CN-121991194-A - Transcription factor CvbZIP81 related to selenium accumulation of cardamine violifolia and application thereof

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to a transcription factor CvbZIP81 related to selenium accumulation of cardamine violifolia (CARDAMINE VIOLIFOLIA) and application thereof. Based on the genome data of the cardamine violifolia, the invention carries out bioinformatics analysis such as identification of the whole genome level, chromosome location, phylogenetic analysis, genetic structure analysis, conserved domain identification and the like on 94 cardamine violifolia bZIP family members. Meanwhile, in combination with transcriptome data and gene expression pattern analysis, it was found that transcription factor CvbZIP81 was involved in regulating selenium uptake by up-regulating multiple selenium transport proteins (SULTR 1;2.1, SULTR4;2, SULTR4;1, SULTR3;4 and SULTR1; 2.2) affecting selenium accumulation by cardamine violae. The research result enriches the research content of bZIP transcription factors in a selenium absorption regulation network, and provides a target gene for creating a high-selenium accumulation type cardamine halinensis variety by using a biotechnology means.

Inventors

• XU FENG
• FENG LUXIN
• ZHENG JIARUI
• Zeng Sirui
• WANG SISI
• LI GE
• ZHANG WEIWEI
• YE JIABAO
• YANG WEI

Assignees

• 长江大学

Dates

Publication Date

20260508

Application Date

20260310

Claims (10)

1. 1. A transcription factor CvbZIP81 associated with selenium accumulation in cardamine halinensis, wherein said transcription factor CvbZIP81 is a member of the family of cardamine halinensis bZIP transcription factors that promote selenium absorption and accumulation by cardamine halinensis by activating sulfate transporter expression.
2. 2. The transcription factor CvbZIP81 of claim 1 wherein the nucleotide sequence of the gene encoded by the transcription factor CvbZIP81 is set forth in SEQ ID No. 1.
3. 3. The transcription factor CvbZIP81 of claim 1 wherein the expression level of the transcription factor CvbZIP81 is positively correlated with total selenium content in cardamine violifolia tissue.
4. 4. The transcription factor CvbZIP81 of claim 1 wherein the sulfate transporter is selected from one or more of SULTR, 2.1, SULTR4, 2, SULTR4, 1, SULTR3, 4 and SULTR1, 2.2.
5. 5. A polynucleotide encoding the transcription factor CvbZIP81 according to any one of claims 1 to 4.
6. 6. A method of modulating selenium accumulation in cardamine halinensis comprising modulating the expression activity or expression level of the transcription factor CvbZIP a 81 of any one of claims 1-4 in cardamine halinensis.
7. 7. The method of claim 6, wherein uptake and accumulation of selenium by cardamine halinensis is promoted by increasing the expression activity or expression level of the transcription factor CvbZIP.
8. 8. The method of claim 6, wherein the expression level is increased by introducing or overexpressing a polynucleotide encoding the transcription factor CvbZIP to cardamine cordierite.
9. 9. Use of a transcription factor CvbZIP81 according to any one of claims 1 to 4 or a polynucleotide according to claim 5 for regulating selenium accumulation in a plant.
10. 10. The use according to claim 9, wherein the plant is cardamine halinensis.

Description

Transcription factor CvbZIP81 related to selenium accumulation of cardamine violifolia and application thereof Technical Field The invention belongs to the technical field of genetic engineering, and particularly relates to a transcription factor CvbZIP81 related to selenium accumulation of cardamine violifolia (CARDAMINE VIOLIFOLIA) and application thereof. Background The cardamine violifolia (CARDAMINE VIOLIFOLIA) is an important perennial edible leaf vegetable of the cardamine genus of the cruciferae family, and is widely distributed in selenium resource rich areas such as Hubei enroshi and the like in China. The plant not only has good edible value, but also is rich in various nutritional ingredients, wherein the contents of protein, vitamin C and mineral substances are particularly remarkable. As a typical super selenium-accumulating plant, cardamine violifolia has received extensive attention in the academia. Researches show that under the condition of artificial cultivation, the selenium accumulation amount of the plants can reach 9741.05 mg/kg, the organic selenium ratio reaches 85.11%, and selenocysteine is the main material. With excellent superselenium gathering capability, cardamine violifolia is considered as a potential high quality safe dietary selenium supplement. The healthy vegetables serving as scientific selenium supplementing can supplement essential trace element selenium for human bodies, thereby maintaining the health of the human bodies. Therefore, the plant has important application potential in the fields of human nutrition and health no matter the plant is directly used as a healthy vegetable for supplementing selenium or used as a high-quality raw material for extracting organic selenium. Selenium (Se) is a trace element necessary for human and animals, is a key enzyme core component such as glutathione peroxidase (glutathione peroxidase, GPx) and thioredoxin reductase (thioredoxin reductase, trxR), can play a key antioxidant function by scavenging active oxygen, reduces oxidative stress-mediated cell damage and delays aging process. In addition to antioxidant effects, selenium has irreplaceable effects in maintaining metabolic homeostasis of thyroid hormone, enhancing immunity, reducing risk of multiple cancers and protecting cardiovascular and cerebrovascular health. Proper supplementation is critical, but excessive intake needs to be avoided. However, selenium cannot be synthesized by itself in humans, and it is necessary to rely on plants to convert inorganic selenium in the soil into a bioavailable organic form and to deliver it to humans and animals via the food chain. As a typical super-poly selenium plant, the cardamine violifolia not only has extremely strong tolerance and enrichment capability to selenium, but also can store selenium in organic forms such as selenocysteine (selenocysteine, seCys), selenomethionine (selenomethionine, seMet) and the like, and the organic selenium form is high in bioavailability, can reduce the potential toxicity of inorganic selenium, and further highlights the advantage of the cardamine violifolia as a selenium processing and extracting raw material. Since the cardamine violifolia has unique selenium accumulation and metabolism characteristics, the cardamine violifolia becomes an ideal natural model for researching the selenium absorption, transportation, assimilation and tolerance mechanisms of plants. Currently, selenium uptake in plants is largely dependent on a variety of transporter families, including sulfate transporter (SULTRs), nitrate transporter (NRTs), phosphate transporter (PHTs), ABC transporter, and the like, involved in selenium accumulation and transport processes. Among them, plant selenium absorption mainly depends on sulfate transporter for transporting selenate. Phosphate transporters absorb selenites. Nitrate transporters can mediate the transport of organic selenium such as methylselenocysteine (MeSeCys). In addition, the ABC transporter family is involved in cellular compartmentalization of selenium and regulation of plant selenium tolerance. Studies using Arabidopsis thaliana and selenium super-accumulating plant plume She Sitan Liu (STANLEYA PINNATA) as model plants show that the absorption and metabolism mechanisms of selenate are clearer than selenite. Research shows that sodium selenate treatment can significantly up-regulate the expression level of a plurality of sulfate transporter genes (SULTR), suggesting that such genes may play a key role in the absorption and transport processes of the selenate by cardamine violifolia. However, current research focuses on the implementation mechanism of selenium absorption, and the research on the regulation mechanism of the selenium absorption process is still deficient. In particular, in cardamine cordierite, no related research report on regulation of selenium absorption by bZIP transcription factors is seen, and the field is still to be further explored. The bZIP