CN-121991754-A - Giant salamander oil extraction and microcapsule preparation method and application thereof in antioxidant products

Abstract

The invention discloses a giant salamander oil extraction and microcapsule preparation method and application thereof in antioxidant products, comprising the following steps: pretreating giant salamander adipose tissue, tea polyphenol cellulose inclusion compound, citric acid and EDTA-2Na, adding protease for enzymolysis, adding phytic acid for degumming, and inactivating enzyme and separating to obtain the giant salamander oil. Preparing giant salamander oil into liposome, mixing with wall material solution, granulating to obtain microcapsule. The invention realizes the high-efficiency extraction of the giant salamander oil and the comprehensive improvement of the oxidation resistance and the water dispersion property of the giant salamander oil microcapsule, and is suitable for the development of the giant salamander oil oxidation resistance product.

Inventors

• WANG XIAOYU
• HE JIAJIA
• LUO QINGHUA
• HE JING
• XU BIXIANG
• WANG ZHIQIANG
• ZENG QI
• Xia Shaoxuan
• LI LAN
• CHEN XINGYI

Assignees

• 湖南天劲制药有限责任公司
• 长沙学院

Dates

Publication Date

20260508

Application Date

20260409

Claims (10)

1. 1. The giant salamander oil extraction method is characterized by comprising the following steps of: pretreating giant salamander adipose tissue, tea polyphenol cellulose inclusion compound, citric acid and EDTA-2Na, adding protease for enzymolysis, adding phytic acid for degumming, and inactivating enzyme and separating to obtain the giant salamander oil.
2. 2. The method for extracting giant salamander oil according to claim 1, wherein the tea polyphenol cellulose clathrate is obtained by mixing modified microcrystalline cellulose with tea polyphenol and then crosslinking the mixture with chitosan quaternary ammonium salt and sodium tripolyphosphate.
3. 3. The method for extracting giant salamander oil according to claim 2, characterized in that the modified microcrystalline cellulose is obtained by modifying microcrystalline cellulose with at least one of oxygen, enzymatic modification and phosphoric acid modification; and/or, the tea polyphenol cellulose inclusion compound also comprises polyethylene glycol; and/or the mass ratio of the giant salamander adipose tissue to the tea polyphenol cellulose inclusion compound is 100:5-10; and/or the mass ratio of the giant salamander adipose tissue to the citric acid is 1000:2-4; and/or the mass ratio of the giant salamander adipose tissue to EDTA-2Na is 1000:0.3-0.7; and/or the temperature of the pretreatment is 40-50 ℃.
4. 4. The method for extracting giant salamander oil according to claim 1, wherein said protease is at least one selected from the group consisting of complex protease and papain; And/or the mass ratio of the giant salamander adipose tissue to the protease is 1000:10-15; and/or the mass ratio of the giant salamander adipose tissue to the phytic acid is 1000:2-3; and/or the enzymolysis temperature of the protease is 40-60 ℃; And/or the enzymolysis time of the protease is 1-4 hours.
5. 5. The microcapsule preparation method is characterized in that giant salamander oil obtained by the extraction method according to any one of claims 1-4 is used as a raw material.
6. 6. The method for preparing microcapsules according to claim 5, comprising preparing liposome from giant salamander oil, mixing with wall material solution, and granulating to obtain microcapsules.
7. 7. The method of preparing microcapsules of claim 6, wherein the liposomes further comprise soy lecithin and cholesterol; and/or, the liposome further comprises at least one of vitamin E and tea polyphenol; and/or the wall material solution comprises maltodextrin, sodium starch octenyl succinate and carboxymethyl chitosan.
8. 8. The method of preparing microcapsules according to claim 7, comprising the steps of: mixing soybean lecithin, cholesterol and PBS buffer solution, and adding the giant salamander oil for ultrasonic dispersion to obtain giant salamander oil liposome; And mixing and homogenizing the giant salamander lipidosome with the wall material solution, and drying to obtain the microcapsule.
9. 9. The method for preparing the microcapsule according to claim 7, wherein the mass ratio of giant salamander oil to soybean lecithin to cholesterol in the liposome is 10:20-25:3-5; And/or the mass ratio of maltodextrin to sodium starch octenyl succinate to carboxymethyl chitosan in the wall material solution is 2-3:1-2:1.
10. 10. The application of giant salamander oil obtained by the extraction method according to any one of claims 1-4 or the microcapsule obtained by the preparation method according to any one of claims 5-9 in antioxidant products.

Description

Giant salamander oil extraction and microcapsule preparation method and application thereof in antioxidant products Technical Field The invention belongs to the technical field of foods and medicines, and particularly relates to a giant salamander oil extraction and microcapsule preparation method and application thereof in antioxidant products. Background The content of the fat tissue at the tail of the giant salamander is rich and is about 5-8% of the weight, so that the giant salamander is an important raw material for extracting functional grease. Research shows that giant salamander oil is rich in various unsaturated fatty acids, and has obvious physiological functions of resisting inflammation, regulating blood fat, promoting brain development and the like. The giant salamander oil can be extracted by adopting a water enzyme method, and the principle is that protein membranes formed by combining phospholipid and protein outside fat cells are destroyed by the hydrolysis of protease on the protein, so that the grease can be released. Compared with the traditional methods of high-temperature steaming, squeezing, solvent extraction and the like, the aqueous enzymatic method has the advantages of mild conditions, capability of effectively protecting the functional components in the grease from being damaged by high temperature, high oil extraction rate, no organic solvent residue, simple process operation, high production safety and the like. However, the conventional aqueous enzymatic method lacks an effective pretreatment means, and cells are not sufficiently broken, so that the enzymolysis efficiency is limited and the extraction time is long. And the oil-water emulsion obtained after enzymolysis has higher stability, difficult demulsification, low oil separation efficiency and high extraction loss rate because the oil is easy to remain in a water phase and a solid phase. In addition, the grease has large contact area with air in the enzymolysis process, is easy to generate oxidative deterioration, and finally leads to poor oxidation resistance of the extracted giant salamander oil, thereby limiting the application of the giant salamander oil. Disclosure of Invention The invention aims to provide a giant salamander oil extraction and microcapsule preparation method and application thereof in an antioxidant product, so as to solve at least one aspect of the problems and defects in the prior art. In order to achieve the above purpose, the present invention provides the following technical solutions: The giant salamander oil extraction method comprises the following steps: pretreating giant salamander adipose tissue, tea polyphenol cellulose inclusion compound, citric acid and EDTA-2Na, adding protease for enzymolysis, adding phytic acid for degumming, and inactivating enzyme and separating to obtain the giant salamander oil. As a further improvement of the invention, the tea polyphenol cellulose inclusion compound is obtained by mixing modified microcrystalline cellulose with tea polyphenol and then crosslinking by chitosan quaternary ammonium salt and sodium tripolyphosphate. As a further improvement of the present invention, the modified microcrystalline cellulose is obtained by modifying microcrystalline cellulose with at least one of oxygen, enzymatic modification, and phosphoric acid modification. As a further improvement of the invention, the modified microcrystalline cellulose is obtained by modifying microcrystalline cellulose by treating the microcrystalline cellulose with oxygen plasma at 30-50W for 1-10 min. As a further improvement of the present invention, the modified microcrystalline cellulose is obtained by enzymatic modification of microcrystalline cellulose with cellulase. As a further improvement of the invention, the dosage of the cellulase is 1-3% of the mass of the microcrystalline cellulose. As a further improvement of the invention, the enzymolysis temperature of the cellulase is 40-60 ℃. As a further improvement of the invention, the enzymolysis pH of the cellulase is 4.0-6.0, preferably 4.5-5.0. As a further improvement of the invention, the modified microcrystalline cellulose is obtained by soaking and modifying microcrystalline cellulose with a phosphoric acid solution with the mass concentration of 0.2-0.7%, and the mass concentration of the phosphoric acid solution is preferably 0.4-0.6%. As a further improvement of the present invention, the modified microcrystalline cellulose has a particle size of 10 to 40. Mu.m, preferably 20 to 30. Mu.m. As a further improvement of the invention, the tea polyphenol cellulose clathrate is prepared by the following method: dispersing the modified microcrystalline cellulose and tea polyphenol in an ethanol water solution, stirring, filtering and drying to obtain tea polyphenol loaded cellulose; mixing the tea polyphenol loaded cellulose with chitosan quaternary ammonium salt, and adding sodium tripolyphosphate for crosslinking to obtain the