CN-121991198-A - Polypeptide and application thereof

Abstract

The invention discloses a polypeptide and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the polypeptide is shown as SEQ ID NO. 1. The preparation method of the polypeptide adopts the conventional chemical solid-phase synthesis or recombinant expression mode to prepare. The application of the polypeptide is the application in preparing a medicament for improving psoriasis. The polypeptide can obviously inhibit the inflammatory reaction of HaCaT cells under the induction of M5 in vitro, has good anti-inflammatory activity, and in vivo experiments show that the polypeptide can effectively improve the psoriasis-like symptoms of mice induced by imiquimod, obviously reduce the thickening degree of epidermis acantha layers of mice in a polypeptide treatment group, obviously reduce the number of T cells at skin damage positions and obviously reduce the infiltration degree, and the polypeptide can improve the histopathological characteristics of psoriasis by inhibiting the thickening of epidermis acantha layers and reducing T cell infiltration. In addition, the polypeptide provided by the invention has good safety and application potential, and provides a new candidate molecule for clinical treatment of psoriasis and research and development of novel medicines.

Inventors

• YANG XINWANG
• WANG YING
• WANG TONG
• Yin Saige
• YANG MEIFENG
• LIU CHENGXING
• LUO MINGYING
• CHEN QINGFENG

Assignees

• 昆明医科大学

Dates

Publication Date

20260508

Application Date

20260407

Claims (4)

1. 1. A polypeptide is characterized in that the amino acid sequence of the polypeptide is shown as SEQ ID NO. 1.
2. 2. A nucleic acid, characterized in that, the nucleic acid encodes the polypeptide of claim 1.
3. 3. The method for preparing the polypeptide according to claim 1, wherein the polypeptide is prepared by conventional chemical solid phase synthesis or recombinant expression.
4. 4. Use of the polypeptide of claim 1 for the preparation of a medicament for ameliorating psoriasis.

Description

Polypeptide and application thereof Technical Field The invention belongs to the technical field of biological medicine, and particularly relates to a polypeptide and application thereof. Background Psoriasis is a chronic inflammatory skin disease, the clinical manifestations of the psoriasis are mainly erythema, scales and pruritus, and complications such as psoriasis arthritis, cardiovascular diseases and metabolic syndrome are often accompanied, so that the quality of life of a patient is influenced, and long-term health risks are brought. The current clinical psoriasis treatment can select external corticosteroid, methotrexate as a systemic drug, a biological agent targeting TNF-alpha/IL-17/IL-23 or a Tyk2 inhibitor, and the like according to the severity of the disease. However, the above-mentioned approaches have several limitations in long-term use, including corticosteroid induced skin atrophy, gastrointestinal intolerance caused by some drugs, hidden infection risk in the biologic injection route, attenuation of therapeutic effect of some patients due to drug resistance and generation of anti-drug antibodies, related risk of skin cancer due to phototherapy, and teratogenicity risk due to avermectin. Therefore, the development of a novel drug with both high efficiency and safety is extremely necessary. The polypeptide molecules have unique advantages in a plurality of treatment fields such as metabolic regulation, anti-infection, antioxidation, neuroprotection and the like by virtue of low molecular weight, high targeting specificity, stronger biological activity and lower toxic and side effects. Based on this, the present invention aims to provide a polypeptide for improving psoriasis to provide a new candidate molecule for clinical treatment of psoriasis. Disclosure of Invention A first object of the present invention is to provide a polypeptide for ameliorating psoriasis, and a second object of the present invention is to provide the use of the polypeptide. The first object of the present invention is achieved by a polypeptide having the amino acid sequence shown in SEQ ID NO. 1. The preparation method of the polypeptide adopts a conventional chemical solid-phase synthesis or recombinant expression mode to prepare the polypeptide. The second object of the invention is achieved in that the polypeptide is applied to the preparation of a medicament for improving psoriasis. The beneficial effects of the invention are as follows: 1. the polypeptide can obviously inhibit the inflammatory reaction of HaCaT cells under the induction of M5 in vitro, and has good anti-inflammatory activity; 2. in vivo experiments show that the polypeptide can effectively improve the psoriasis-like symptoms of mice induced by imiquimod, the thickening degree of epidermis acantha layers of mice treated by the polypeptide is obviously reduced, the number of T cells at skin damage parts is obviously reduced, and the infiltration degree is obviously reduced, so that the polypeptide can improve the histopathological characteristics of psoriasis by inhibiting the thickening of epidermis acantha layers and reducing T cell infiltration. 3. The polypeptide of the invention is derived from rana grahami (Odorrana graminea), is an active polypeptide of natural sources, has good safety and application potential, and provides a new candidate molecule for clinical treatment of psoriasis and research and development of novel medicines. Drawings FIG. 1 shows the results of inhibition of expression levels of TNF- α mRNA in HaCaT cells induced by M5 by a polypeptide of the invention of example 1; FIG. 2 is a graph showing the improvement effect of the polypeptide of the invention on the skin lesions of BALB/c mice induced by imiquimod in example 2, wherein A is a graph showing the appearance of the skin lesions of each group of mice, and B is a statistical graph showing the PASI scores of each group of mice; FIG. 3 is a graph showing the results of the effect of the polypeptide of the present invention on the skin histopathological characteristics and T cell infiltration rate of mice in psoriasis model example 2, wherein A is the HE staining result of the back skin tissue of each group of mice, B is the quantification result of the thickness of the back skin spine layer of each group of mice, C is the immunohistochemical staining result of CD3 + T cells in the back skin tissue of each group of mice, and D is the quantification result of the infiltration rate of CD3 + T cells in the back skin tissue of each group of mice. Detailed Description The invention is described in further detail below with reference to the drawings and examples, but is not limited in any way to any changes or modifications made based on the teachings of the invention, which fall within the scope of the invention. The invention provides a polypeptide, the amino acid sequence of which is FLPGPLRVAAKVRRAVFCEIFKRC as shown in SEQ ID NO. 1. The polypeptide is identified fro