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CN-121991257-A - Mulberry leaf polysaccharide, preparation method and application thereof in treating AFB 1-induced liver injury of mice

CN121991257ACN 121991257 ACN121991257 ACN 121991257ACN-121991257-A

Abstract

The invention relates to the technical field of medicine application, and discloses mulberry leaf polysaccharide, a preparation method and application thereof in treating AFB 1-induced liver injury of mice. The mulberry leaf polysaccharide can be used alone or in combination with other Chinese medicinal extracts to prepare pharmaceutical composition. Experimental results show that the liver injury symptoms of mice treated by the mulberry leaf polysaccharide are obviously improved, the trend of liver index drop is relieved, and the liver lesion degree is obviously reduced. Therefore, the mulberry leaf polysaccharide provided by the invention can effectively treat liver injury and concomitant oxidative stress caused by AFB1, and has a wide application prospect in the research field of mulberry leaf polysaccharide antioxidant preparations. This finding not only provides a powerful support for the subsequent development of the polysaccharide, but also provides new ideas and directions for further optimization and innovation.

Inventors

  • SI HONGBIN
  • Kuang Tiantian
  • XIE ZONGGU
  • SHI XINRU
  • ZHANG YUAN
  • LU JUNZHI
  • LI YEHONG
  • SUN XIAOMENG

Assignees

  • 广西大学

Dates

Publication Date
20260508
Application Date
20241107

Claims (10)

  1. 1. A preparation method of mulberry leaf polysaccharide is characterized by comprising the following steps: (1) Picking fresh mulberry leaves, drying in a baking oven, soaking in absolute ethyl alcohol for decolorization and degreasing treatment, and drying in the baking oven again for standby; (2) Taking the mulberry leaf raw material dried in the step (1), adding water, boiling, maintaining the boiling state for a period of time, filtering to obtain a primary extract, repeating the steps for a plurality of times, concentrating the primary extract by using a rotary evaporator, and centrifuging to remove residue sediment at the lower layer to obtain the extract; (3) Adding reagent into the extractive solution, stirring, centrifuging, collecting upper liquid, adding reagent again, repeating the above steps until no protein appears in the middle, collecting upper liquid, precipitating with ethanol, collecting alcohol precipitate the next day, and processing with freeze dryer to obtain Sang Shecu polysaccharide; (4) Taking crude mulberry leaf polysaccharide to prepare polysaccharide liquid, adding resin, oscillating, centrifuging, collecting supernatant, adopting an ethanol system for alcohol precipitation, and freeze-drying to obtain the mulberry leaf polysaccharide.
  2. 2. A method for preparing mulberry leaf polysaccharide according to claim 1, wherein the oven drying temperature in the step (1) is 50-65 ℃.
  3. 3. A method for preparing mulberry leaf polysaccharide according to claim 1, wherein the mass-to-volume ratio of the mulberry leaf raw material to deionized water added in the step (2) is 1:20-1:40, and the boiling time is maintained for 2.5-3.5h.
  4. 4. The method for preparing mulberry leaf polysaccharide according to claim 1, wherein the reagent in the step (3) is a Sevage reagent, and the ratio of n-butanol to chloroform in the Sevage reagent is 1:4.
  5. 5. The method for preparing mulberry leaf polysaccharide according to claim 1, wherein the ethanol system in the step (3) and the step (4) is 75% -85%.
  6. 6. A method for preparing mulberry leaf polysaccharide according to claim 1, wherein the resin in the step (4) is AB-8 macroporous resin.
  7. 7. The method for preparing mulberry leaf polysaccharide according to claim 1, wherein the shaking time in the step (4) is 11-13h, the centrifugation time is 10-15min, and the rotation speed is 3800-4500r/min.
  8. 8. A mulberry leaf polysaccharide prepared according to the method of any one of claims 1 to 7.
  9. 9. Use of a mulberry leaf polysaccharide according to claim 8 in an in vitro antioxidant capacity.
  10. 10. Use of a mulberry leaf polysaccharide according to claim 8 in the treatment of AFB 1-induced liver injury in mice.

Description

Mulberry leaf polysaccharide, preparation method and application thereof in treating AFB 1-induced liver injury of mice [ Field of technology ] The invention relates to the technical field of medicine application, in particular to mulberry leaf polysaccharide, a preparation method and application thereof in treating AFB 1-induced liver injury of mice. [ Background Art ] Sang Sheshu Moraceae plants are mainly distributed in Asia, europe, north America and other areas. China is the largest mulberry planting country in the world and has a mulberry planting history of over 4000 years. The mulberry leaf is a dry leaf of plant mulberry, also called iron leaf and silkworm leaf. Modern researches show that the mulberry leaves contain various natural active ingredients such as flavone, alkaloid, polysaccharide, polyphenol, organic acid and the like, play an important role in anti-inflammatory, obesity treatment, blood sugar reduction, arteriosclerosis resistance, oxidation resistance and the like, and are widely applied to food additives and biomedical industries. Aflatoxin B1 (AFB 1) is a mycotoxin, has strong toxicity and carcinogenicity, and has adverse effects on human and animal health. According to the reports of the grain and agricultural organization, about 25% of the crops in the world are contaminated with mycotoxins. AFB1 is widely used in livestock feed and human food, and causes huge economic loss to agriculture and animal husbandry. Chronic low dose exposure to AFB1 can lead to chronic toxicity and even liver cancer, AFB1 has been shown to trigger liver tissue damage and liver function damage. According to incomplete statistics, under the influence of high-temperature and high-humidity weather in Yangtze river basin and south China, the grain loss caused by fungi (mainly aflatoxin, zearalenone, vomitoxin and the like) pollution in China is up to 3100 ten thousand tons each year, and the economic loss is up to 680-850 hundred million yuan. Although researches report that mulberry leaves and extracts thereof have potential liver protection effects, the mulberry leaves and extracts thereof have a certain liver protection effect on liver injury caused by various chemical poisons (including alcohol, acetaminophen, carbon tetrachloride and the like), the specific action mechanism and the functional components of the mulberry leaves are not fully researched, and the research on the mulberry leaf polysaccharide (Mulberryleafpolysaccharides, MLP) for interfering with the AFB 1-induced liver injury is not reported in domestic and foreign documents at present. Therefore, the invention aims to provide an application of mulberry leaf polysaccharide in treating AFB1 induced liver injury of mice. The foregoing background is only for the purpose of providing an understanding of the inventive concepts and technical aspects of the present application and is not necessarily prior art to the present application and is not intended to be used as an aid in the evaluation of the novelty and creativity of the present application in the event that no clear evidence indicates that such is already disclosed at the date of filing of the present application. [ Invention ] In order to solve the problems of liver tissue injury and liver function injury triggered by AFB1 in the background technology, the invention provides application of mulberry leaf polysaccharide in treating AFB 1-induced liver injury of mice. The mulberry leaf polysaccharide can be used as a medicinal active ingredient to prepare various clinical medicinal preparations, or is a medicinal composition prepared by the mulberry leaf polysaccharide and other traditional Chinese medicines or traditional Chinese medicine extracts or traditional Chinese medicine effective parts or chemical components or auxiliary materials together, and is used for treating induced liver injury of AFB 1. In order to achieve the above purpose, the present invention adopts the following technical scheme: A preparation method of mulberry leaf polysaccharide comprises the following steps: (1) Picking fresh mulberry leaves, drying in a baking oven, soaking in absolute ethyl alcohol for decolorization and degreasing treatment, and drying in the baking oven again for standby; (2) Taking the mulberry leaf raw material dried in the step (1), adding water, boiling, maintaining the boiling state for a period of time, filtering to obtain a primary extract, repeating the steps for a plurality of times, concentrating the primary extract by using a rotary evaporator, and centrifuging to remove residue sediment at the lower layer to obtain the extract; (3) Adding reagent into the extractive solution, stirring, centrifuging, collecting upper liquid, adding reagent again, repeating the above steps until no protein appears in the middle, collecting upper liquid, precipitating with ethanol, collecting alcohol precipitate the next day, and processing with freeze dryer to obtain Sang Shecu polysaccharide; (4)