistance is highly desirable. Antibacterial peptides (AMPs) are a class of multifunctional small molecule proteins known for their broad spectrum antibacterial, antiviral and antifungal activity. These peptides are ubiquitous in a variety of life forms, including animals, plants and microorganisms, and are of great interest to the scientific community due to their unique mechanism of action and potential use. Unlike traditional antibiotics, AMPs primarily target bacterial cell membranes, destroy them by multiple pathways, and eliminate bacteria by multiple targets, which greatly reduces the likelihood of bacterial resistance development. AMPs have found wide application as a replacement for traditional antibiotics in medicine, animal husbandry, and the food industry. The prior researches show that the retinoic acid with high concentration (generally more than 30 mug/mL) has direct antibacterial effect on certain bacteria (such as propionibacterium acnes and staphylococcus aureus), but has higher effective concentration and limited single application value. Therefore, finding a novel antibacterial peptide which can inhibit and kill staphylococcus aureus without generating drug resistance and can be combined with vitamin A (retinoic acid) to improve antibacterial efficiency is a technical problem to be solved in the industry. Disclosure of Invention The invention aims to overcome the defects of the prior art, provides an antibacterial peptide derived from bullfrog skin and application thereof, and solves the problems in the prior art. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: One of the technical schemes adopted for solving the technical problems is to provide an antibacterial peptide derived from bullfrog skin, wherein the amino acid sequence of the antibacterial peptide is GSVGPVGPR (shown as SEQ ID NO: 1), the molecular weight of the antibacterial peptide is 824.4504 daltons, the positive charge is +1, the total hydrophobicity ratio is 22%, and the peptide is predicted to be of a random structure through APD 3. The invention also provides nucleic acids encoding the above antimicrobial peptides, expression vectors containing the nucleic acids, and recombinant cells containing the expression vectors. The invention also provides application of the antibacterial peptide or the encoding nucleic acid thereof in preparation of antibacterial drugs or feed additives. Preferably, the antibacterial agent or feed additive is used for inhibiting and/or killing staphylococcus aureus. The second technical scheme adopted for solving the technical problem is to provide an antibacterial drug, wherein the antibacterial drug comprises the antibacterial peptide. Preferably, the antibacterial agent further comprises vitamin a. The third technical scheme adopted for solving the technical problems is to provide a feed additive, wherein the feed additive comprises the antibacterial peptide. Preferably, the feed additive further comprises vitamin a. The antibacterial peptide of the invention mainly acts on bacterial cell membranes, the permeability of the cell membranes is increased, the leakage of ions, metabolites and other cell contents in the cells is caused, the cell membranes lose integrity, the membrane structure and functions are destroyed, and the like, and the changes finally lead to the death of bacterial cells. The antimicrobial peptides of the invention can be synthesized using methods known to those skilled in the art, such as solid phase synthesis, and purified using methods known to those skilled in the art, such as high performance liquid chromatography. The implementation of the invention has the following beneficial effects: 1. The antibacterial peptide has the advantages of safety, no toxicity, good water solubility, good stability and good activity of inhibiting or killing staphylococcus aureus in vitro, and can be used for preparing antibacterial drugs for inhibiting or killing staphylococcus aureus. 2. In the complementation of the disruption of bacterial cell membrane lipid structure based on vitamin a with the ability of the antimicrobial peptide membrane to disrupt, both produce a "membrane-disruption-sterilization" cascade synergistic effect. Under the pre