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CN-121991784-A - A two module oscillation type resuscitation devices for high-capacity freezing biological agent

CN121991784ACN 121991784 ACN121991784 ACN 121991784ACN-121991784-A

Abstract

The invention belongs to the technical field of biological sample preservation and resuscitation, and provides a two-module oscillation type resuscitation device for a large-capacity frozen biological agent, which comprises a fixed base, wherein the top of the fixed base is movably connected with an oscillation shell box, and one side of the fixed base is fixedly connected with a console; the heating upper module and the heating lower module are both semi-cylindrical and made of Peltier constant temperature metal blocks, the inner walls of the heating upper module and the heating lower module are fixedly provided with temperature sensors, and the inner walls of the bottoms of the fixing bases are fixedly connected with fixing plates. According to the invention, the heating upper module and the heating lower module are both semi-cylindrical Peltier constant-temperature metal blocks, and after the heating upper module and the heating lower module are closed, 360-degree all-round tight package can be formed on a cylindrical sample (such as a 50mL syringe), so that a very large contact area and a high-efficiency heat conduction path far exceeding the traditional water bath or planar heating are provided.

Inventors

  • YANG WEIHUA
  • SONG YONGFENG
  • LIU HUI
  • GUAN WENYU
  • DANG XIAOFEI
  • YIN CHAO
  • Hong Yatian
  • LI YANG

Assignees

  • 济南市中心医院

Dates

Publication Date
20260508
Application Date
20251231

Claims (10)

  1. 1. A two-module oscillating resuscitation device for large volumes of frozen biological agents, characterized by: The device comprises a fixed base (1), wherein the top of the fixed base is movably connected with an oscillation shell box (2), and one side of the fixed base is fixedly connected with a console (3); A heating upper module (4) is arranged in the oscillation shell box (2), and a heating lower module (5) is connected to the bottom of the heating upper module (4) through a hinge; the heating upper module (4) and the heating lower module (5) are semi-cylindrical and made of Peltier constant temperature metal blocks, and temperature sensors are fixedly arranged on the inner walls of the heating upper module and the heating lower module; The inner wall of the bottom of the fixed base (1) is fixedly connected with a fixed plate (6), one side of the fixed plate (6) is fixedly connected with a first servo motor (7), and the movable end of the first servo motor (7) penetrates through the fixed plate (6) and is fixedly connected with a driving turntable (8); one side of the driving turntable (8) is fixedly connected with a centrifugal rod (9), one end of the centrifugal rod (9) is movably connected with a swinging rod (10), one end of the swinging rod (10) is fixedly connected with a half gear (12), and one side of the half gear (12) is connected with a moving rod (14) in a meshed manner; the two ends of the moving rod (14) are fixedly connected with connecting plates (16), and the top ends of the two connecting plates (16) are fixedly connected to the bottom of the oscillation shell box (2); the inside of the oscillation housing box (2) is provided with a shaking device for performing a fan-shaped shaking action on the heating lower module (5).
  2. 2. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: the shaking device comprises a second servo motor (18), a linkage rod (19), a sliding rail (20), a rack (21), a driving gear (22), a rotating rod (23) and a placing block (24); A movable end of the second servo motor (18) penetrates through the oscillating shell box (2) and is fixedly connected with a linkage rod (19), and one end of the linkage rod (19) is movably connected with a sliding track (20); one side of the sliding track (20) is fixedly connected with a rack (21) through a fixed block, the top of the rack (21) is connected with a driving gear (22) in a meshed manner, and the center of the driving gear (22) is fixedly connected with a rotating rod (23); one end of the rotating rod (23) penetrates through the partition plate and is fixedly connected with a placement block (24).
  3. 3. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: and temperature sensors are fixedly arranged on the inner walls of the heating upper module (4) and the heating lower module (5) and used for accurately controlling the heating temperature.
  4. 4. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: The top of the fixed plate (6) is fixedly connected with a sliding block (17), and one side of the fixed plate (6) is fixedly connected with a limiting rod (11); a long groove is formed in the bottom of the oscillation shell box (2), and the top end of the sliding block (17) is connected in the long groove in a sliding way; One side of the fixed plate (6) is fixedly connected with two limiting plates (13) corresponding to the upper part of the limiting rod (11).
  5. 5. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: A sliding groove is formed in one side of the swing rod (10), and one end of the centrifugal rod (9) is movably connected in the sliding groove; One end of the limiting rod (11) is rotatably connected to one side of the swinging rod (10).
  6. 6. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: The movable rod (14) is movably connected between the two limiting plates (13), and two ends of the movable rod (14) respectively penetrate through the two limiting plates (13) to be prolonged; two oscillating springs (15) are fixedly connected between two ends of the two moving rods (14) and the two limiting plates (13).
  7. 7. The two-module oscillating resuscitation device for large volumes of frozen biological agents of claim 1, wherein: The inside of the oscillation shell box (2) is divided into a placing space and a driving space by a partition plate; the shaking device is positioned in the driving space, and the heating upper module (4) and the heating lower module (5) are positioned in the placing space.
  8. 8. A two-module oscillating resuscitation device for large volumes of frozen biological agents according to claim 2, wherein: one end of the linkage rod (19) is movably connected with a linkage sliding block, and one end of the linkage sliding block is slidably connected to the inner wall of the sliding track (20).
  9. 9. A two-module oscillating resuscitation device for large volumes of frozen biological agents according to claim 2, wherein: The rack (21) and the sliding rail (20) are arranged in a cross shape, and a connecting chute is formed in one side of the partition plate; One side of the rack (21) is connected with the inside of the connecting chute in a sliding way.
  10. 10. A two-module oscillating resuscitation device for large volumes of frozen biological agents according to claim 2, wherein: the placement block (24) is semi-cylindrical, and one side of the placement block (24) is connected with a threaded push rod (25) in a threaded manner; one end of the threaded push rod (25) penetrates through the placement block (24) and is connected with a push block (26) in a rotating mode.

Description

A two module oscillation type resuscitation devices for high-capacity freezing biological agent Technical Field The invention belongs to the technical field of biological sample preservation and resuscitation, and particularly relates to a two-module oscillation type resuscitation device for a large-capacity frozen biological agent. Background In the medical field, intestinal bacteria transplantation technology is becoming popular, and brings new hope and approach for the treatment of a plurality of diseases. The enterobacteria liquid is used as a key carrier of the technology, and the preservation and recovery quality of the enterobacteria liquid are directly related to the effectiveness and safety of treatment. Typically, the intestinal fluid is stored in a 50mL syringe or centrifuge tube and frozen in a low temperature environment at-80 ℃ to maximize the activity and stability of the flora. In the use process of the intestinal bacteria liquid, a resuscitation link is important. In order to preserve the intestinal bacterial activity, it is critical to follow an "instant" strategy, i.e. to require complete, uniform thawing of the sample within 1 to 2 minutes. If the resuscitation time exceeds this range, the survival rate of the intestinal bacteria will be significantly reduced, thereby affecting the activity and structure of the flora and ultimately adversely affecting the effectiveness and safety of the treatment. At present, a water bath kettle is generally adopted in a laboratory to carry out the resuscitation operation of the intestinal bacteria liquid, however, the mode has a plurality of obvious defects. First, the thawing rate is extremely slow and the resuscitation efficiency is low. From-80 ℃ to full thawing, typically 15-30 minutes are required. The long thawing process can lead a large amount of intestinal bacteria to die due to the formation of ice crystals in a low-temperature environment for a long time, directly lead to the reduction of the activity of the intestinal bacteria, change the flora structure and seriously influence the subsequent treatment effect. Second, temperature control is not accurate enough. In the running process of the water bath, the water temperature is easy to have larger fluctuation, the intestinal flora is extremely sensitive to the temperature, and the unstable temperature is extremely easy to cause the inactivation of part of the temperature-sensitive flora, so that the integral quality of the intestinal bacteria liquid is further weakened. Furthermore, the problem of uneven heating is prominent. For large volumes of samples (e.g., 50 mL), there is a significant temperature difference between the sample core and the outside, and it is difficult to synchronize the thawing process. The uneven heating condition greatly reduces the activity of the intestinal bacteria in the frozen interior, and the resuscitation effect is greatly reduced. In order to overcome the pollution problem caused by the water bath resuscitating mode, the existing dry type thermostat has been developed. While the dry thermostat avoids to some extent the contamination that may be caused by the water bath, it also suffers from uneven heating when handling large volumes of samples. Because it adopts the static heating mode, can't realize the even heating of sample to do not possess the vibration mixing function, more can't carry out 360 all-round parcel heating to the sample. This results in uneven heating process, low recovery efficiency, and great influence on the activity of flora, and is difficult to meet the high requirements of intestinal fungus transplantation technology on recovery quality. Therefore, the development of a device capable of rapidly, uniformly and effectively resuscitating a large-capacity frozen biological agent has important significance for improving the treatment effect and the safety of the intestinal fungus transplanting technology. The invention aims to provide a two-module oscillation type resuscitation device for large-capacity frozen biological agents, so as to solve the problems of biological sample preservation and resuscitation in the background technology. Disclosure of Invention The invention provides a two-module oscillation type resuscitation device for large-capacity frozen biological agents, which aims to solve the problems of biological sample preservation and resuscitation in the background technology. The technical scheme of the invention is as follows: the invention provides a two-module oscillation type resuscitation device for a large-capacity frozen biological agent, which comprises a fixed base, wherein the top of the fixed base is movably connected with an oscillation shell box, and one side of the fixed base is fixedly connected with a console; A heating upper module is arranged in the oscillation shell box, and the bottom of the heating upper module is connected with a heating lower module through a hinge; The heating upper module and the heati