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CN-121991545-A - Tannic acid-assisted anti-fouling coating for seawater system, and preparation method and application thereof

CN121991545ACN 121991545 ACN121991545 ACN 121991545ACN-121991545-A

Abstract

The invention discloses a tannic acid-assisted anti-fouling coating for an internal seawater system, and a preparation method and application thereof. The invention uses the adsorption of tannic acid as the anchoring part of the anti-fouling coating, plays a good fixing role on the surface of the substrate, and forms a physical interval between tannic acid and the active center of protease through the connection of the hydrophilic substance in the middle layer, thereby reducing the possible conformational change or active site blocking of the tannic acid to the protease, better maintaining the protease activity and simultaneously providing enough sites for the protease, so that the protease can be effectively fixed on the surface of the substrate to form the anti-fouling coating. The protease on the surface of the antifouling coating can effectively degrade protein components in the biofilm matrix, thereby preventing adhesion of bacteria and algae and having excellent antibacterial and anti-algae performances. The preparation method is simple, convenient, quick and low in cost, and is suitable for preparing marine equipment and facility protective coatings in special scenes.

Inventors

  • MA CHUNFENG
  • XIE QINGYI
  • Dou Bohao
  • PAN JIANSEN
  • ZHANG GUANGZHAO

Assignees

  • 华南理工大学

Dates

Publication Date
20260508
Application Date
20260205

Claims (10)

  1. 1. A preparation method of a tannic acid-assisted anti-fouling coating of an internal seawater system is characterized by comprising the following steps of, (1) The preparation of the tannic acid modified coating comprises the steps of immersing a pretreated substrate in tannic acid solution with the mass concentration of 1-10 mg/mL, incubating, washing and drying the surface of the substrate to obtain the tannic acid modified coating; (2) The preparation of the hybridized hydrophilic modified coating comprises the steps of soaking the tannic acid modified coating obtained in the step (1) in hydrophilic active substance solution with the mass concentration of 1-10 mg/mL, incubating, washing and surface blow-drying to obtain the hybridized hydrophilic modified coating, wherein the hydrophilic active substance is at least one of polyethyleneimine, polyvinyl alcohol, polyethylene glycol and aspartic acid; (3) The preparation of the tannic acid-assisted anti-fouling coating of the internal seawater system comprises the steps of activating the hybridized hydrophilic modified coating in the step (2) by using an activator solution with the mass concentration of 0.01-0.1 mg/mL, and then soaking the activated hydrophilic modified coating in a protease solution with the mass concentration of 1-10 mg/mL to fix protease on the surface of a substrate to obtain the tannic acid-assisted anti-fouling coating of the internal seawater system, wherein the activator is at least one of glutaraldehyde, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin, and the protease is at least one of lysozyme, alkaline protease, trypsin, pepsin, proteinase K and subtilisin.
  2. 2. The method for preparing the tannic acid-assisted seawater system antifouling coating according to claim 1, wherein the substrate in the step (1) is a metal substrate or a non-metal substrate, the metal substrate is one of a titanium alloy, a copper alloy or a steel plate, and the non-metal substrate is one of glass, ceramic, polytetrafluoroethylene or nylon.
  3. 3. The method for preparing the tannic acid-assisted seawater system antifouling coating according to claim 1, wherein the incubation temperature in the step (1), the step (2) and the step (3) is 20-30 ℃, and the incubation time is 0.5-4 h.
  4. 4. The method for producing a tannic acid-assisted seawater system antifouling coating according to claim 1, wherein the protease activity in the step (3) is 12.6U/mg.
  5. 5. The method for preparing the tannic acid-assisted seawater system antifouling coating according to claim 1, wherein the tannic acid solution in the step (1) is prepared by dissolving tannic acid into a solvent and uniformly stirring, the hydrophilic active substance solution in the step (2) is prepared by dissolving hydrophilic active substances into a solvent and uniformly stirring, the protease solution in the step (3) is prepared by dissolving protease into a solvent and uniformly stirring, and the activator solution is prepared by dissolving activator into a solvent and uniformly stirring.
  6. 6. The method for preparing an anti-fouling coating of a tannic acid-assisted seawater system of claim 5, wherein the solvent is at least one of deionized water, artificial seawater, tris-HCl solution, and PBS buffer.
  7. 7. A tannic acid-assisted anti-fouling coating for a seawater system, characterized in that it is prepared according to the preparation method of any one of claims 1 to 6.
  8. 8. The tannic acid-assisted seawater system antifouling coating of claim 7, wherein the tannic acid-assisted seawater system antifouling coating has an adsorption capacity of 2000-4500 Hz.
  9. 9. The tannic acid-assisted seawater system antifouling coating of claim 7, wherein the tannic acid-assisted seawater system antifouling coating has a water contact angle of 31-34 °.
  10. 10. Use of a tannic acid-assisted anti-fouling coating of an inland seawater system according to any of claims 7 to 9 for anti-fouling of profiled and small components in marine equipment and facilities in real sea environments.

Description

Tannic acid-assisted anti-fouling coating for seawater system, and preparation method and application thereof Technical Field The invention belongs to the field of antifouling materials, and particularly relates to a tannic acid-assisted seawater system antifouling coating, and a preparation method and application thereof. Background Marine fouling organisms refer to biofouling formed by the adsorption, growth and propagation of marine microorganisms, animals and plants on the surfaces of marine equipment and facilities. The attachment and growth of these organisms severely affect the safe and long-lasting operation of marine equipment. Specifically, the attachment of fouling organisms can add the dead weight and roughness of the ship, increase the sailing resistance, reduce the sailing speed and maneuverability, induce the metal corrosion phenomena of devices such as offshore drilling platforms, wave energy power generation platforms and the like to bring potential safety hazards, and block the meshes of a cultivation box to cause death of economic fishes due to hypoxia or environmental deterioration. In the attachment of biofouling organisms, contact between the organism and the surface of the material is the primary stage of forming the fouling. Thus, the means for preventing fouling in the initial stages of fouling includes preventing the fouling from contacting the surface of the material or killing the fouling that has adhered to the surface of the material. Currently, the coating method is the most widespread and effective anti-fouling technique, in which a metal or organic anti-fouling agent is mixed with a resin to form an anti-fouling paint, which is then fixed to equipment and facility surfaces by a primer and a tie-coat. Although the antifouling effect is excellent, the metal or organic antifouling agent is inevitably released into seawater to destroy the ecological environment of the ocean, and affect the growth and reproduction of non-target organisms, so the development of green environment-friendly paint is urgent. On the other hand, different application environments bring certain challenges to the coating of the coating, such as in a ship sea-going system with serpentine and narrow, the coating construction difficulty is high due to the narrow inner wall of a pipeline, the complex system and the large number of special-shaped pieces, and the anti-fouling coating is usually effective for no more than 5 years, and cannot be recoated in a pipe, so that the maintenance period of a ship pipeline system cannot be matched. Therefore, there is a need to develop an environment-friendly antifouling coating which has a bactericidal effect and is easy to apply, so that the antifouling coating can be applied to special components such as a marine pipeline sea-going system and the like, and has an antifouling effect. Disclosure of Invention Aiming at the defects of the prior anti-fouling coating technology, the primary aim of the invention is to provide a tannic acid-assisted anti-fouling coating for an internal seawater system, and a preparation method and application thereof. The preparation method is simple to operate and low in cost, is suitable for coating of the antifouling coating on the surfaces of various base materials in various scenes, has universality, fully exerts the special and efficient characteristics of protease, effectively kills fouling organisms and prevents the adhesion of the fouling organisms, and the protease and the film-forming material are both from nature, so that the product after degradation is green and environment-friendly and does not pollute the environment. The second object of the invention is to provide a tannic acid-assisted anti-fouling coating for an internal seawater system prepared by the preparation method. A third object of the present invention is to provide the use of the tannic acid-assisted anti-fouling coating of an inland seawater system. The primary aim of the invention can be achieved by the following technical scheme: A method for preparing an anti-fouling coating of a tannic acid-assisted seawater system comprises the following steps, (1) The preparation of the tannic acid modified coating comprises the steps of immersing a pretreated substrate in tannic acid solution with the mass concentration of 1-10 mg/mL, incubating, washing and drying the surface of the substrate to obtain the tannic acid modified coating; (2) The preparation of the hybridized hydrophilic modified coating comprises the steps of soaking the tannic acid modified coating obtained in the step (1) in hydrophilic active substance solution with the mass concentration of 1-10 mg/mL, incubating, washing and surface blow-drying to obtain the hybridized hydrophilic modified coating, wherein the hydrophilic active substance is at least one of polyethyleneimine, polyvinyl alcohol, polyethylene glycol and aspartic acid; (3) The preparation of the tannic acid-assisted anti-fouling coating o