CN-121991197-A - Antigen LARP1 of cysticercus cellulosae and application thereof

Abstract

The invention discloses an antigen LARP1 of bovine cysticercus and application thereof, and belongs to the technical field of biochemistry. The invention aims to provide a detection method and a treatment and prevention method for cysticercosis of cattle. The invention provides an antigen of cysticercus cellulosae, and the amino acid sequence of the antigen of Niu Nang cercaria is shown as SEQ ID NO. 4. Provides new candidate antigens for developing novel vaccines and developing specific diagnostic reagents and the like.

Inventors

• LIU MINGYUAN
• CHEN GUOLIANG
• SUN MINGFEI
• YOU XIHUO
• CAI XUEPENG
• ZHANG ZHUANGZHI
• DING JING
• LIU XIAOLEI

Assignees

• 吉林大学

Dates

Publication Date

20260508

Application Date

20260407

Claims (10)

1. 1. The cysticercus cariae antigen is characterized in that the amino acid sequence of the Niu Nang cysticercus cariae antigen is shown as SEQ ID NO. 4.
2. 2. A gene encoding the antigen of claim 1.
3. 3. A recombinant vector comprising the gene of claim 2.
4. 4. A recombinant host cell comprising the gene of claim 2.
5. 5. A kit for detecting cysticercosis in cattle, comprising the antigen of claim 1.
6. 6. The kit according to claim 5, wherein the kit comprises a sample buffer solution, a PVC bottom plate, a sample pad, a nitrocellulose membrane and a water absorbing pad, wherein the sample buffer solution comprises 0.01M PBS, 10% sucrose, 1% BSA, 1.4% Tween-20, 0.2% fluorescent microsphere-labeled goat anti-bovine IgG antibody and 0.4% fluorescent microsphere-labeled bovine cercaria antigen, and the amino acid sequence of the Niu Nang cercaria antigen is shown as SEQ ID NO. 4.
7. 7. The kit of claim 6, wherein the coupling ratio of the fluorescent microspheres to the goat anti-bovine IgG antibodies is 1:2, the coupling ratio of the fluorescent microspheres to the bovine cysticercus antigens is 1:20, the detection lines coated on the nitrocellulose membrane are mouse anti-human IgG antibodies, the goat anti-bovine IgG antibodies are coated on the quality control lines, and the coating concentration is 5 mu L/mm.
8. 8. Use of the antigenic peptide of claim 1, the coding gene of claim 2, the recombinant vector of claim 3 or the recombinant host cell of claim 4 for the preparation of a kit for diagnosing or detecting cysticercus cellulosae or an anti-cysticercus cellulosae antibody.
9. 9. The application of the LARP1 protein in preparing a vaccine for resisting bovine cysticercosis is characterized in that the amino acid sequence of the LARP1 protein is shown as SEQ ID NO. 4.
10. 10. The application of a recombinant vector or a recombinant host cell containing a gene for encoding LARP1 protein in preparing a vaccine for resisting bovine cysticercosis is characterized in that the gene for encoding LARP protein is shown as SEQ ID NO. 3.

Description

Antigen LARP1 of cysticercus cellulosae and application thereof Technical Field The invention belongs to the technical field of biochemistry, and particularly relates to an antigen LARP1 of bovine cysticercus and application thereof. Background Niu Nang cercaria (cysticercus bovis) is a zoonotic parasite of the genus zona of the family zonaceae. Larvae are mainly parasitic in bovine animals (such as cattle, buffalo, yak, tumor cattle and the like) to cause cysticercosis of cattle (commonly known as bovine cysticercosis). When a human ingests or semi-ingests beef or its products infected with bovine cysticercus, the cysticercus develops in the small intestine and becomes infected with taeniasis. Cattle are taken as an intermediate host as a main infectious source for human infection of taenia solium, and vaccine prevention is the most ideal measure for controlling the epidemic of bovine cysticercosis in China. If the immunization prevention can be implemented on cattle, the control method is greatly simplified, the control process is quickened, and the spread of the bovine tapeworm between people and livestock is greatly reduced and controlled. However, the development of the bovine immune vaccine is not stopped at present, and the important reasons are that the protective immune mechanism is unknown and the screening of protective antigens is delayed. At present, bovine cysticercosis lacks effective immunological diagnosis and detection technology and effective preventive vaccine, so that the epidemic of the disease in the whole world still presents an overall rising trend. The related researches of the bovine cysticercosis epidemic, monitoring, diagnosis, inspection, vaccine and the like in China are seriously lacking or even blank. Niu Nang cercaria disease is a great threat to beef cattle cultivation and beef food safety, and at present, prevention and control of the beef cercaria disease still take comprehensive prevention and control measures such as drug expelling (praziquantel powder or tablet oral administration is used for treatment), health education (popular science work of people eating habits in popular areas advocates eating safe beef after high-temperature cooking, recommends no raw beef), toilet improvement (harmless treatment of human excrement) and the like. Along with the development of molecular biology technology, parasite immunology research is continuously developed, and the separation of antigens and molecular cloning are continuously broken through, so that the pre-slaughter and post-slaughter immunology diagnosis and detection become possible, and meanwhile, sufficient technical guarantee is provided for effective vaccine development. Based on the method, the applicant establishes a bovine cysticercus and hexagonus cDNA library, uses bovine positive serum after bovine cysticercus infection, adopts immunological multi-round screening technology to carry out second generation sequencing on single colony with strong reactivity to identify new antigens, and provides new candidate antigens for research and development of novel vaccines, specific diagnostic reagents and the like. Disclosure of Invention The invention aims to provide a detection method and a treatment and prevention method for cysticercosis of cattle. The invention provides an antigen of cysticercus cellulosae, and the amino acid sequence of the antigen of Niu Nang cercaria is shown as SEQ ID NO. 4. The present invention provides a gene encoding the antigen described above. The present invention provides a recombinant vector comprising the above gene. The present invention provides a recombinant host cell comprising the above-described gene. The invention provides a kit for detecting cysticercosis cellulosae of cattle, which contains the antigen. The kit further comprises a sample buffer solution, a PVC bottom plate, a sample pad, a nitrocellulose membrane and a water absorption pad, wherein the sample buffer solution comprises 0.01M PBS, 10% sucrose, 1% BSA, 1.4% Tween-20, 0.2% fluorescent microsphere marked rabbit IgG antibody and 0.4% fluorescent microsphere marked bovine cysticercus cellulosae antigen, and the amino acid sequence of the Niu Nang cercus antigens is shown as SEQ ID NO. 4. Further limited, the coupling ratio of the fluorescent microsphere and the goat anti-bovine IgG antibody is 1:2, the coupling ratio of the fluorescent microsphere and the bovine cysticercus antigen is 1:20, the detection line coated on the nitrocellulose membrane is the mouse bovine human IgG antibody, the rabbit IgG antibody is coated on the quality control line, and the coating concentration is 5 mu L/mm. The invention provides an application of the antigen peptide, the coding gene, the recombinant vector or the recombinant host cell in preparing a kit for diagnosing or detecting cysticercus cellulosae or anti-cysticercus cellulosae antibody The invention provides application of LARP1 protein in preparing a vaccine for resisting bovi