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CN-121991789-A - Sample tube for nucleic acid detection, sample adding structure of cartridge and sample adding method of sample tube

CN121991789ACN 121991789 ACN121991789 ACN 121991789ACN-121991789-A

Abstract

The invention relates to a sample tube for nucleic acid detection, a sample adding structure of a cartridge and a sample adding method thereof, and belongs to the field of in-vitro diagnosis equipment. The sample tube comprises a tube body and a cover body, wherein a first reserved opening and a second reserved opening which are closed by a pierceable sealing film are respectively formed in the bottom of the tube body and the cover body, so that a double-end sealing structure is formed. The sample adding structure comprises a cartridge body, a sample adding cover, an upper puncturing mechanism and a lower puncturing mechanism. When the sample tube is used, the sample tube is placed in the sample loading cavity of the cartridge body, the sample loading cover is covered and pressed downwards, the sample tube can be driven to move downwards, the lower puncturing mechanism punctures the sealing film of the cover body, and meanwhile, the upper puncturing mechanism punctures the sealing film of the tube bottom, so that the smooth release and sample loading of the sample in a closed environment are realized. The invention thoroughly eliminates the risk of aerosol pollution in the sample adding process through an innovative double-end synchronous puncturing mechanism, ensures the smoothness and completeness of sample adding, has simple and reliable structure, and is easy to realize automatic operation.

Inventors

  • XIA XIAOKAI
  • LI WEI
  • ZHANG SHUXIANG
  • REN YONGLI
  • SHAO HENG

Assignees

  • 上海捷诺生物科技股份有限公司

Dates

Publication Date
20260508
Application Date
20260210

Claims (10)

  1. 1. A sample tube for nucleic acid detection is used for accommodating a detection sample and is characterized in that the sample tube (1) comprises a tube body (11) and a cover body (12), the tube body (11) is provided with a sample storage cavity (111) for storing the sample, the cover body (12) can be assembled on the tube body (11) in a sealing mode, a first reserved opening (112) communicated with the sample storage cavity (111) is formed in the bottom of the tube body (11), a first sealing film (113) capable of being pierced is arranged at the first reserved opening (112) in a sealing mode, a second reserved opening (121) communicated with the sample storage cavity (111) is formed in the cover body (12), and a second sealing film (122) capable of being pierced is arranged at the second reserved opening (121) in a sealing mode.
  2. 2. The sample tube for nucleic acid detection according to claim 1, wherein the diameter of the second preformed hole (121) is larger than the diameter of the first preformed hole (112).
  3. 3. A sample addition structure of a cartridge for nucleic acid detection, comprising the sample tube for nucleic acid detection according to claim 1 or 2, Also included is a method of manufacturing a semiconductor device, The cartridge comprises a cartridge body (2), wherein a sample loading cavity (21) capable of limiting and accommodating a sample tube (1) is arranged in the cartridge body, and an opening (211) is formed in the top of the sample loading cavity (21); A sample loading cover (22) which can be arranged at an opening (211) of the sample loading cavity (21) and is used for opening or closing the opening (211); An upper puncturing mechanism (23) which is arranged on the surface of the sample loading cavity (21) facing the inner side of the sample loading cover (22); a lower puncturing mechanism (24) provided on the inner bottom surface of the sample loading chamber (21); Wherein, sample tube (1) is arranged in sample loading chamber (21) and sample application lid (22) is sealed open (211), pushing down sample application lid (22) can drive sample tube (1) to move down, makes lower puncture mechanism (24) puncture second sealing membrane (122), simultaneously upper puncture mechanism (23) puncture first sealing membrane (113).
  4. 4. The sample loading structure of a cartridge for nucleic acid detection according to claim 3, wherein a closing sleeve (221) is extended from the sample loading chamber (21) toward the sample loading cover (22), an outer peripheral wall of the closing sleeve (221) is in sealing engagement with an inner peripheral wall of the sample loading chamber (21), and the upper puncturing mechanism (23) is provided in a space surrounded by the closing sleeve (221).
  5. 5. The cartridge loading structure for nucleic acid detection according to claim 4, wherein a pushing ring (222) is further provided on a side of the loading chamber (21) facing the loading cap (22), the pushing ring (222) is located inside the closing sleeve (221) and surrounds the periphery of the upper puncturing mechanism (23), and the protruding height of the closing sleeve (221) is larger than the protruding height of the upper puncturing mechanism (23), and the protruding height of the upper puncturing mechanism (23) is larger than the protruding height of the pushing ring (222).
  6. 6. A sample loading structure of a cartridge for nucleic acid detection according to claim 3, wherein the cartridge body (2) is further provided with a sample groove (25), the sample groove (25) is communicated with the bottom of the sample loading chamber (21), and the groove bottom height of the sample groove (25) is lower than the groove bottom height of the sample loading chamber (21).
  7. 7. The sample loading structure of a cartridge for nucleic acid detection according to claim 3, wherein the lower lancing mechanism (24) includes a center lancing member (241) provided and a plurality of circumferential lancing members (242) provided around the center lancing member (241).
  8. 8. The sample loading structure of a cartridge for nucleic acid detection according to claim 3, wherein a raised block (26) is provided on an inner bottom surface of the sample loading chamber (21), the raised block (26) is located on an outer periphery of the lower lancing mechanism (24), and a height of the raised block (26) is lower than a height of the lower lancing mechanism (24).
  9. 9. A loading method based on the loading structure according to any one of claims 3 to 8, comprising the steps of: S1, placing a sample tube (1) with two sealed ends into the sample loading cavity (21) from an opening (211); S2, covering a sample adding cover (22) on an opening (211) of the sample loading cavity (21) so that a sealing space is formed at the upper part of the sample loading cavity (21); S3, continuously pressing down the sample adding cover (22), enabling the tip end of the upper puncturing head to contact and puncture the first sealing film (113) at the bottom of the sample tube (1), forming a vent hole, and simultaneously enabling the second sealing film (122) to be punctured by the puncturing mechanism (241); s4, the pushing ring (222) starts to contact the top of the cover body (12) of the sample tube (1), the pushing ring (222) transmits the downward pressure to the sample tube (1), and in the downward moving process of the sample tube (1), the sealing film is torn and spread by the plurality of peripheral ring puncture pieces (242); S5, the sample flows to the bottom of the sample loading cavity (21) from a second reserved opening (121) with the enlarged bottom under the action of gravity, and flows into a sample groove (25) with a lower position along a gap formed by the heightening block (26) for temporary storage.
  10. 10. The method according to claim 9, characterized in that in step S2, a sealing fit is first formed with the inner wall of the sample loading chamber (21) by means of a closing sleeve (221) on the loading lid (22).

Description

Sample tube for nucleic acid detection, sample adding structure of cartridge and sample adding method of sample tube Technical Field The present invention relates to the field of in vitro diagnostic devices. In particular, the present invention relates to a sample tube for nucleic acid detection, a sample loading structure of a cartridge for nucleic acid detection, and a sample loading method of the sample loading structure of the cartridge. Background Nucleic acid detection is the core means of pathogen detection, and the process generally begins with the loading of a sample. In the prior art, whether it is a manual operation or a semi-automated platform, a common sample application method requires that the cap of the sample tube be opened first, and then the sample be transferred using a pipette or a pipette tip. This uncapping and pipetting process inevitably generates aerosols, which not only present a risk of cross-contamination of the sample, but can also pose a biological safety threat to the operator, especially in the detection of highly pathogenic pathogens. To solve the above problems, various integrated cartridge designs have been proposed in the industry to try to enclose the sample processing steps in one device. Some of these schemes use a single-ended lancing of the sample tube to release the sample. However, when only one end is pierced to cause the sample to flow out, negative pressure is formed in the sample tube, so that the sample is prevented from flowing out smoothly and completely, and inaccuracy of sample adding amount, sample residue or intermittent flowing out are easily caused, so that the accuracy and reliability of subsequent detection are affected. In addition, how to ensure that the puncturing operation itself is performed in a closed environment, and prevent the leakage of droplets or aerosols that may be generated at the moment of puncturing, is still a difficult problem that has not been well solved in the prior art. Therefore, there is an urgent need for a sample loading solution that can achieve full tightness, is pollution-free, and is smooth and reliable in sample loading. Disclosure of Invention The invention aims to provide a sample tube for nucleic acid detection, a sample adding structure of a cartridge and a sample adding method thereof, so as to solve one or all of the technical problems in the prior art. In order to achieve the above object, the present invention provides the following solutions: The invention provides a sample tube for nucleic acid detection, which is used for accommodating a detection sample, and comprises a tube body and a cover body, wherein the tube body is provided with a sample storage cavity for storing the sample, the cover body can be assembled on the tube body in a sealing way, a first reserved opening communicated with the sample storage cavity is formed in the bottom of the tube body, a first sealing film capable of being punctured is arranged at the first reserved opening in a sealing way, a second reserved opening communicated with the sample storage cavity is formed in the cover body, and a second sealing film capable of being punctured is arranged at the second reserved opening in a sealing way. According to one embodiment of the invention, the diameter of the second preformed opening is larger than the diameter of the first preformed opening. The sample adding structure of the cartridge for nucleic acid detection comprises the sample tube for nucleic acid detection, Also included is a method of manufacturing a semiconductor device, The cartridge body is internally provided with a sample loading cavity which can limit and accommodate a sample tube, and the top of the sample loading cavity is provided with an opening; The sample loading cover can be arranged at the opening of the sample loading cavity and is used for opening or closing the opening; The upper puncturing mechanism is arranged on the surface, facing the inner side of the sample loading cavity, of the sample loading cover; the lower puncturing mechanism is arranged on the bottom surface of the inner side of the sample loading cavity; The sample tube is arranged in the sample loading cavity, the sample adding cover seals the opening, the sample tube can be driven to move downwards by pressing the sample adding cover downwards, the lower puncturing mechanism punctures the second sealing film, and meanwhile, the upper puncturing mechanism punctures the first sealing film. According to one embodiment of the invention, a sealing sleeve is extended from one side of the sample loading cavity towards the sample loading cover, the outer peripheral wall of the sealing sleeve is in sealing fit with the inner peripheral wall of the sample loading cavity, and the upper puncturing mechanism is arranged in a space enclosed by the sealing sleeve. According to one embodiment of the invention, a pushing ring is further arranged on one side, facing the sample loading cavity, of the sam