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CN-121991797-A - Automatic sampling device for embryo implantation screening

CN121991797ACN 121991797 ACN121991797 ACN 121991797ACN-121991797-A

Abstract

The invention relates to the technical field of sampling devices, in particular to an automatic sampling device for embryo implantation screening, which comprises a processing table, wherein a processing mechanism is arranged on the processing table, the processing mechanism comprises a shading cover which moves on the processing table, the shading cover is integrated with a sampling mechanism, an exhaust pipe is arranged in the shading cover, a culture dish is arranged on the processing table, an exhaust mechanism is moved on one side of the shading cover, the exhaust mechanism comprises an air storage box arranged on one side of the processing table, and the invention aims to provide a device for synchronously driving an exhaust plate to move in the air storage box through a connecting plate in the process of pressing down the shading cover.

Inventors

  • JIN PANPAN
  • XIE JUN
  • LV HUIMIN
  • XiaHou Yaling
  • GAO DONGQIN
  • LI XUEWEI

Assignees

  • 山西医科大学

Dates

Publication Date
20260508
Application Date
20260210

Claims (8)

  1. 1. An automatic sampling device for embryo implantation screening, comprising a treatment table (1), characterized in that the treatment table (1) is provided with a treatment mechanism (2); The processing mechanism (2) comprises a shading cover (21) which moves on the processing table (1), a sampling mechanism (23) is integrated on the shading cover (21), an exhaust pipe (24) is arranged in the shading cover (21), a culture dish (25) is arranged on the processing table (1), and an exhaust mechanism (3) is moved on one side of the shading cover (21); The exhaust mechanism (3) comprises an air storage box (31) arranged on one side of the treatment table (1), an exhaust plate (36) is arranged in the air storage box (31) in a sliding mode, a first exhaust pipe (35) is arranged at one end of the air storage box (31), one end of the first exhaust pipe (35) is communicated with the exhaust pipe (24), a second exhaust pipe (34) is arranged at the bottom of the first exhaust pipe (35), and a cleaning mechanism (4) is arranged at one end of the second exhaust pipe (34); The cleaning mechanism (4) comprises a working cavity (41) arranged inside the treatment table (1), a piston (42) is movably arranged inside the working cavity (41), a liquid storage cylinder (43) is sleeved outside the piston (42), a third exhaust pipe (46) is communicated with one end of the working cavity (41), a liquid discharge pipe (47) is communicated with one side of the liquid storage cylinder (43), one end of the third exhaust pipe (46) is communicated with the liquid discharge pipe (47), and one end of the liquid discharge pipe (47) is communicated with the sampling mechanism (23).
  2. 2. An automatic sampling device for embryo implantation screening according to claim 1, characterized in that the treatment table (1) is provided with an electric telescopic rod (22), the output end of the electric telescopic rod (22) is connected with a shading cover (21), a sealing groove is arranged in the treatment table (1), and the size of the sealing groove is adapted to the shading cover (21).
  3. 3. An automatic sampling device for embryo implantation screening according to claim 1, characterized in that the sampling means (23) comprises a sampling tube (231) mounted on a light shielding cover (21), one end of the liquid discharge tube (47) is communicated with the sampling tube (231), a pressure valve is mounted at the place where the liquid discharge tube (47) is communicated with the sampling tube (231), an adsorption tube (232) is mounted at one side of the sampling tube (231), and a laser system and a microscopic imaging auxiliary component are integrated on the sampling means (23).
  4. 4. An automatic sampling device for embryo implantation screening according to claim 1, characterized in that a connecting plate (32) is fixed on one side of the light shielding cover (21), one end of the connecting plate (32) penetrates through the gas storage box (31) to be connected with the gas exhaust plate (36), one end of the gas storage box (31) is provided with a gas inlet pipe (33), the gas inlet pipe (33) is communicated with external gas storage equipment, and a first one-way valve is installed inside the gas inlet pipe (33).
  5. 5. An automatic sampling device for embryo implantation screening according to claim 1, wherein the first exhaust tube (35) is internally provided with a second one-way valve, one end of the first exhaust tube (35) is communicated with a hose (37), and one end of the hose (37) penetrates through the light shielding cover (21) and is communicated with the exhaust tube (24).
  6. 6. An automatic sampling device for embryo implantation screening according to claim 5, wherein the second exhaust tube (34) is provided inside the gas storage tank (31) and is positioned lower than the first exhaust tube (35), and one end of the second exhaust tube (34) penetrates the treatment table (1) and is communicated with the working chamber (41).
  7. 7. An automatic sampling device for embryo implantation screening according to claim 1, characterized in that the reservoir (43) is internally provided with a spring (45), one end of the spring (45) is connected with the piston (42), the other end of the spring (45) is connected with the inner wall of the reservoir (43), one end of the reservoir (43) is communicated with a feeding tube (44), and the feeding tube (44) is internally provided with a third one-way valve.
  8. 8. An automatic sampling device for embryo implantation screening according to claim 1, wherein the third exhaust tube (46) is internally provided with a pressure valve, and the drain tube (47) is provided with a fourth one-way valve at the position where it communicates with the reservoir (43).

Description

Automatic sampling device for embryo implantation screening Technical Field The invention relates to the technical field of sampling devices, in particular to an automatic sampling device for embryo implantation screening. Background Genetic screening before embryo implantation is a key link in auxiliary reproduction technology, and embryo trophoblast cells are subjected to biopsy and genetic analysis to screen out embryos with normal chromosomes or without specific genetic diseases for transplantation, so that the clinical pregnancy rate can be remarkably improved, the flow rate and the birth defect risk can be reduced, currently, embryo biopsy mainly depends on embryologists to perform manual operation under an inverted microscope, and the standard flow generally comprises the steps of transferring the embryos from an incubator to a micromanipulator, using acidic liquid or laser to open on a transparent belt, and then using a microinjection needle or a biopsy needle to suck a plurality of trophoblast cells; When the conventional sampling device is used, after an embryo leaves an incubator of a low-oxygen mixed gas, the embryo is exposed to laboratory air, the oxygen partial pressure in the air is far higher than that of a physiological environment, and the concentration of carbon dioxide is insufficient, so that the pH of a culture solution is rapidly increased, and meanwhile, the temperature is reduced and the liquid evaporates, and the environmental fluctuation can cause oxidative stress and metabolic interference on the embryo, so that the development potential and the survival rate of the embryo after biopsy are seriously influenced. Disclosure of Invention The invention aims to provide an automatic sampling device for embryo implantation screening, which utilizes a connecting plate to synchronously drive an exhaust plate to move in a gas storage box in the process of pressing down a shading cover, and when the exhaust plate moves above a first exhaust pipe, gas in the gas storage box is conveyed into the exhaust pipe through the first exhaust pipe and a hose, so that low-oxygen mixed gas is synchronously sprayed out in the descending process, the gas environment of a sampling area is replaced before sealing, and the influence of the environment in sampling is reduced. In order to achieve the aim, the invention provides the following technical scheme that the automatic sampling device for embryo implantation screening comprises a processing table, wherein a processing mechanism is arranged on the processing table; The processing mechanism comprises a shading cover which moves on a processing table, a sampling mechanism is integrated on the shading cover, an exhaust pipe is arranged in the shading cover, a culture dish is arranged on the processing table, and an exhaust mechanism is moved on one side of the shading cover; The exhaust mechanism comprises an air storage box arranged on one side of the treatment table, an exhaust plate is arranged in the air storage box in a sliding mode, a first exhaust pipe is arranged at one end of the air storage box, one end of the first exhaust pipe is communicated with the exhaust pipe, a second exhaust pipe is arranged at the bottom of the first exhaust pipe, and one end of the second exhaust pipe is communicated with the cleaning mechanism; The cleaning mechanism comprises a working cavity arranged inside the processing table, a piston is movably arranged inside the working cavity, a liquid storage cylinder is sleeved outside the piston, one end of the working cavity is communicated with a third exhaust pipe, one side of the liquid storage cylinder is communicated with a liquid discharge pipe, one end of the third exhaust pipe is communicated with the liquid discharge pipe, and one end of the liquid discharge pipe is communicated with the sampling mechanism. Preferably, the processing bench is provided with an electric telescopic rod, the output end of the electric telescopic rod is connected with the shading cover, a sealing groove is formed in the processing bench, and the size of the sealing groove is matched with the shading cover. Preferably, the sampling mechanism comprises a sampling tube arranged on the shading cover, one end of the liquid discharge tube is communicated with the sampling tube, a pressure valve is arranged at the communication position of the liquid discharge tube and the sampling tube, an adsorption tube is arranged on one side of the sampling tube, and the sampling mechanism is integrated with a laser system and a microscopic imaging auxiliary assembly. Preferably, a connecting plate is fixed on one side of the shading cover, one end of the connecting plate penetrates through the gas storage box and is connected with the exhaust plate, an air inlet pipe is arranged at one end of the gas storage box and is communicated with external gas storage equipment, and a first one-way valve is arranged in the air inlet pipe. Preferably, the second