CN-121991782-A - Micro-gravity environment microalgae closed culture container, micro-gravity environment microalgae closed culture system and application method of micro-gravity environment microalgae closed culture container

CN121991782ACN 121991782 ACN121991782 ACN 121991782ACN-121991782-A

Abstract

The invention provides a micro-gravity environment microalgae closed culture container, a micro-gravity environment microalgae closed culture system and a use method thereof, wherein the culture container comprises the following components; the culture solution box comprises a wavy solution box top wall and a wavy solution box bottom wall which are made of hydrophobic and breathable materials, a solution box side wall which is connected with the solution box top wall and the solution box bottom wall in a matched mode, and a light source component which penetrates through the inside of the culture solution box, wherein the upper half shell is matched with the solution box top wall to form an upper air chamber, the lower half shell is matched with the solution box bottom wall to form a lower air chamber, and an inlet and an outlet are respectively arranged on the culture solution box, the upper air chamber and the lower air chamber. The micro-gravity environment microalgae closed culture container provided by the invention increases the contact area of the culture solution box and the air, improves the exchange efficiency of the microalgae culture solution and oxygen and carbon dioxide in the air, and in addition, the whole reactor is composed of fixed parts, so that the structure of the culture device is simplified, and the manufacturing cost is reduced.

Inventors

MAO RUIXIN
LIU LITAO
WU HAO
ZHANG ZHEN
ZHANG LIANGCHANG
FAN HAONAN
ZHOU GUODONG

Assignees

中国航天员科研训练中心

Dates

Publication Date: 20260508
Application Date: 20241101

Claims (10)

1. The micro-gravity environment microalgae closed culture container is characterized by comprising a culture solution box (1.1), an upper half shell (1.2) and a lower half shell (1.3) which are respectively connected to the upper end and the lower end of the culture solution box (1.1); The culture solution box (1.1) comprises a wave-shaped solution box top wall (1.12) and a solution box bottom wall (1.13) which are made of hydrophobic and breathable materials, a solution box side wall (1.11) which is connected with the solution box top wall (1.12) and the solution box bottom wall (1.13) in a matching way, and a light source assembly (1.14) which is arranged in the culture solution box (1.1) in a penetrating way; the upper half shell (1.2) is matched with the top wall (1.12) of the liquid tank to form an upper air chamber (1.21), and the lower half shell (1.3) is matched with the bottom wall (1.13) of the liquid tank to form a lower air chamber (1.31); the culture solution box (1.1), the upper air chamber (1.21) and the lower air chamber (1.31) are respectively provided with an inlet and an outlet for connecting pipelines.
2. The micro-gravity environment microalgae closed culture container according to claim 1, wherein the side wall (1.11) of the liquid tank comprises a first wall plate (1.111) and a second wall plate (1.112) which are arranged in parallel, the upper edge and the lower edge of the first wall plate are wavy, a third wall plate (1.113) and a fourth wall plate (1.114) which are arranged perpendicular to the first wall plate (1.111), two ends of the third wall plate (1.113) are respectively connected with the left ends of the first wall plate (1.111) and the second wall plate (1.112), and two ends of the fourth wall plate (1.114) are respectively connected with the right ends of the first wall plate (1.111) and the second wall plate (1.112).
3. The micro-gravity environment microalgae closed culture container according to claim 2, wherein the light source assembly (1.14) comprises a transparent light source outer tube (1.141) penetrating through the first wall plate (1.111) and the second wall plate (1.112), and a light emitter (1.142) penetrating through the light source outer tube (1.141).
4. The micro-gravity environment microalgae closed culture container according to claim 3, wherein the illuminator (1.142) is an LED lamp strip composed of three LED lamps of red, white and blue with adjustable luminous intensity respectively.
5. The micro-gravity environment microalgae closed culture container according to claim 1, wherein a plurality of clamping components (1.15) are respectively connected to the peripheries of the upper half shell (1.2) and the lower half shell (1.3), sealing materials are coated or adhered to the upper edge and the lower edge of the side wall (1.11) of the liquid tank under the action of the corresponding clamping components (1.15), the clamping components (1.15) comprise flange components and buckle components, and sealing connection is formed between the upper half shell (1.2) and the top wall (1.12) of the liquid tank, between the side wall (1.11) of the liquid tank and the bottom wall (1.13) of the liquid tank, and between the bottom wall (1.13) of the liquid tank and the lower half shell (1.3) of the liquid tank.
6. The micro-gravity environment microalgae closed culture container as claimed in claim 1, wherein the hydrophobic and breathable materials for preparing the top wall (1.12) and the bottom wall (1.13) of the liquid tank are composed of a hydrophobic and breathable film and a protective net covered on the outer side of the hydrophobic and breathable film, and the protective net is made of transparent materials including polycarbonate and polyethylene terephthalate.
7. A micro-gravity environment microalgae closed culture system, which is characterized by comprising a plurality of micro-gravity environment microalgae closed culture containers (1) as claimed in any one of claims 1-6, a fan (2) communicated with an upper air chamber (1.21) and a lower air chamber (1.31) through pipelines, a water pump (3) communicated with a culture solution tank (1.1) through pipelines, and a controller (4) electrically connected with the light source assembly (1.14), the fan (2) and the water pump (3) respectively.
8. The micro-gravity environment microalgae closed culture system according to claim 7, further comprising air chamber sensors which are respectively arranged on the upper half shell (1.2) and the lower half shell (1.3) in a penetrating way and are electrically connected with the controller (4), wherein the air chamber sensors comprise a carbon dioxide sensor and an oxygen sensor.
9. The micro-gravity environment microalgae closed culture system according to claim 7, further comprising a liquid tank sensor penetrating through the side wall (1.11) of the liquid tank and electrically connected with the controller (4), wherein the liquid tank sensor comprises a pH value sensor and a dissolved oxygen sensor.
10. A method for using the micro-gravity environment microalgae closed culture system, which is characterized by being applied to the micro-gravity environment microalgae closed culture system according to any one of claims 7-9, and comprising the following steps: s1, injecting microalgae culture solution with the pH value of 8-9 into the culture solution box (1.1); s2, starting a fan (2), a water pump (3) and a light source assembly (1.14) in the micro-gravity environment microalgae closed culture system; S3, when the pH value of the microalgae culture solution is more than or equal to 10 or the concentration of dissolved oxygen is more than or equal to 20mg/L, weakening the illumination intensity and improving the speed of the water pump and the rotation speed of the fan, and when the pH value is less than 8, increasing the illumination intensity and reducing the speed of the water pump (3) and the rotation speed of the fan (2).

Description

Micro-gravity environment microalgae closed culture container, micro-gravity environment microalgae closed culture system and application method of micro-gravity environment microalgae closed culture container Technical Field The invention relates to the technical field of microalgae culture, in particular to a microgravity environment microalgae closed culture container, a microgravity environment microalgae closed culture system and a microgravity environment microalgae closed culture using method. Background The controlled ecological life support system (Control 1ed Ecological Life Support System, CELSS) is a closed loop ecological system for continuously regenerating life support materials such as food, oxygen, water and the like for passengers in a long-term sealed environment in particular environments such as manned deep space exploration or extraterrestrial space settlement. The microalgae has the characteristics of high photosynthetic efficiency, intensive culture space, rapid growth and propagation, high nutritive value, rich antioxidants and the like, and can be used as a biological component of CELSS to play important roles in the aspects of atmosphere balance regulation and control, food stability supply and the like. However, how to realize microalgae culture in a microgravity environment is always a technical problem. To realize continuous, stable and controllable culture of microalgae in a space microgravity environment, the problems of three-phase mass transfer and separation of algae cells, culture solution and atmosphere in the microgravity environment are faced. In the microalgae cultivation process, the transfer and supply of photosynthetic raw materials such as carbon dioxide to algae cells are required to be completed, and meanwhile, oxygen generated by photosynthesis is discharged out of a cultivation system. In ground application, gas phase raw material conveying and enhanced substance transfer are often completed in an excessive aeration mode, namely carbon dioxide gas firstly enters a liquid phase main body through excessive aeration dissolution and then is transferred into algae cells along with liquid phase flow diffusion, and redundant air supply and oxygen naturally overflow from the liquid phase under the action of gravity and buoyancy, so that gas exchange is realized. Under the condition of space microgravity, the method is not feasible, on one hand, the algae liquid is difficult to be directly aerated, on the other hand, excessive gas and generated oxygen cannot be discharged automatically, the homogeneity of the liquid phase can be destroyed, the gas adheres to the surface of the algae cells, and the transmission of substances among three phases is affected. In recent years, the liquid chamber membrane technology is widely applied, and can be used as a technical way for realizing closed culture of microalgae. In the three-phase mass transfer and separation process, carbon dioxide is conveyed to the liquid phase through the breathable film under the pushing of pressure (concentration) difference and utilized by algae cells, and generated oxygen also enters the atmosphere from the liquid phase through the breathable film under the pushing of pressure (concentration) difference. However, the existing micro-gravity environment microalgae closed culture container has the problems of low gas exchange efficiency, complex structure and high manufacturing cost. Disclosure of Invention In order to solve the problems of low gas exchange efficiency, complex structure and high manufacturing cost of the conventional micro-gravity environment micro-algae closed culture container, the invention provides a micro-gravity environment micro-algae closed culture container, which comprises the following components in part by weight; the culture solution box comprises a wavy solution box top wall and a wavy solution box bottom wall which are made of hydrophobic and breathable materials, a solution box side wall which is connected with the solution box top wall and the solution box bottom wall in a matched mode, and a light source assembly which penetrates through the inside of the culture solution box, wherein the upper half shell and the solution box top wall are matched to form an upper air chamber, the lower half shell and the solution box bottom wall are matched to form a lower air chamber, and an inlet and an outlet are respectively arranged on the culture solution box, the upper air chamber and the lower air chamber. Preferably, the side wall of the liquid tank comprises a first wall plate, a second wall plate, a third wall plate and a fourth wall plate, wherein the upper edge and the lower edge of the first wall plate are in wave shape, the first wall plate and the second wall plate are arranged in parallel, the third wall plate and the fourth wall plate are arranged perpendicular to the first wall plate, two ends of the third wall plate are respectively connected with the left ends of t