Search

CN-121991795-A - Aerosol culture system and culture method for aerobiotic microalgae

CN121991795ACN 121991795 ACN121991795 ACN 121991795ACN-121991795-A

Abstract

The invention discloses an aerosol culture system and a culture method of aeroponics, which combine the growth characteristics of aeroponics biological films by an atomization culture medium supply mode, so that aeroponics grow on the surface of a biological film carrier in a non-submerged mode, the light receiving condition of the aeroponics is obviously improved, the light utilization efficiency is improved, a stable gas-liquid interface is formed on the surface of the aeroponics biological film, the contact area between gas and the biological film is increased, the absorption of carbon dioxide and the release of oxygen are facilitated, and the gas-liquid mass transfer efficiency in the aeroponics culture process is obviously improved.

Inventors

  • PEI HAIYAN
  • XU SHIQI

Assignees

  • 复旦大学

Dates

Publication Date
20260508
Application Date
20260228

Claims (9)

  1. 1. The aerial fog culture system for the aerobiotic microalgae is characterized by comprising a culture box main body (1), wherein an operation door (9) is hinged to the front end of the culture box main body (1), one side of the culture box main body (1) is connected with an air inlet component, illumination components (8) are arranged on the inner walls of two sides of the culture box main body (1), three groups of bearing components (7) are arranged in the culture box main body (1), a first supporting plate (6) is arranged on the uppermost bearing component and the middle bearing component, grooves are formed in the top surface of the first supporting plate (6), biomembrane carriers (5) are arranged in the grooves, a through hole is formed in one corner of the two grooves, the bottom of the through hole is connected with a drain pipe (10), the bottom of the drain pipe (10) is arranged above a filtering interception component, the filtering component is arranged on the lowermost bearing component, the bottom of the culture box main body (1) is a water collecting area (13), the water collecting area is connected with one end of a liquid supply circulation component, and the liquid supply circulation component is communicated with one end of the culture box main body (1).
  2. 2. The aerosol-generating system for aerobiotic microalgae according to claim 1, wherein the bearing component (7) comprises two rows of symmetrically arranged screws fixed on the inner wall of the incubator main body (1), the screws are connected with a fixed pipe in a threaded manner, and the first supporting plate (6) and the filtering and intercepting component are pressed on the fixed pipe.
  3. 3. The aerosol-generating system of aerobiotic microalgae according to claim 1, wherein the biological film carrier (5) is of a film material or screen structure, the pore diameter or mesh number of the biological film carrier (5) is matched with the cell size and biological film forming characteristics of the cultured aerobiotic microalgae, and the biological film carrier (5) is arranged in the groove.
  4. 4. The aerosol-generating system for aerobiotic microalgae according to claim 3, characterized in that the biofilm carrier (5) is of a multilayer arrangement.
  5. 5. The aerosol-generating system for aerobiotic microalgae according to claim 1, characterized in that the illumination assembly comprises a light lamp fixed on the outer wall of the incubator body (1).
  6. 6. The aerosol culture system of aerobiotic microalgae according to claim 1, wherein the second support plate (12) of the filtering and intercepting component is arranged above the lowest bearing component, a strip-shaped hole is formed in the top surface of the second support plate (12), and an intercepting screen (11) is fixed to the top of the strip-shaped hole.
  7. 7. The aerosol culture system of aerobiotic microalgae according to claim 1, characterized in that the liquid supply circulation assembly comprises a water pump (15) and an atomizer (16), wherein the water inlet end of the water pump (15) is connected with the water collecting area (13), the water outlet end of the water pump (15) is connected with the atomizer (16), the discharge outlet of the atomizer (16) is connected with an atomization conveying pipe (14), the atomization conveying pipe (14) is connected with a discharge nozzle, and the discharge nozzle is arranged above the filtering interception assembly and communicated with the incubator main body (1).
  8. 8. The aerosol-generating system for growing microalgae according to claim 1, wherein the air inlet assembly comprises at least one air inlet pipe (2) connected to the side wall of the incubator body (1), and the other end of the air inlet pipe (2) is connected with an air supply source (4) through a hose (3).
  9. 9. A method of cultivating an aerosol-generating microalgae in accordance with any of claims 1 to 8, comprising the steps of: A. Inoculating: Uniformly coating, spraying or dripping the aerobiotic microalgae liquid on the surface of a biological film carrier (5) to enable the algae to be attached to the biological film carrier (5) to form an initial attachment layer; The biological film carrier (5) can be sterilized or cleaned before inoculation so as to ensure that algae are uniformly attached and reduce the pollution of mixed bacteria; B. Atomizing liquid supply: starting a liquid supply circulation assembly, atomizing the culture medium into fine liquid drops by an atomizer (16), and spraying the fine liquid drops through an atomization conveying pipe (14) and a discharge nozzle to enable the culture medium to periodically or continuously act on the surface of the biological film carrier (5) in an aerosol form; the atomization liquid supply can adopt a continuous mist supply or intermittent mist supply mode, and the atomization amount, the atomization time and the atomization frequency are regulated through the flow regulating component and the timing control component, so that the surface of the biological film is maintained in a non-liquid immersed state; C. recycling: in the culture process, the culture medium which is not absorbed and utilized by the aerobiotic microalgae is guided by a drain pipe (10) layer by layer under the action of gravity, and is collected to a water collecting area (13) after the filter interception component intercepts the fallen algae or impurities; The culture medium collected in the water collecting area (13) is conveyed to the atomizer (16) through the water pump (15) to form a closed circulation liquid supply system, so that the cyclic utilization of the culture medium is realized; D. and (3) gas supply: introducing carbon dioxide gas into the incubator main body (1) through the air inlet component (2) to provide carbon sources required by photosynthesis for the aerobiotic microalgae and maintain the stable gas environment in the incubator; E. Biofilm harvesting: When the aerobiotic microalgae on the biological film carrier (5) grows to a preset biomass or a preset culture period, stopping atomizing liquid supply and gas supply, and collecting the algae attached to the surface of the biological film carrier (5) by a manual or mechanical scraping mode so as to obtain the microalgae biomass.

Description

Aerosol culture system and culture method for aerobiotic microalgae Technical Field The invention relates to the field of microalgae culture and bioengineering, in particular to an aerosol culture system and method for aerobiotic microalgae. Background Microalgae is an important photosynthetic microorganism resource, and has wide application prospect in the fields of biomass energy, biological medicine, functional food, environmental treatment and the like. Aerothrice algae are a group of microalgae that can grow on solid surfaces or under air-water interface conditions and adhere in the form of biofilms. It should be noted that, the aerobiotic algae can not only grow under the gas phase condition, but also can be cultivated under a certain condition by adopting the traditional liquid cultivation mode. However, in liquid submerged culture conditions, the growth efficiency of aerobiotic algae is generally low, and its physiological advantages are difficult to fully develop. In particular, in a liquid culture system, the aerobiotic algae is converted into a suspension or semi-suspension state from a biomembrane form which is originally suitable for adhesion growth, the interface of the aerobiotic algae, which is in direct contact with air, is obviously reduced, the carbon dioxide acquisition and the oxygen release mainly depend on a liquid phase mass transfer process, and the gas exchange efficiency is obviously reduced. Meanwhile, the light distribution in the liquid system is uneven, the mutual shielding phenomenon among algae under the high-density culture condition is aggravated, and the photosynthesis efficiency and biomass accumulation of the aerobiotic algae are further limited. In addition, continuous aeration or mechanical stirring is usually required in the liquid culture process to maintain the suspended state of the algae, which not only increases energy consumption, but also easily causes mechanical disturbance to the aerobiotic algae which is originally suitable for adhesion growth, and is unfavorable for forming a stable and uniform biological film structure. In addition, continuous aeration or mechanical agitation is often required during liquid culture to maintain the algae in suspension. Further, in the liquid culture system, the harvest process of the aerobiotic algae also has obvious defects. As algae are dispersed and suspended in a large amount of culture medium, solid-liquid separation is usually needed by means of centrifugation, filtration and the like after the culture is finished, the operation steps are complex, the energy consumption is high, and the algae are easy to lose or influence the activity in the treatment process. The harvest mode using liquid phase separation as a core is not matched with the characteristic that the aerobiotic algae is suitable for adhesion growth and concentrated distribution of biological membranes, and the overall culture efficiency and the economy are further reduced. Therefore, aiming at the characteristics that the gas-borne algae has liquid culture feasibility, is more suitable for high-efficiency growth under the condition of gas phase or gas-liquid interface and has higher requirements on low energy consumption and simplified operation in a harvesting link, the development of a gas-borne algae culture method which can stably provide nutrition under the non-liquid immersion condition, has high illumination utilization and high gas-liquid mass transfer efficiency and is beneficial to the high-efficiency harvesting of the algae is needed to be developed, so that the substantial improvement of the gas-borne algae culture efficiency and the overall application value is realized. Disclosure of Invention The invention aims to provide an aerosol culture system and an aerosol culture method for aerobiotic microalgae, which solve the problems. In order to solve the technical problems, the invention adopts the following technical scheme: The invention discloses an aerosol culture system for aerobiotic microalgae, which comprises a culture box main body, wherein the front end of the culture box main body is hinged with an operation door, one side of the culture box main body is connected with an air inlet assembly, the inner walls of the two sides of the culture box main body are respectively provided with an illumination assembly, the interior of the culture box main body is provided with an upper group of bearing assemblies, a middle group of bearing assemblies and a lower group of bearing assemblies, the uppermost bearing assemblies and the middle bearing assemblies are respectively provided with a first supporting plate, the top surface of the first supporting plate is provided with a groove, biomembrane carriers are arranged in the grooves, one corner of the two grooves is provided with a through hole, the bottom of the through hole is connected with a drain pipe, the bottom of the drain pipe at the lowest part is arranged above a filtering intercepti