CN-121991799-A - Aerobic microorganism activity detection device and evaluation method based on micro-nano bubbles

CN121991799ACN 121991799 ACN121991799 ACN 121991799ACN-121991799-A

Abstract

The invention discloses an aerobic microorganism activity detection device and an evaluation method based on micro-nano bubbles, wherein the device comprises a gas cylinder (1), a micro-nano bubble generator (2), a water storage tank (3), an electric atomizer (4), a curing bin (5), a reciprocating air pump (6), a gas chromatograph (7), a culture dish (8), a nutrient solution injector (9), a temperature sensor (10), an air pressure sensor (11), a constant-temperature water bath pot (13) and a waterway valve (14). The micro-nano bubble generator (11) generates micro-nano bubble water rich in oxygen, the micro-nano bubble water is sprayed into the maintenance bin (5) through the electric atomizer (4) to culture aerobic microorganisms, the temperature is regulated and controlled by the constant-temperature water bath, the nutrient solution is injected into the culture solution through the nutrient solution injector (9) to promote the metabolic activity of the microorganisms, after maintenance, the gas in the maintenance bin (5) is collected through the reciprocating air pump (6), and the oxygen consumption and the carbon dioxide generation amount are detected by the gas chromatography instrument (7), so that the microbial activity is evaluated.

Inventors

LI GANG
JIANG BOYANG
XU XIUPING
HAN JIAWANG
YANG YING

Assignees

中钢集团马鞍山矿山研究总院股份有限公司
山东科技大学

Dates

Publication Date: 20260508
Application Date: 20260205

Claims (10)

1. An aerobic microorganism activity detection device based on micro-nano bubbles is characterized by comprising a gas cylinder (1), a micro-nano bubble generator (2), a water storage tank (3), an electric atomizer (4), a maintenance bin (5), a reciprocating type sucking pump (6), a gas chromatograph instrument (7), a culture dish (8), a nutrient solution injector (9), a temperature sensor (10), an air pressure sensor (11), a constant-temperature water bath pot (13) and a waterway valve (14), wherein the gas cylinder (1) is connected to the air inlet end of the micro-nano bubble generator (2) through a gas pipe, the water inlet end of the micro-nano bubble generator (2) is connected with a water source, the water outlet end of the micro-nano bubble generator (2) is connected with the water storage tank (3), the outlet of the water storage tank (3) is connected with the electric atomizer (4) through a water conveying pipeline, the waterway valve (14) is arranged on the water conveying pipeline and used for controlling the on-off of the water conveying pipeline, the electric atomizer (4) is provided with an electric atomizer container (12), the bin (5) is a closed container made of transparent glass or corrosion-resistant plastic, so that the culture dish (5) is placed in the water storage bin (5) to be placed outside the maintenance bin (9) in a maintenance process of the maintenance bin (5) so as to be convenient for observing the inside the maintenance bin (5), the temperature sensor (10) and the air pressure sensor (11) are arranged in the curing bin (5), the outlet of the curing bin (5) is connected with the reciprocating air pump (6) through an air pipe, the outlet of the reciprocating air pump (6) is connected with the gas chromatograph (7), the outlet of the electric atomizer (4) is connected with the inlet of the curing bin (5) through a pipeline, the connecting pipeline of the reciprocating air pump (6) and the curing bin (5) is in a sealing design, the air extraction amount is adjustable, the tightness of the curing bin (5) is monitored in real time through the air pressure sensor (11), and the air pressure in the bin is maintained within +/-5% of the external atmospheric pressure difference.
2. The aerobic microbial activity detection device based on micro-nano bubbles according to claim 1, wherein the reciprocating air pump (6) adopts a one-way pump.
3. The aerobic microbial activity detection device based on micro-nano bubbles, as set forth in claim 1, is characterized in that the volume of the water storage tank (3) is 5L-10L for temporarily storing micro-nano bubble water, the volume of the electric atomizer water storage container (12) is fixed to be 100 mL-200 mL, and the volume of the maintenance bin (5) is 1L-5L.
4. A micro-nano bubble-based aerobic microorganism activity detection and evaluation method is characterized by adopting a self-designed micro-nano bubble-based aerobic microorganism activity detection device, wherein the micro-nano bubble-based aerobic microorganism activity detection device comprises a gas cylinder (1), a micro-nano bubble generator (2), a water storage tank (3), an electric atomizer (4), a maintenance bin (5), a reciprocating suction pump (6), a gas chromatograph (7), a culture dish (8), a nutrient solution injector (9), a temperature sensor (10), a pressure sensor (11), a constant-temperature water bath pot (13) and a waterway valve (14), the gas cylinder (1) is connected to the gas inlet end of the micro-nano bubble generator (2) through a gas pipe, the water inlet end of the micro-nano bubble generator (2) is connected with a water source, the water outlet end of the micro-nano bubble generator (2) is connected with the water storage tank (3), the outlet of the micro-nano bubble generator (3) is connected with the electric atomizer (4) through a water pipe, the waterway valve (14) is arranged on the water pipe for controlling the on-off of the transmission path, the electric atomizer (4) is provided with the electric atomizer (12) for observing the water storage bin (5) in a transparent glass container (5) or a transparent plastic container (5) used for culturing and inoculating the culture bin, the maintenance bin (5) is externally connected with a nutrient solution injector (9), the maintenance bin (5) is arranged in a constant-temperature water bath (13), a temperature sensor (10) and an air pressure sensor (11) are arranged in the maintenance bin (5), an outlet of the maintenance bin (5) is connected with a reciprocating air pump (6) through an air pipe, an outlet of the reciprocating air pump (6) is connected with a gas chromatographic instrument (7), and the reciprocating air pump (6) adopts a one-way pump; the method for detecting and evaluating the activity of the aerobic microorganisms comprises the following steps: S1, preparing and storing micro-nano bubble water, namely introducing oxygen in a gas cylinder (1) into a micro-nano bubble generator (2), controlling air inflow and water inflow to generate micro-nano bubble water rich in oxygen, and flowing into a water storage tank (3) for temporary storage; s2, atomizing micro-nano bubble water, namely conveying the micro-nano bubble water in the water storage tank (3) to the electric atomizer (4) through a water conveying pipeline for atomization, and spraying the atomized water into the maintenance bin (5), wherein the water conveying pipeline is regulated to be on-off through a waterway valve (14) to ensure stable atomization process, and the atomization particle size is controlled within a range of 50-200 mu m; S3, culturing aerobic microorganisms, namely inoculating oxygen microorganisms for mine dust inhibition on a culture dish (8) in a maintenance bin (5), and controlling to inject the same amount of culture solution into the culture dish by scales to provide nutrition for the microorganisms by utilizing a nutrient solution injector (9) connected with the outside of the maintenance bin (5), wherein the culture solution comprises a carbon source, a nitrogen source, inorganic salt and growth factors so as to maintain the normal metabolism of the microorganisms; S4, monitoring and regulating the maintenance environment in real time, namely placing the maintenance bin (5) in a constant-temperature water bath (13), regulating the temperature in the maintenance bin (5) through the constant-temperature water bath (13) so as to simulate the mine environment and research the influence of the temperature on the activity of microorganisms; S5, collecting metabolic gas, namely after curing for a set time, extracting the gas from the curing bin (5) by using a reciprocating air pump (6); s6, detecting gas components, namely introducing the extracted gas into a gas chromatograph (7), analyzing the gas components and the content, and mainly detecting the concentration change of oxygen and carbon dioxide, wherein the gas chromatograph (7) provides high-precision data for quantifying the metabolic activity of microorganisms; S7, microbial activity evaluation: ① Based on the gas composition variation, the oxygen consumption amount and the carbon dioxide production amount are calculated, and further the microbial respiratory RQ value rq= (carbon dioxide production amount/oxygen consumption amount) ×100%; ② Based on the metabolic characteristics of aerobic microorganisms, quantitative evaluation of microbial activity is performed by calculating the O 2 consumption rate and the CO 2 generation rate, and the formula is as follows: microbial Activity index= (Δco 2 /Δt）/（ΔO 2 /Δt) Wherein DeltaCO 2 and DeltaO 2 are respectively the volume concentration changes of CO 2 and O 2 in unit time,%,. DELTA.t is the culture time, and h.
5. The method for detecting and evaluating the aerobic microbial activity based on the micro-nano bubbles according to claim 4 is characterized in that the volume of the water storage tank (3) is 5-10L and is used for temporarily storing micro-nano bubble water, the volume of the electric atomizer water storage container (12) is fixed to be 100-200 mL, and the volume of the maintenance bin (5) is 1-5L.
6. The method for detecting and evaluating the activity of the aerobic microorganisms based on the micro-nano bubbles according to claim 4, wherein in the step S4, the constant temperature water bath (13) is used for adjusting the temperature in the maintenance bin (5), the temperature range is controlled to be 15-75 ℃, and the temperature control precision is +/-0.1 ℃.
7. The method of claim 4, wherein in step S3, the composition of the culture solution comprises glucose as a carbon source, ammonium salt as a nitrogen source, phosphate as an inorganic salt, and vitamins as growth factors.
8. The method for detecting and evaluating the aerobic microbial activity based on the micro-nano bubbles according to claim 4, wherein in the step S1, the concentration of oxygen in the gas cylinder (1) is not lower than 99%, in the step S5, the pumping speed of the reciprocating pump (6) is controlled to be 10 mL/min-100 mL/min, and the pumping time is fixed to be 1 minute, so that the representativeness and the repeatability of a gas sample are ensured.
9. The method for detecting and evaluating the activity of the aerobic microorganisms based on the micro-nano bubbles according to claim 4, wherein the tightness of the maintenance bin (5) is monitored in real time by an air pressure sensor (11), and the air pressure in the bin is maintained within +/-5% of the external atmospheric pressure difference so as to avoid gas leakage and ensure the accuracy of gas detection.
10. The method for detecting and evaluating the activity of aerobic microorganisms based on micro-nano bubbles according to claim 4, wherein the nutrient solution injected by the nutrient solution injector (9) is glucose with the concentration of 10-20 g/L, the nitrogen source is ammonium sulfate with the concentration of 5-10 g/L, the inorganic salt comprises monopotassium phosphate with the concentration of 1-2 g/L, the growth factor is vitamin B group and the concentration of 0.1-0.5 g/L so as to ensure the consistency of the growth of microorganisms.

Description

Aerobic microorganism activity detection device and evaluation method based on micro-nano bubbles Technical Field The invention relates to the technical field of microbial activity detection and evaluation, in particular to an aerobic microbial activity detection device and an evaluation method based on micro-nano bubbles, which are particularly suitable for activity quantitative evaluation in microbial dust suppression and coal spontaneous combustion prevention and control engineering in a mine environment. Background The application of aerobic microorganisms in the field of mining safety has become an important direction of green mine construction, and the core functions of dust solidification (such as secretion of extracellular polymers to form agglomerates), oxygen consumption (reduction of oxygen concentration in spontaneous combustion environment of coal), active component degradation (destruction of spontaneous combustion chain reaction of coal) and the like are realized through metabolic activities, so that the engineering implementation effect is directly determined. However, the dynamic change of the microbial activity is obviously affected by environmental factors (such as mine humiture, mine dust components and harmful gases), and the on-site flora regulation is guided by accurate detection. At present, the traditional microorganism activity detection method mainly depends on liquid phase culture or agar plate counting in a laboratory environment, and has the problems of low oxygen transmission efficiency, insufficient gas interference control, long detection period and the like. The conventional aeration method has the defects that the transfer rate of bubble dissolved oxygen is low, so that the evaluation result of microorganism metabolic activity is inaccurate, external air is easy to permeate in an open or semi-closed culture system, the accurate monitoring of the concentration of oxygen and carbon dioxide in a culture bin is affected, the sensitivity to the change of trace gas is low, the plate counting method needs a culture period of 3-7 days, the liquid-phase OD value method is severely interfered by mineral dust particles, and the engineering requirement of rapid feedback and real-time adjustment of a mine site cannot be met. The potential of the micro-nano bubble technology in the field of aerobic microbial activity detection is not yet excavated, the prior art lacks a system scheme combining micro-nano bubble oxygen supply, atomization spraying, closed culture and gas chromatography detection, a gas-liquid-solid phase multi-parameter linkage detection system is not established, the gas concentration change caused by microbial metabolism and physical adsorption is difficult to distinguish, and quick and high-precision quantitative evaluation of microbial activity is difficult to realize. Therefore, it is needed to develop an activity evaluation device and method capable of enhancing oxygen transfer efficiency, precisely controlling culture conditions and realizing nondestructive detection of gas components, so as to support the large-scale application of the microbial technology in mining safety. Disclosure of Invention The invention aims at solving the problems existing in the prior art, and provides an aerobic microbial activity detection device based on micro-nano bubbles, so as to solve the problems that in the traditional microbial activity detection technology, the microbial activity detection efficiency is low, the microbial metabolic activity is difficult to accurately quantify, the external gas component interferes with the test result, the accuracy is poor, and the gas component change cannot be monitored in real time. The invention further aims to provide an aerobic microorganism activity detection and evaluation method based on micro-nano bubbles. In order to achieve the above purpose, the invention relates to an aerobic microorganism activity detection device and an evaluation method based on micro-nano bubbles, which are implemented by adopting the following technical scheme. The invention relates to an aerobic microorganism activity detection device based on micro-nano bubbles, which is characterized by comprising a gas cylinder, a micro-nano bubble generator, a water storage tank, an electric atomizer, a maintenance bin, a reciprocating air pump, a gas chromatographic instrument, a culture dish, a nutrient solution injector, a temperature sensor, an air pressure sensor, a constant-temperature water bath kettle and a waterway valve; the air bottle is connected to the air inlet end of the micro-nano bubble generator through an air pipe, the water inlet end of the micro-nano bubble generator is connected with a water source, the water outlet end of the micro-nano bubble generator is connected with a water storage tank, the outlet of the water storage tank is connected with an electric atomizer through a water pipeline, a waterway valve is arranged on the water pipeline and